THERMOSTABLE CATALASE
    2.
    发明申请
    THERMOSTABLE CATALASE 有权
    热稳定催化剂

    公开(公告)号:US20100330646A1

    公开(公告)日:2010-12-30

    申请号:US12918017

    申请日:2009-02-18

    CPC分类号: C12N9/0065 C12Y111/01006

    摘要: An object is to efficiently produce thermostable catalase at low cost by expressing it as a recombinant protein in large quantity.A recombinant microorganism capable of efficiently expressing thermostable catalase can be provided by obtaining a DNA necessary for efficiently producing it as a recombinant protein, and the thermostable catalase can be efficiently produced at low cost by cultivating the obtained recombinant microorganism. Hydrogen peroxide can be efficiently decomposed at low cost, even at high temperature, by treating a solution containing hydrogen peroxide with the thermostable catalase of the present invention.

    摘要翻译: 目的是通过将其大量表达为重组蛋白,以低成本有效地产生热稳定的过氧化氢酶。 可以通过获得作为重组蛋白质有效生产所必需的DNA来提供能够有效表达耐热性过氧化氢酶的重组微生物,通过培养所获得的重组微生物,可以低成本有效地制备热稳定性过氧化氢酶。 通过用本发明的耐热性过氧化氢酶处理含有过氧化氢的溶液,即使在高温下也能以低成本有效地分解过氧化氢。

    Thermostable catalase
    4.
    发明授权
    Thermostable catalase 有权
    耐热过氧化氢酶

    公开(公告)号:US08975053B2

    公开(公告)日:2015-03-10

    申请号:US12918017

    申请日:2009-02-18

    IPC分类号: C12N9/08 C07H21/04

    CPC分类号: C12N9/0065 C12Y111/01006

    摘要: An object is to efficiently produce thermostable catalase at low cost by expressing it as a recombinant protein in large quantity.A recombinant microorganism capable of efficiently expressing thermostable catalase can be provided by obtaining a DNA necessary for efficiently producing it as a recombinant protein, and the thermostable catalase can be efficiently produced at low cost by cultivating the obtained recombinant microorganism. Hydrogen peroxide can be efficiently decomposed at low cost, even at high temperature, by treating a solution containing hydrogen peroxide with the thermostable catalase of the present invention.

    摘要翻译: 目的是通过将其大量表达为重组蛋白,以低成本有效地产生热稳定的过氧化氢酶。 可以通过获得作为重组蛋白质有效生产所必需的DNA来提供能够有效表达耐热性过氧化氢酶的重组微生物,通过培养所获得的重组微生物,可以低成本有效地制备热稳定性过氧化氢酶。 通过用本发明的耐热性过氧化氢酶处理含有过氧化氢的溶液,即使在高温下也能以低成本有效地分解过氧化氢。

    KETOREDUCTASE MUTANT
    5.
    发明申请
    KETOREDUCTASE MUTANT 有权
    KETOREDUCTASE突变体

    公开(公告)号:US20130116416A1

    公开(公告)日:2013-05-09

    申请号:US13699206

    申请日:2010-05-21

    IPC分类号: C12N9/02 C12N1/20

    摘要: Disclosed are a ketoreductase mutant which can be used for an efficient production of daunorubicin derivatives, a DNA encoding the mutant, a transformant prepared by introducing the DNA thereinto to produce a daunorubicin derivative, and a process of producing a daunorubicin derivative using the transformant. The ketoreductase mutant has an amino acid sequence in which one amino acid residue or two or more amino acid residues selected from the group consisting of amino acids located at positions corresponding to the 42nd, 149th, 153rd, 270th, and 306th amino acids in the amino acid sequence of a ketoreductase (EvaE) from a chlororemomycin-producing bacterium (Amycolatopsis orientalis) are substituted with another amino acid residues.

    摘要翻译: 公开了可用于有效生产柔红霉素衍生物的酮还原酶突变体,编码突变体的DNA,通过将DNA引入其中以产生柔红霉素衍生物而制备的转化体,以及使用转化体产生柔红霉素衍生物的方法。 酮还原酶突变体具有氨基酸序列,其中选自氨基酸中的一个氨基酸残基或两个或更多个氨基酸残基,所述氨基酸位于与氨基酸中第42,149,153,270和306位氨基酸相对应的位置的氨基酸 来自产生氯霉素的细菌(Amycolatopsis orientalis)的酮还原酶(EvaE)的酸序列被另一个氨基酸残基取代。

    Ketoreductase mutant
    8.
    发明授权
    Ketoreductase mutant 有权
    酮还原酶突变体

    公开(公告)号:US08697409B2

    公开(公告)日:2014-04-15

    申请号:US13699206

    申请日:2010-05-21

    IPC分类号: C12P19/56

    摘要: Disclosed are a ketoreductase mutant which can be used for an efficient production of daunorubicin derivatives, a DNA encoding the mutant, a transformant prepared by introducing the DNA thereinto to produce a daunorubicin derivative, and a process of producing a daunorubicin derivative using the transformant. The ketoreductase mutant has an amino acid sequence in which one amino acid residue or two or more amino acid residues selected from the group consisting of amino acids located at positions corresponding to the 42nd, 149th, 153rd, 270th, and 306th amino acids in the amino acid sequence of a ketoreductase (EvaE) from a chlororemomycin-producing bacterium (Amycolatopsis orientalis) are substituted with another amino acid residues.

    摘要翻译: 公开了可用于有效生产柔红霉素衍生物的酮还原酶突变体,编码突变体的DNA,通过将DNA引入其中以产生柔红霉素衍生物而制备的转化体,以及使用转化体产生柔红霉素衍生物的方法。 酮还原酶突变体具有氨基酸序列,其中选自氨基酸中的一个氨基酸残基或两个或更多个氨基酸残基,所述氨基酸位于与氨基酸中第42,149,153,270和306位氨基酸相对应的位置的氨基酸 来自产生氯霉素的细菌(Amycolatopsis orientalis)的酮还原酶(EvaE)的酸序列被另一个氨基酸残基取代。