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8 条记录 (耗时 0.093 秒)

    Methods of modulating cell attachment and migration
    4.
    发明授权
    Methods of modulating cell attachment and migration 失效
    调节细胞附着和迁移的方法

    公开(公告)号：US06566074B1

    公开(公告)日：2003-05-20

    申请号：US09525649

    申请日：2000-03-15

    申请人： Paul F. Goetinck

    发明人： Paul F. Goetinck

    IPC分类号： G01N3353

    CPC分类号： C07K16/2896 ,  A61K38/39 ,  A61K2039/505 ,  C07K14/78 ,  C07K2317/70

    摘要： The invention features a method of modulating, e.g., inhibiting or promoting, the spatial or positional relationship of a cell to a substrate, or modulating the intracellular response of a cell to a substrate, in vitro or in vivo. The method includes administering an agent which modulates the interaction, e.g., the binding, of the syndecan-4 ectodomain with a counterligand, thereby modulating the spatial or positional relationship of a cell to a substrate, or modulating the intracellular response of a cell to a substrate. The preferred counterligand is an ECM component, e.g., the heparin-binding domain of a component of the extracellular matrix (ECM) such as fibronectin, vitronectin, a laminin or a collagen. The invention also features methods of identifying compounds which modulate, e.g., inhibit or promote, the spatial or positional relationship of a cell to a substrate, or modulate the intracellular response of a cell to a substrate, and methods of treating a subject having a disorder characterized by unwanted or abnormal cell adhesion or spreading, e.g., cancer.

    摘要翻译： 本发明的特征在于在体外或体内调节，例如抑制或促进细胞与底物的空间或位置关系或调节细胞对底物的细胞内应答的方法。 该方法包括施用调节syndecan-4胞外域与抗衡配体的相互作用（例如，结合）的试剂，从而调节细胞与底物的空间或位置关系，或调节细胞对细胞内的反应 基质。 优选的反配对是ECM组分，例如细胞外基质（ECM）的组分的肝素结合结构域，例如纤连蛋白，玻连蛋白，层粘连蛋白或胶原。 本发明还涉及鉴定化合物的方法，所述化合物调节，例如抑制或促进细胞与底物的空间或位置关系，或调节细胞对底物的细胞内应答，以及治疗患有疾病的受试者的方法 其特征在于不想要的或异常的细胞粘附或扩散，例如癌症。

    Syndecan-4 binding protein (S4BP) and uses thereof
    6.
    发明授权
    Syndecan-4 binding protein (S4BP) and uses thereof 失效
    Syndecan-4结合蛋白（S4BP）及其用途

    公开(公告)号：US06566489B1

    公开(公告)日：2003-05-20

    申请号：US09526400

    申请日：2000-03-15

    申请人： Paul F. Goetinck ,  Peter C. Baciu

    发明人： Paul F. Goetinck ,  Peter C. Baciu

    IPC分类号： C07K1400

    CPC分类号： C07K14/47 ,  A61K48/00 ,  C07K14/575

    摘要： The present invention relates to a gene encoding a novel protein, syndecan-4 binding protein (S4BP), which binds to syndecan-4. S4BP polypeptides play a role in cell matrix interactions. Described herein are isolated and antisense nucleic acids molecules, recombinant expression vectors, host cells and non-human transgenic animals containing an insertion or a disruption of the S4BP gene. Diagnostic, screening and therapeutic methods utilizing the compositions of the invention are also provided.

    摘要翻译： 本发明涉及编码与syndecan-4结合的新型蛋白质，syndecan-4结合蛋白（S4BP）的基因。 S4BP多肽在细胞基质相互作用中发挥作用。 本文描述了分离和反义的核酸分子，重组表达载体，宿主细胞和含有S4BP基因插入或破坏的非人转基因动物。 还提供了利用本发明组合物的诊断，筛选和治疗方法。

    Fusion polypeptide containing fragments of cartilage matrix protein and link protein
    7.
    发明授权
    Fusion polypeptide containing fragments of cartilage matrix protein and link protein 失效
    融合多肽含有软骨基质蛋白和链接蛋白的片段

    公开(公告)号：US5986052A

    公开(公告)日：1999-11-16

    申请号：US463218

    申请日：1995-06-05

    申请人： Paul F. Goetinck ,  M. Mehrdad Tondravi ,  Francois Binette

    发明人： Paul F. Goetinck ,  M. Mehrdad Tondravi ,  Francois Binette

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K48/00 ,  A61P3/00 ,  A61P43/00 ,  C07K14/47 ,  C07K14/78 ,  C07K19/00 ,  C12P21/02 ,  C07K1/00 ,  C07K17/02 ,  C12N11/02 ,  C12N15/00

    CPC分类号： C07K14/47 ,  C07K14/78 ,  A61K38/00 ,  C07K2319/33 ,  Y10S530/81 ,  Y10S530/815

    摘要： Link protein and cartilage matrix protein, which are two major components of the extracellular cartilage matrix, have been found to bind to each other. Polypeptide fragments of cartilage matrix protein and link protein are produced. A recombinant fusion polypeptide is prepared containing a fragment of cartilage matrix protein that binds to link protein and a fragment of link protein that binds to cartilage matrix protein. The cartilage matrix protein fragment may bind to collagen and contain the CMP-1 or CMP-2 domain, and the link protein may bind to a complex of hyaluronic acid and proteoglycan. The fragments or fusion polypeptide can be administered for repair of diseased or injured cartilaginous and non-cartilaginous tissue by promoting binding of a complex of proteoglycan and hyaluronic acid to collagen. The fragments or fusion polypeptide can be anchored to the surface of a prosthetic device, implant or tissue graft to promote adherence of tissue and biocompatibility. By anchoring a fragment of cartilage matrix protein or link protein on a surface, cartilaginous tissue, cartilage matrix protein or link protein may be attached to the surface.

    摘要翻译： 已经发现作为细胞外软骨基质的两个主要成分的链接蛋白和软骨基质蛋白彼此结合。 产生软骨基质蛋白和连锁蛋白的多肽片段。 制备包含结合链节蛋白的软骨基质蛋白片段和结合软骨基质蛋白的连接蛋白片段的重组融合多肽。 软骨基质蛋白片段可能与胶原结合并含有CMP-1或CMP-2结构域，并且连接蛋白可能与透明质酸和蛋白聚糖复合物结合。 可以通过促进蛋白多糖和透明质酸的复合物与胶原的结合来施用片段或融合多肽来修复患病或损伤的软骨和非软骨组织。 片段或融合多肽可以锚定到假体装置，植入物或组织移植物的表面以促进组织的粘附和生物相容性。 通过将软骨基质蛋白或连接蛋白的片段锚定在表面上，可以将软骨组织，软骨基质蛋白或连接蛋白附着于表面。

    Method for attaching cartilaginous tissue, cartilage matrix protein or link protein to a surface
    8.
    发明授权
    Method for attaching cartilaginous tissue, cartilage matrix protein or link protein to a surface 失效
    将软骨组织，软骨基质蛋白或连接蛋白附着于表面的方法

    公开(公告)号：US5872094A

    公开(公告)日：1999-02-16

    申请号：US1078

    申请日：1993-01-06

    申请人： Paul F. Goetinck ,  M. Mehrdad Tondravi ,  Francois Binette

    发明人： Paul F. Goetinck ,  M. Mehrdad Tondravi ,  Francois Binette

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K48/00 ,  A61P3/00 ,  A61P43/00 ,  C07K14/47 ,  C07K14/78 ,  C07K19/00 ,  C12P21/02 ,  A61K38/17 ,  C07K14/00 ,  C07K17/08 ,  C12N11/08

    CPC分类号： C07K14/47 ,  C07K14/78 ,  A61K38/00 ,  C07K2319/33 ,  Y10S530/81 ,  Y10S530/815

    摘要： Link protein and cartilage matrix protein, which are two major components of the extracellular cartilage matrix, have been found to bind to each other. Cartilaginous tissue is attached to a surface by anchoring on the surface a fragment of cartilage matrix protein capable of binding to link protein or to collagen and link protein, or a fragment of link protein capable of binding to cartilage matrix protein or to cartilage matrix protein and a complex or proteoglycan and hyaluronic acid, and contacting the surface with cartilaginous tissue. Cartilage matrix protein is attached to a surface by anchoring on the surface a fragment of link protein capable of binding to cartilage matrix protein, and contacting the surface with cartilage matrix protein. Link protein is attached to a surface by anchoring on the surface a fragment of cartilage matrix protein capable of binding to link protein, and contacting the surface with link protein. Human cartilage matrix protein, link protein and fragments thereof are used, and the fragments may be recombinantly made. A recombinant fusion polypeptide is prepared that contains the fragments of cartilage matrix protein and link protein. The fragments or fusion polypeptide can be administered for repair of diseased or injured cartilaginous and non-cartilaginous tissue by promoting the binding of a complex of proteoglycan and hyaluronic acid to collagen. The fragments or fusion polypeptide can also be anchored to the surface of a prosthetic device, implant or tissue graft to promote adherence of tissue and biocompatibility.

    摘要翻译： 已经发现作为细胞外软骨基质的两个主要成分的链接蛋白和软骨基质蛋白彼此结合。 软骨组织通过在表面上锚定能够结合链节蛋白或胶原蛋白和连接蛋白的软骨基质蛋白的片段或能够结合软骨基质蛋白或软骨基质蛋白的连接蛋白的片段而附着于表面， 复合物或蛋白聚糖和透明质酸，并使表面与软骨组织接触。 软骨基质蛋白通过在表面上锚定能够结合软骨基质蛋白的连接蛋白片段，并使表面与软骨基质蛋白接触来附着于表面。 链接蛋白通过在表面上锚定能够结合链接蛋白的软骨基质蛋白的片段，并使表面与链接蛋白接触来附着于表面。 使用人软骨基质蛋白，连接蛋白及其片段，并且重组制备片段。 制备含有软骨基质蛋白和连接蛋白片段的重组融合多肽。 可以通过促进蛋白多糖和透明质酸的复合物与胶原的结合来施用片段或融合多肽来修复患病或损伤的软骨和非软骨组织。 片段或融合多肽还可以锚定到假体装置，植入物或组织移植物的表面以促进组织的粘附和生物相容性。