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    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes
    2.
    发明申请
    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes 审中-公开
    用二苄唑胺染料标记多核苷酸的方法

    公开(公告)号：US20070099210A1

    公开(公告)日：2007-05-03

    申请号：US11466085

    申请日：2006-08-21

    Applicant: Scott Benson ,  Joe Lam ,  Steven Menchen

    Inventor： Scott Benson ,  Joe Lam ,  Steven Menchen

    IPC: C12Q1/68 ,  C07H21/04 ,  C07D491/22

    CPC classification number: C09B11/24 ,  C07D209/60 ,  C07D221/10 ,  C07D311/78 ,  C07D491/14 ,  C07D491/147 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C09B11/02 ,  C12Q1/6869 ,  Y10T436/143333 ,  C12Q2563/107 ,  C12Q2527/125

    Abstract: Dibenzorhodamine compounds having the structure are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.

    Abstract translation: 公开了具有该结构的二苄氧苯胺类化合物，包括其氮和芳基取代的形式。 此外，公开了可用于合成这些化合物的两种中间体，具有包括其氮和芳基取代形式的结构的第一中间体和具有其氮和芳基取代形式的结构的第二中间体，其中位置 C14至C18分别选自氢，氯，氟，低级烷基，羧酸，磺酸，-CH 2 OH，烷氧基，苯氧基，连接基团及其取代形式 。 本发明还包括包含二苄基罗丹胺化合物的能量转移染料，用二苯并呋喃胺化合物标记的核苷，以及使用二苯并呋喃胺化合物的核酸分析方法。

    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes
    4.
    发明申请
    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes 有权

    公开(公告)号：US20060063186A1

    公开(公告)日：2006-03-23

    申请号：US11226069

    申请日：2005-09-14

    Applicant: Scott Benson ,  Steven Menchen ,  Krishna Upadhya

    Inventor： Scott Benson ,  Steven Menchen ,  Krishna Upadhya

    IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/04

    CPC classification number: C07C245/08 ,  C07C50/18 ,  C07D311/90 ,  C07H21/00 ,  C07H21/04 ,  C09B6/00 ,  C09B11/08 ,  C09B33/02 ,  C09B35/021 ,  C09B35/50 ,  G01N33/542

    Abstract: Dark quencher constructs, termed “multi-chromophoric quenchers” are described herein that comprise at least two dark quenching moieties, which can be the same or different, linked together by at least one multivalent linking moiety. The structure of the multi-chromophoric quenchers can be varied to selectively enhance quenching within a specific range of reporter emission wavelengths. This can be accomplished by linking together, into a single molecule, two or more identical quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to quench a broader range of reporter emission wavelengths than previously possible. This can be accomplished by linking together, into a single molecule, two or more different quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to simultaneously broaden the absorption range and increase the total absorption within the absorption range. This can be done by combining the two concepts described above. In other words, multiple types of quenching moieties can be employed to increase the absorption range and a multiple number of each type of quenching moiety can be used to increase the total absorptivity within the absorption range. The multi-chromophoric quenchers can be tethered to probes for biomolecules, insoluble supports and/or fluorescent dyes for use in a wide variety of biomolecular assays.

    PROPARGYLETHOXYAMINO NUCLEOTIDES
    6.
    发明申请
    PROPARGYLETHOXYAMINO NUCLEOTIDES 审中-公开
    丙基胆碱胺核苷酸

    公开(公告)号：US20060286561A1

    公开(公告)日：2006-12-21

    申请号：US11277481

    申请日：2006-03-24

    Applicant: Shaheer Khan ,  Steven Menchen ,  Barnett Rosenblum

    Inventor： Shaheer Khan ,  Steven Menchen ,  Barnett Rosenblum

    IPC: C12Q1/68 ,  C07H19/04 ,  C12P19/34 ,  C07H21/04

    CPC classification number: C07H21/00 ,  C07H19/04 ,  C07H21/04 ,  C12Q1/686

    Abstract: Propargylethoxyamino nucleosides are disclosed having the structure wherein R1 and R2 are —H lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is —H or —H; W2 is —H or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3′-position; and W3 is —PO4, —P2O7, —P3O10, phosphate analog, or —OH. Additionaly, a primer extension method is provided employing the above propargylethoxyamino nucleosides.

    Abstract translation: 公开了具有其中R 1和R 2为-H低级烷基或标记的结构的丙炔乙氧基氨基核苷; B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基; W 1是-H或-H; W 2是-H或使核苷不能在3'-位形成磷酸二酯键的部分; 和W 3是-PO 4，-P 2 O 7，-P 3， 或磷酸酯类似物或-OH。 另外，使用上述炔丙氧基氨基核苷提供引物延伸方法。

    Mobility-modifying cyanine dyes
    7.
    发明申请
    Mobility-modifying cyanine dyes 审中-公开
    迁移性改性花青染料

    公开(公告)号：US20050107617A1

    公开(公告)日：2005-05-19

    申请号：US10801092

    申请日：2004-03-15

    Applicant: Steven Menchen ,  Scott Benson ,  Barnett Rosenblum ,  Shaheer Khan

    Inventor： Steven Menchen ,  Scott Benson ,  Barnett Rosenblum ,  Shaheer Khan

    IPC: C09B23/00 ,  C07F9/24 ,  C07H21/00 ,  C09B1/00 ,  C09B11/28 ,  C09B23/02 ,  G01N27/447 ,  G01N33/52 ,  G01N33/533 ,  C07D417/02 ,  C07D413/02 ,  C07D43/02

    CPC classification number: C07H21/00 ,  C07F9/2408 ,  C09B23/02 ,  G01N27/44726

    Abstract: The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the hetercyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence-based 4-color nucleic acid sequencing reactions.

    Abstract translation: 本发明提供了一类新颖的荧光花青染料化合物，其在一个杂环氮原子上被修饰，具有迁移率调节部分，其允许调节或调谐用迁移率调节花青染料标记的多核苷酸的电泳迁移率 一种可预测的方式，同时保留酶活性。 可预测地调节染料的相对电泳迁移率的能力允许为各种应用（包括基于荧光的4色核酸测序反应）创建各种结构的迁移率匹配荧光染料组。

    Methods and kits for methylation detection
    10.
    发明申请
    Methods and kits for methylation detection 有权
    用于甲基化检测的方法和试剂盒

    公开(公告)号：US20050266458A1

    公开(公告)日：2005-12-01

    申请号：US11119985

    申请日：2005-05-02

    Applicant: Mark Andersen ,  Jer-Kang Chen ,  Michael Hunkapiller ,  Steven Menchen

    Inventor： Mark Andersen ,  Jer-Kang Chen ,  Michael Hunkapiller ,  Steven Menchen

    IPC: C12Q1/68

    CPC classification number: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6862 ,  C12Q2537/164 ,  C12Q2521/501

    Abstract: Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.

    Abstract translation: 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。

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