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    Methods and systems for detection and isolation of a nucleotide sequence
    3.
    发明申请
    Methods and systems for detection and isolation of a nucleotide sequence 审中-公开
    检测和分离核苷酸序列的方法和系统

    公开(公告)号：US20050053942A1

    公开(公告)日：2005-03-10

    申请号：US10601140

    申请日：2003-06-20

    申请人： Sakari Kauppinen ,  Nana Jacobsen

    发明人： Sakari Kauppinen ,  Nana Jacobsen

    IPC分类号： C12N15/10 ,  C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12Q1/6834 ,  C12Q2525/173 ,  C12Q2565/519 ,  C12Q2525/101

    摘要： A method for isolating nucleic acid molecules having a repeating nucleotide sequence or a homopolymeric nucleotide sequence, e.g. a poly A stretch, is described. In particular, the method uses oligomeric capture probes spiked with various amounts of locked nucleic acid (LNA). The invention further describes methods for the isolation of RNA molecules, for example polyadenylated mRNA molecules, which overcome the problems of rapid RNA degradation during isolation and analysis of such nucleic acid molecules. This is of major clinical and diagnostic importance, especially when dealing with RNA viruses, such as retroviruses or when analyzing rare or low-abundant mRNAs or mRNAs from biopsies or tissues enriched with RNases.

    摘要翻译： 用于分离具有重复核苷酸序列或均聚核苷酸序列的核酸分子的方法，例如 描述了聚A拉伸。 特别地，该方法使用掺有各种量的锁定核酸（LNA）的寡聚捕获探针。 本发明还描述了分离RNA分子的方法，例如多聚腺苷酸化的mRNA分子，其克服了在分离和分析这些核酸分子期间快速RNA降解的问题。 这在临床和诊断方面具有重要的意义，特别是在处理RNA病毒（如逆转录病毒）或分析来自活检组织或富含核糖核酸酶的组织的稀有或低丰度的mRNA或mRNA时。

    Methods and systems for detection and isolation of a nucleotide sequence
    6.
    发明申请
    Methods and systems for detection and isolation of a nucleotide sequence 审中-公开
    检测和分离核苷酸序列的方法和系统

    公开(公告)号：US20080096191A1

    公开(公告)日：2008-04-24

    申请号：US11893108

    申请日：2007-08-14

    申请人： Sakari Kauppinen ,  Nana Jacobsen

    发明人： Sakari Kauppinen ,  Nana Jacobsen

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12Q1/6834 ,  C12Q2525/173 ,  C12Q2565/519 ,  C12Q2525/101

    摘要： A method for isolating nucleic acid molecules having a repeating nucleotide sequence or a homopolymeric nucleotide sequence, e.g. a poly A stretch, is described. In particular, the method uses oligomeric capture probes spiked with various amounts of locked nucleic acid (LNA). The invention further describes methods for the isolation of RNA molecules, for example polyadenylated mRNA molecules, which overcome the problems of rapid RNA degradation during isolation and analysis of such nucleic acid molecules. This is of major clinical and diagnostic importance, especially when dealing with RNA viruses, such as retroviruses or when analyzing rare or low-abundant mRNAs or mRNAs from biopsies or tissues enriched with RNases.

    摘要翻译： 用于分离具有重复核苷酸序列或均聚核苷酸序列的核酸分子的方法，例如 描述了聚A拉伸。 特别地，该方法使用掺有各种量的锁定核酸（LNA）的寡聚捕获探针。 本发明还描述了分离RNA分子的方法，例如多聚腺苷酸化的mRNA分子，其克服了在分离和分析这些核酸分子期间快速RNA降解的问题。 这在临床和诊断方面具有重要的意义，特别是在处理RNA病毒（如逆转录病毒）或分析来自活检组织或富含核糖核酸酶的组织的稀有或低丰度的mRNA或mRNA时。