公开(公告)号：US20070289881A1

公开(公告)日：2007-12-20

申请号：US11849790

申请日：2007-09-04

申请人： Nigel Forrow ,  Gurdial Sanghera ,  Jared Watkin ,  Stephen Walters

发明人： Nigel Forrow ,  Gurdial Sanghera ,  Jared Watkin ,  Stephen Walters

IPC分类号： G01N27/26 ,  C12M1/34

CPC分类号： C12Q1/006 ,  C12Q1/004 ,  C12Q1/005 ,  G01N27/3272 ,  Y10T29/49155

摘要： The present invention is based on the discovery of NAD+ and NADP+ mediator compounds that do not bind irreversibly to thiol groups in the active sites of intracellular dehydrogenase enzymes. Such mediator compounds avoid a common mode of enzyme inhibition. The mediators can therefore increase the stability and reliability of the electrical response in amperometric electrodes constructed from NAD- or NADP-dependent enzymes.

摘要翻译： 本发明是基于不与细胞内脱氢酶的活性位点中的硫醇基团不可逆地结合的NAD +和NADP + +介质化合物的发现。 这种介体化合物避免了酶抑制的共同模式。 因此，介体可以增加由NAD-或NADP依赖性酶构建的电流电极的电响应的稳定性和可靠性。

公开(公告)号：US20060201805A1

公开(公告)日：2006-09-14

申请号：US11420628

申请日：2006-05-26

申请人： Nigel Forrow ,  Shridhara Karinka

发明人： Nigel Forrow ,  Shridhara Karinka

IPC分类号： C12Q1/00 ,  C12M1/00

CPC分类号： C12Q1/006 ,  C12Q1/004

摘要： The present invention provides reagent compositions, and analyte measuring devices and methods that utilize the reagent compositions.

摘要翻译： 本发明提供了试剂组合物以及利用试剂组合物的分析物测量装置和方法。

公开(公告)号：US20080023326A1

公开(公告)日：2008-01-31

申请号：US11839037

申请日：2007-08-15

申请人： Nigel Forrow ,  Xiang Zhang ,  Catherine McTigue

发明人： Nigel Forrow ,  Xiang Zhang ,  Catherine McTigue

IPC分类号： C12Q1/25 ,  G01N27/416 ,  G01N33/66

CPC分类号： C12Q1/001 ,  G01N33/5438 ,  Y10T156/10

摘要： A biosensor for determining the concentration of an analyte in a biological sample. The biosensor comprises a support, a reference electrode or a counter electrode or both disposed on the support, a working electrode disposed on the support, the working electrode spaced apart from the other electrode or electrodes on the support, a covering layer defining a sample chamber over the electrodes, an aperture in the covering layer for receiving a sample, and at least one layer of mesh in the sample chamber between the covering layer and the electrodes. The at least one layer of mesh has coated thereon a silicone surfactant. Certain silicone surfactants are as effective as fluorinated surfactants with respect to performance of biosensors. These surfactants, when coated onto the mesh layer of the biosensor, are effective in facilitating the transport of aqueous test samples, such as blood, in the sample chamber.

公开(公告)号：US5710011A

公开(公告)日：1998-01-20

申请号：US400133

申请日：1995-03-06

申请人： Nigel Forrow ,  Stephen Walters

发明人： Nigel Forrow ,  Stephen Walters

IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/566

CPC分类号： C12Q1/26 ,  C12Q1/004 ,  Y10S435/817 ,  Y10S436/801

摘要： Half-sandwich complexes of transition metals having a single .pi.-ligand with delocalised .pi. bonding are of use in mediating electron transfer between an electrode and a redox protein, for example an oxidoreductase enzyme such as in an amperometric enzyme sensor. Suitable compounds include complexes of the general formula: (.pi.-ligand)M(not-.pi.-ligand).sub.n wherein the complex is charged or neutral, .pi.-ligand represents the single .pi.-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not .pi.-ligands, and the n not-.pi.-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transitional metal M.

摘要翻译： 具有单一pi配体和具有离域pI键的过渡金属的半夹心复合物可用于介导电极和氧化还原蛋白之间的电子转移，例如氧化还原酶，例如电流型酶传感器。 合适的化合物包括通式（pi -ligand）M（不对称）n的络合物，其中络合物带电或中性，π-配体代表单个π键配体，M代表过渡金属原子，n 是不是π-配体的配体的数量，并且n个不同的配位相同或不同，是一价或多价的，并且用于满足过渡金属M的化合价。