公开(公告)号：US08338153B2

公开(公告)日：2012-12-25

申请号：US12825510

申请日：2010-06-29

申请人： Peter Stougaard ,  Ole Cai Hansen

发明人： Peter Stougaard ,  Ole Cai Hansen

IPC分类号： C12N9/04 ,  A61K38/44 ,  A61K47/00 ,  A61K8/00 ,  A21D2/00 ,  A21D8/04 ,  A21D13/00 ,  A23C9/00 ,  A23K1/165 ,  A23L2/00

CPC分类号： C12P21/02 ,  C12N9/0006

摘要： A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.

摘要翻译： 通过重组DNA技术生产己糖氧化酶的方法，重组己糖氧化酶以及这种酶的使用，特别是在制造面团和乳制品，动物饲料，药物，化妆品，牙科护理产品等食品的制造中 的内酯。 编码酶的DNA的合适来源是海藻类物种，包括软骨including，，鸢尾虱和uthor and。 在有用的实施方案中，重组己糖氧化酶由巴斯德毕赤酵母，酿酒酵母或大肠杆菌产生。

公开(公告)号：US20090275103A1

公开(公告)日：2009-11-05

申请号：US12396954

申请日：2009-03-03

申请人： Peter Stougaard ,  Ole Cai Hansen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

发明人： Peter Stougaard ,  Ole Cai Hansen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

IPC分类号： C12N9/04

CPC分类号： C12N9/0006 ,  A01N63/00

摘要： Use of a preparation as an antimicrobial agent or as an anti-oxidant; wherein said preparation comprises a polypeptide having hexose oxidase activity, the polypeptide being in a substantially pure form, and said polypeptide comprises at least one amino acid sequence selected from the group consisting of (i) (SEQ ID NO: 1) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro (ii) (SEQ ID NO: 2) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly- Tyr-Asp-X-X-X-Gly-Tyr-X-Val-Ser-Ser- (iii) (SEQ ID NO: 3) Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser- Asn-Leu-X-Phe- (iv) (SEQ ID NO: 4) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His- Thr (v) (SEQ ID NO: 5) Tyr-Tyr-Phe-Lys (vi) (SEQ ID NO: 6) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly- Thr-X-Asp (vii) (SEQ ID NO: 7) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp (viii) (SEQ ID NO: 8) X-Ile-Arg-Asp-Phe-Tyr-Glu-Glu-Met where X represents an amino acid selected from the group consisting of Ala, Arg, Asn, Asp, Asx, Cys, Gln, Glu, Glx, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr and Val.

摘要翻译： 使用制剂作为抗微生物剂或作为抗氧化剂; 其中所述制剂包含具有己糖氧化酶活性的多肽，所述多肽为基本上纯的形式，所述多肽包含至少一个选自下组的氨基酸序列：（i）（SEQ ID NO：1）Tyr-Glu- Pro-Tyr-Gly-Gly-Val-Pro（ii）（SEQ ID NO：2）Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly-Tyr-Asp-XXX -Gly-Tyr-X-Val-Ser-Ser-（iii）（SEQ ID NO：3）Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser-Asn-Leu -X-Phe-（iv）（SEQ ID NO：4）Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His-Thr（v）（SEQ ID NO： 5）Tyr-Tyr-Phe-Lys（vi）（SEQ ID NO：6）Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly-Thr-X-Asp ）（SEQ ID NO：7）Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp（viii）（SEQ ID NO：8）X-Ile-Arg-Asp-Phe-Tyr -Glu-Glu-Met，其中X表示选自Ala，Arg，Asn，Asp，Asx，Cys，Gln，Glu，Glx，Gly，His，Ile，Leu，Lys，Met，Phe， Pro，Ser，Thr，Trp，Tyr和Val。

公开(公告)号：US08288143B2

公开(公告)日：2012-10-16

申请号：US13148853

申请日：2010-02-09

申请人： Peter Stougaard ,  Mariane Schmidt

发明人： Peter Stougaard ,  Mariane Schmidt

IPC分类号： C07K1/00 ,  C12N9/38 ,  C07H21/04 ,  C12N15/00 ,  C12N1/20

CPC分类号： A23C9/1206 ,  A23C9/123 ,  A23C19/032 ,  C12N9/2471 ,  C12Y302/01023

摘要： A novel cold-active beta-galactosidase is enzyme specific for lactose. The enzyme is thus useful in e.g. the food industry for catalyzing at low temperatures the hydrolysis of lactose disaccharide into its constituent monosaccharides, glucose and galactose. A method produces the cold-active beta-galactosidase by recombinant DNA technology.

摘要翻译： 一种新型的冷活性β-半乳糖苷酶是对乳糖特异的酶。 因此，该酶可用于例如。 食品工业在低温下催化乳糖二糖水解成其组成单糖，葡萄糖和半乳糖。 一种方法通过重组DNA技术产生冷活性β-半乳糖苷酶。

公开(公告)号：US08178090B2

公开(公告)日：2012-05-15

申请号：US12396954

申请日：2009-03-03

申请人： Peter Stougaard ,  Ole Cai Hansen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

发明人： Peter Stougaard ,  Ole Cai Hansen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

IPC分类号： A61K38/44 ,  A61K8/00 ,  A61K8/66 ,  C12N9/04 ,  A23K1/165 ,  A21D8/04 ,  C12N15/53 ,  C12N15/70 ,  C12N15/80

CPC分类号： C12N9/0006 ,  A01N63/00

摘要： Use of a preparation as an antimicrobial agent or as an anti-oxidant; wherein said preparation comprises a polypeptide having hexose oxidase activity, the polypeptide being in a substantially pure form, and said polypeptide comprises at least one amino acid sequence selected from the group consisting of (i)(SEQ ID NO: 1) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro (ii)(SEQ ID NO: 2) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly- Tyr-Asp-X-X-X-Gly-Tyr-X-Val-Ser-Ser- (iii)(SEQ ID NO: 3) Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser- Asn-Leu-X-Phe- (iv)(SEQ ID NO: 4) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His- Thr (v)(SEQ ID NO: 5) Tyr-Tyr-Phe-Lys (vi)(SEQ ID NO: 6) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly- Thr-X-Asp (vii)(SEQ ID NO: 7) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp (viii)(SEQ ID NO: 8) X-Ile-Arg-Asp-Phe-Tyr-Glu-Glu-Met where X represents an amino acid selected from the group consisting of Ala, Arg, Asn, Asp, Asx, Cys, Gln, Glu, Glx, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr and Val.

摘要翻译： 使用制剂作为抗微生物剂或作为抗氧化剂; 其中所述制剂包含具有己糖氧化酶活性的多肽，所述多肽为基本上纯的形式，所述多肽包含至少一个选自下组的氨基酸序列：（i）（SEQ ID NO：1）Tyr-Glu- Pro-Tyr-Gly-Gly-Val-Pro（ii）（SEQ ID NO：2）Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly-Tyr-Asp-XXX -Gly-Tyr-X-Val-Ser-Ser-（iii）（SEQ ID NO：3）Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser-Asn-Leu -X-Phe-（iv）（SEQ ID NO：4）Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His-Thr（v）（SEQ ID NO： 5）Tyr-Tyr-Phe-Lys（vi）（SEQ ID NO：6）Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly-Thr-X-Asp ）（SEQ ID NO：7）Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp（viii）（SEQ ID NO：8）X-Ile-Arg-Asp-Phe-Tyr -Glu-Glu-Met，其中X表示选自Ala，Arg，Asn，Asp，Asx，Cys，Gln，Glu，Glx，Gly，His，Ile，Leu，Lys，Met，Phe， Pro，Ser，Thr， Trp，Tyr和Val。

公开(公告)号：US06924366B2

公开(公告)日：2005-08-02

申请号：US09824053

申请日：2001-04-03

申请人： Peter Stougaard ,  Ole Cai Hansen

发明人： Peter Stougaard ,  Ole Cai Hansen

IPC分类号： A23K1/165 ,  A21D8/04 ,  A23K3/03 ,  A23L1/23 ,  A23L3/3526 ,  A23L3/3571 ,  A61K8/66 ,  A61Q11/00 ,  C07H21/04 ,  C12N1/19 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/00 ,  C12N15/09 ,  C12N15/53 ,  C12P17/02 ,  C12P21/02 ,  C12P21/06 ,  C12R1/19 ,  C12R1/84 ,  C12R1/865 ,  C12R1/89 ,  A21D2/00 ,  C12N15/74 ,  C12N15/79

CPC分类号： C12N9/0006 ,  A21D8/04 ,  A23K30/18 ,  A23L3/3571 ,  A23L27/24 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  C12P17/02 ,  C12P21/02

摘要： A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.

公开(公告)号：US20110202950A1

公开(公告)日：2011-08-18

申请号：US12705744

申请日：2010-02-15

申请人： Peter STOUGAARD

发明人： Peter STOUGAARD

IPC分类号： H04N7/16

CPC分类号： H04N7/17318 ,  G06Q30/02 ,  H04N21/4758 ,  H04N21/4782 ,  H04N21/4784 ,  H04N21/812

摘要： A system, and methods for using that system, are provided that encourage viewers to watch and listen to a variety of multimedia event presentations. The various multimedia event presentations are received by the viewers on a receiving module and are displayed on a display module. Along with the multimedia event presentations (primary in the form of broadcast shows and ancillary in the form of advertisements and commercials), the viewers are provided with one or more interactive presentations (games) and additional browsing features by means of graphics-rich overlays through which they can participate and interact. Interaction is provided for by way of user input (communications) modules connected to the receiving modules and the display modules.

摘要翻译： 提供了系统和使用该系统的方法，其鼓励观看者观看并听取各种多媒体事件演示。 各种多媒体事件呈现由接收模块上的观众接收并显示在显示模块上。 随着多媒体活动演示（主要以广播节目形式和辅助形式的广告和广告），观众通过富有图形的重叠通过一个或多个交互式演示（游戏）和附加的浏览功能 他们可以参与和互动。 通过连接到接收模块和显示模块的用户输入（通信）模块提供交互。

公开(公告)号：US20120058223A1

公开(公告)日：2012-03-08

申请号：US13148853

申请日：2010-02-09

申请人： Peter Stougaard ,  Mariane Schmidt

发明人： Peter Stougaard ,  Mariane Schmidt

IPC分类号： C12N9/38 ,  C12N15/63 ,  C12N1/21 ,  A23C19/00 ,  C12N1/15 ,  A23C7/00 ,  C12N9/96 ,  A23C9/12 ,  C12N15/56 ,  C12N1/19

CPC分类号： A23C9/1206 ,  A23C9/123 ,  A23C19/032 ,  C12N9/2471 ,  C12Y302/01023

摘要： A novel cold-active beta-galactosidase is enzyme specific for lactose. The enzyme is thus useful in e.g. the food industry for catalyzing at low temperatures the hydrolysis of lactose disaccharide into its constituent monosaccharides, glucose and galactose. A method produces the cold-active beta-galactosidase by recombinant DNA technology.

摘要翻译： 一种新型的冷活性β-半乳糖苷酶是对乳糖特异的酶。 因此，该酶可用于例如。 食品工业在低温下催化乳糖二糖水解成其组成单糖，葡萄糖和半乳糖。 一种方法通过重组DNA技术产生冷活性β-半乳糖苷酶。

公开(公告)号：US07544795B2

公开(公告)日：2009-06-09

申请号：US10919492

申请日：2004-08-17

申请人： Peter Stougaard ,  Ole Cai Hansen

发明人： Peter Stougaard ,  Ole Cai Hansen

IPC分类号： C12N15/53 ,  C12N15/62 ,  C12N15/70 ,  C12N15/79 ,  C12N9/04 ,  A21D2/00

CPC分类号： C12N9/0006 ,  A21D8/04 ,  A23K30/18 ,  A23L3/3571 ,  A23L27/24 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  C12P17/02 ,  C12P21/02

摘要： A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.

公开(公告)号：US20090041899A1

公开(公告)日：2009-02-12

申请号：US11979134

申请日：2007-10-31

申请人： Peter Stougaard ,  Ole Cai Hansen

发明人： Peter Stougaard ,  Ole Cai Hansen

IPC分类号： A23K1/165 ,  C12N9/04 ,  A21D2/08 ,  C12N1/16

CPC分类号： C12N9/0006 ,  A21D8/04 ,  A23K30/18 ,  A23L3/3571 ,  A23L27/24 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  C12P17/02 ,  C12P21/02

摘要： A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.

公开(公告)号：US06251626B1

公开(公告)日：2001-06-26

申请号：US08669304

申请日：1996-09-11

申请人： Peter Stougaard ,  Ole Cai Hansen

发明人： Peter Stougaard ,  Ole Cai Hansen

IPC分类号： C12N904

CPC分类号： C12N9/0006 ,  A21D8/04 ,  A23K30/18 ,  A23L3/3571 ,  A23L27/24 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  C12P17/02 ,  C12P21/02

摘要： A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.

摘要翻译： 通过重组DNA技术生产己糖氧化酶的方法，重组己糖氧化酶以及这种酶的使用，特别是在制造面团和乳制品，动物饲料，药物，化妆品，牙科护理产品等食品的制造中 的内酯。 编码酶的DNA的合适来源是海藻类物种，包括软骨including，，鸢尾虱和uthor and。 在有用的实施方案中，重组己糖氧化酶由巴斯德毕赤酵母，酿酒酵母或大肠杆菌产生。