公开(公告)号：US20250067705A1

公开(公告)日：2025-02-27

申请号：US18294759

申请日：2022-06-13

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Young-Ha Hwang ,  Henry Julian Hinton ,  Han Sae Jung ,  Woo-Bin Jung

IPC: G01N27/416

Abstract: An electrochemical apparatus includes an array of pixels disposed on a chip, stimulator circuitry disposed on the chip and configured to provide electrical input signals to cause stimulation of the pixels of the array, and sensor circuitry disposed on the chip and configured to read electrical output signals from the pixels of the array. The stimulator circuitry is configured to provide the input signals to cause stimulation of the pixels individually, and the sensor circuitry is configured to selectively read the output signals from the pixels while the pixels are being stimulated. The sensor circuitry is configured to measure an open-circuit voltage at each of the pixels and a current flow at each of the pixels while the pixels are being stimulated by the stimulator circuitry. The open-circuit voltage may be measured while the current flow is being measured.

公开(公告)号：US20250066842A1

公开(公告)日：2025-02-27

申请号：US18814007

申请日：2024-08-23

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Pardis Sabeti ,  Cameron Myhrvold ,  Sameed Siddiqui

IPC: C12Q1/682 ,  C12N9/02 ,  C12N9/22 ,  C12N15/11

Abstract: Cas-cleavable reporter systems, CRISPR-Cas systems thereof, and methods of use thereof in CRISPR-Cas based diagnostics. Cas-cleavable reporter systems may generate a luminescent, fluorescent, or other detectable signal upon Cas-collateral cleavage of one or more reporter system components. CRISPR-Cas systems may comprise a Cas protein having collateral cleavage activity, guide molecules, and the Cas-cleavable reporter system. Cas-cleavable reporter systems may be a split luciferase reporter system, at least one element of which is bound to beads. For multiplexing, CRISPR-Cas systems may comprise a Cas-cleavable quenched reporter system, optionally a Cas-cleavable quenched fluorescent reporter system, a Cas protein having collateral cleavage activity, and target-specific guide molecules attached to color-coded beads. CRISPR-Cas systems may further comprise amplification reagents. Methods may apply said CRISPR-Cas systems to flow cell, well-plate, or other devices, and may measure detectable signals by microscopic, plate reader, or other methods.

公开(公告)号：US20250066765A1

公开(公告)日：2025-02-27

申请号：US18932378

申请日：2024-10-30

Applicant: President and Fellows of Harvard College

Inventor： Xiaowei Zhuang ,  Kok-Hao Chen ,  Alistair Boettiger ,  Jeffrey R. Moffitt ,  Siyuan Wang

IPC: C12N15/10 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6869 ,  G06N7/01 ,  G16B25/00 ,  G16B25/20 ,  G16B30/00 ,  G16B40/00 ,  G16B40/10 ,  G16C20/10

Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

公开(公告)号：US20250064605A1

公开(公告)日：2025-02-27

申请号：US18936638

申请日：2024-11-04

Applicant: President and Fellows of Harvard College

Inventor： Kevin Galloway ,  Conor Walsh ,  Donal Holland ,  Panagiotis Polygerinos ,  Tyler Clites ,  Paxton Maeder-York ,  Ryan Neff ,  Emily Marie Boggs ,  Zivthan Dubrovsky

IPC: A61F2/68 ,  A61B17/00 ,  A61B17/02 ,  A61B17/11 ,  A61B34/00 ,  A61B90/00 ,  A61F2/70 ,  A61F2/72 ,  A61F2/74 ,  A61F5/01 ,  A61H1/02 ,  A61H3/00 ,  B25J9/16 ,  B25J13/00 ,  F15B15/10

Abstract: A wearable device includes at least one soft actuator body incorporated into the device and defining a chamber. A fluid connector is positioned and configured to provide a path for fluid flow into and out of the chamber. A control system is connected with the soft actuator body and comprises a pneumatic or hydraulic pump, valves, and a microcontroller and is configured to control fluid flow through the fluid connector into and out of the chamber to produce at least one actuation motion selected from bending, extending, stiffening, expansion, contraction, twisting, and combinations thereof in the soft actuator body. At least one sensor, including a motion sensor, is incorporated into the device and is in communication with the microcontroller, which is configured to control the fluid flow based on communications from the at least one sensor, including the motion sensor, and to thereby control actuation of the soft actuator body.

公开(公告)号：US20250043349A1

公开(公告)日：2025-02-06

申请号：US18714885

申请日：2022-12-02

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Sandeep DATTA ,  Tatsuya TSUKAHARA ,  David BRANN

IPC: C12Q1/6883 ,  G01N33/50

Abstract: Scents are perceived by the olfactory sensory neurons (OSNs) that line the upper nasal cavity. Each OSN expresses one odorant receptor, and these odorant receptors contact the scent molecules. Methods for determining which odorant receptor(s) are activated by a scent are lacking, making it difficult to replicate or improve scents. The technology as disclosed herein refers to methods relating to activating odor response genes found in olfactory sensory neurons after the neurons are exposed to at least one volatilized chemical compound.

公开(公告)号：US12215326B2

公开(公告)日：2025-02-04

申请号：US15632067

申请日：2017-06-23

Applicant: The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

Inventor： Feng Zhang ,  Patrick Hsu ,  Jonathan S. Gootenberg ,  Aaron Smargon

IPC: C12N15/63 ,  C12N9/22 ,  C12N15/11 ,  C12N15/115 ,  C12N15/82 ,  C12N15/86

Abstract: The invention provides for systems, methods, and compositions for targeting RNA. In particular, the invention provides a non-naturally occurring or engineered RNA-targeting system comprising an RNA-targeting Cas protein and at least one RNA-targeting guide RNA, wherein said RNA-targeting guide RNA is capable of hybridizing with a target RNA in a cell.

公开(公告)号：US20250034759A1

公开(公告)日：2025-01-30

申请号：US18910120

申请日：2024-10-09

Applicant: President and Fellows of Harvard College

Inventor： Qihan Liu ,  Kevin Kit Parker ,  Huibin Chang

IPC: D01D5/14 ,  D01D5/18

Abstract: Systems and methods for focused direction deposition of a micron or nanometer dimension polymeric fiber and materials of such fibers are described herein. Systems and methods employ one or more gas flows to entrain and deflect fibers produced by a rotary jet spinning system forming a focused fiber stream. Some embodiments enable control of alignment and distribution of the fibers with a relatively high fiber throughput.

公开(公告)号：US20250034549A1

公开(公告)日：2025-01-30

申请号：US18715578

申请日：2022-12-02

Applicant: The Broad Institute, Inc. ,  President and Fellows of Harvard College

Inventor： David R. Liu ,  Thomas J. Cahill, III ,  Philip DeSouza ,  Aditya Raguram ,  Samagya Banskota ,  Meirui An

IPC: C12N15/10 ,  C07K14/005 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86

Abstract: Disclosed herein are compositions, methods, kits, and systems relating to efficient delivery of cargos (e.g., therapeutic cargos) into cells, for instance, for in vivo delivery. The present disclosure provides lipid-containing particles (e.g., virus-like particles) for delivering therapeutic cargos. The present disclosure also provides polynucleotides encoding the lipid-containing particles provided herein, which may be useful for producing said lipid-containing particles. Also provided are methods for editing nucleic acid molecules in cells using the lipid-containing particles provided herein, as well as cells and kits comprising the lipid-containing particles.

公开(公告)号：US20250032605A1

公开(公告)日：2025-01-30

申请号：US18715905

申请日：2022-12-05

Applicant: The General Hospital Corporation ,  President and Fellows of Harvard College

Inventor： Aaron G. SCHMIDT ,  Blake M. HAUSER ,  Timothy M. CARADONNA

IPC: A61K39/215 ,  A61K39/00 ,  A61P31/14 ,  C07K14/005 ,  C12N7/00

Abstract: The invention relates to a monomeric immunogen comprising one receptor binding domain of a Coronavirus spike protein, wherein the receptor binding domain includes at least 4 non-native. N-linked, glycosylation sites.

公开(公告)号：US12209292B2

公开(公告)日：2025-01-28

申请号：US16328548

申请日：2017-08-25

Applicant: THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Mary Lynn Baniecki ,  Hayden Metsky ,  Pardis Sabeti

IPC: C12Q1/68 ,  C12Q1/6893 ,  C12Q1/70 ,  G16B30/00 ,  G16B30/10

Abstract: The present invention relates to a method for generating primers and/or probes for use in analyzing a sample which may comprise a pathogen target sequence comprising providing a set of input genomic sequence to one or more target pathogens, generating a set of target sequences from the set of input genomic sequences, identifying one or more highly conserved target sequences, and generating one or more primers, one or more probes, or a primer pair and probe combination based on the one or more conserved target sequences.