公开(公告)号：US20070148777A1

公开(公告)日：2007-06-28

申请号：US11702792

申请日：2007-02-06

申请人： John Gilbert ,  Nghia Chiem

发明人： John Gilbert ,  Nghia Chiem

IPC分类号： G01N37/00

CPC分类号： G01N27/44791 ,  B01D57/02 ,  B01F13/0059 ,  B01F13/0071 ,  B01J19/0093 ,  B01J2219/00371 ,  B01J2219/00378 ,  B01J2219/00783 ,  B01J2219/00831 ,  B01J2219/00837 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00905 ,  B01L3/0244 ,  B01L3/0268 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0439 ,  B01L2400/0487 ,  C12Q1/00 ,  G01N27/447 ,  G01N27/44743 ,  G01N27/44782 ,  G01N2035/1037 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/13 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A fluid interface port in a microfluidic system and a method of forming the fluid interface port is provided. The fluid interface port comprises an opening formed in the side wall of a microchannel sized and dimensioned to form a virtual wall when the microchannel is filled with a first liquid. The fluid interface port is utilized to perform a labeling operation on a sample.

摘要翻译： 提供微流体系统中的流体接口端口和形成流体接口端口的方法。 流体接口端口包括形成在微通道的侧壁中的开口，其尺寸和尺寸被设计成当微通道充满第一液体时形成虚拟壁。 流体接口端口用于对样品执行标签操作。

公开(公告)号：US20060134696A1

公开(公告)日：2006-06-22

申请号：US11181666

申请日：2005-07-13

申请人： Nghia Chiem ,  John Gilbert

发明人： Nghia Chiem ,  John Gilbert

IPC分类号： C40B40/02 ,  C12Q1/48 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/37

CPC分类号： G01N27/44791 ,  B01L3/0244 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2400/0439 ,  C12Q1/00 ,  G01N27/447 ,  G01N2035/1037

摘要： The invention relates to a method of high throughput chemical analysis comprising the steps of combining one test compound with a solution comprising m enzyme(s) and n substrate(s), wherein m is an integer equal to one or greater, n is an integer equal to one or greater, and m+n≧3, incubating for a period of time said test compound within said solution, separating the chemical species in said combined solution by a chromatography step after said incubating step, and measuring the relative amounts of substrates and separately identifiable products produced therefrom by a chemical reaction catalyzed by said enzymes. The present SMSBEA assays are particularly well suited to enzyme-substrate systems in which both the substrate(s) and product(s) have mobilities such that they can be separated on short chromatography columns. The method of the invention is also particularly well suited to HTS applications in which an enzyme agonist or antagonist is sought. An advantage of the method is that the effects of a test compound on several enzymes may be analyzed simultaneously and without substantial purification of the enzyme solution, e.g., whole cell lysates.