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公开(公告)号:US20240084363A1
公开(公告)日:2024-03-14
申请号:US18482273
申请日:2023-10-06
申请人: QIAGEN GmbH
IPC分类号: C12Q1/6827 , C12Q1/6806 , C12Q1/6851 , C12Q1/686 , C12Q1/6876 , C12Q1/6879
CPC分类号: C12Q1/6827 , C12Q1/6806 , C12Q1/6851 , C12Q1/686 , C12Q1/6876 , C12Q1/6879 , C12Q2525/151 , C12Q2600/16 , C12Q2600/166
摘要: Oligonucleotide primers and probes for quantifying and/or detecting human male DNA in a forensic sample. The oligonucleotides are useful for amplifying a multicopy locus within the human Y-chromosome (MCL-Y) that shares at least 80% sequence identity to a sequence according to SEQ ID NO. 3 over a stretch of at least 60 base pairs. The oligonucleotides hybridize under stringent conditions to a nucleic acid having at least one sequence selected from the group consisting of SEQ ID NO. 3 to SEQ ID NO. 11 and/or SEQ ID NO. 17 to SEQ ID NO. 25. Kits including the oligonucleotide primers, probes and/or primer pairs and reagents for performing an amplification reaction on DNA recovered from a forensic sample are also disclosed.
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公开(公告)号:US20240124925A1
公开(公告)日:2024-04-18
申请号:US18467758
申请日:2023-09-15
申请人: QIAGEN GmbH
IPC分类号: C12Q1/6851
CPC分类号: C12Q1/6851 , C12Q1/6806
摘要: A method for assessing the status of degradation and/or integrity of one or more nucleic acids in a sample, which includes amplifying at least two overlapping regions within at least one locus and detecting the amount of the amplification products using at least two probes, where one probe binds to an overlapping region and the other probe binds to a non-overlapping region. Also, a method of designing primers and/or probes for amplifying at least two overlapping regions within at least one single copy locus or multicopy locus. Also a primer and a primer pair for amplifying at least two overlapping regions from one nucleic acid template which is a multicopy locus present in loci in the human genome. Also a kit including primers and/or probes for assessing the status of degradation and/or integrity of one or more nucleic acids in a sample.
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公开(公告)号:US20220333176A1
公开(公告)日:2022-10-20
申请号:US17748928
申请日:2022-05-19
申请人: QIAGEN GmbH
IPC分类号: C12Q1/6844 , C12Q1/6848 , C12Q1/6851
摘要: An oligonucleotide suitable for use as a detection probe in a method for evaluating the amplification efficiency, the presence of inhibitors, degradation and/or for performing a quantification analysis of a target nucleic acid in a real-time amplification reaction. The oligonucleotide probe is preferably SEQ ID NO. 4, a reverse complement of SEQ ID NO. 4, an oligonucleotide that shares 95% sequence identity with SEQ ID NO. 4, or an oligonucleotide that shares 95% sequence identity with a reverse complement of SEQ ID NO. 4. Also a kit including an internal nucleic acid control template, a set of primers for amplifying the internal nucleic acid control template, and the oligonucleotide probe.
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公开(公告)号:US20200087720A1
公开(公告)日:2020-03-19
申请号:US16333143
申请日:2017-09-15
申请人: QIAGEN GmbH
IPC分类号: C12Q1/6844 , C12Q1/6848 , C12Q1/6851
摘要: PCR that allows the researchers to amplify a desired DNA requiring only tiny amounts of sample. Such amplification reactions are technically challenging and are often hampered by several practical issues such as the presence of PCR inhibitors, sample degradation and low quantities of said sample. The invention addresses these issues with a method for evaluating the amplification efficiency and/or the presence of inhibitors and/or degradation and/or performing a quantification of a nucleic acid in a real-time amplification reaction comprising: optionally amplifying in a reaction composition a first target nucleic acid using a first primer pair in a real-time amplification reaction, (i) amplifying in said reaction composition one or more second internal nucleic acid control templates (IC) with a length of between 50 and 2000 nucleotides, wherein the second nucleic acid has a sequence selected from the group of: i) SEQ ID NO. 1 or a sequence that is differs by no more than 15% therefrom, ii) a reverse complement of SEQ ID NO. 1 or a sequence that is differs by no more than 15% therefrom, iii) a sequence or a reverse complement thereof comprising the final 18 to 30 3′-nucleotides of SEQ ID NO. 1 at its terminal 3′-end and the final 18 to 30 5′-nucleotides of SEQ ID NO. 1 at its terminal 5′-end, or terminal ends that differ by no more than 5% from SEQ ID NO. 1, wherein between these terminal 3′-ends and 5′-ends the nucleic may have any nucleotide sequence and is between about 30 and about 1950 nucleotides in length.
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公开(公告)号:US20230357823A1
公开(公告)日:2023-11-09
申请号:US18029408
申请日:2021-10-06
申请人: Qiagen GmbH
发明人: Miroslav VRANES , Norbert HOCHSTEIN , Ralf PEIST , Stefan CORNELIUS , Thorsten SINGER , Kerstin STEINERT , Kai TE KAAT
IPC分类号: C12Q1/6806
CPC分类号: C12Q1/6806
摘要: The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.
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6.
公开(公告)号:US20210395801A1
公开(公告)日:2021-12-23
申请号:US17388769
申请日:2021-07-29
申请人: QIAGEN GmbH
IPC分类号: C12Q1/6827 , C12Q1/6879 , C12Q1/6851 , C12Q1/6806 , C12Q1/686 , C12Q1/6876
摘要: Oligonucleotide primers and probes for quantifying and/or detecting human male DNA in a forensic sample. The oligonucleotides are useful for amplifying a multicopy locus within the human Y-chromosome (MCL-Y) that shares at least 80% sequence identity to a sequence according to SEQ ID NO. 3 over a stretch of at least 60 base pairs. The oligonucleotides hybridize under stringent conditions to a nucleic acid having at least one sequence selected from the group consisting of SEQ ID NO. 3 to SEQ ID NO. 11 and/or SEQ ID NO. 17 to SEQ ID NO. 25. Kits including the oligonucleotide primers, probes and/or primer pairs and reagents for performing an amplification reaction on DNA recovered from a forensic sample are also disclosed.
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公开(公告)号:US20190264256A1
公开(公告)日:2019-08-29
申请号:US16333043
申请日:2017-09-15
申请人: QIAGEN GmbH
IPC分类号: C12Q1/6806 , C12Q1/6851
摘要: The present invention relates to a method for assessing the status of nucleic acid degradation and/or integrity of one or more nucleic acids in a sample, comprising the steps amplifying at least two overlapping regions within at least one locus (e.g. by heminested or nested PCR), and detecting the amount of the at least two amplification products through the use of at least two probes, wherein one probe binds to the region of overlap and the at least one other probe binds to a non-overlapping region. The invention further relates to a method of designing primers and/or probes for amplifying at least two overlapping regions within at least one locus, wherein the locus that is amplified is a single copy locus (SCL) or multicopy locus (MLC). The invention also relates to a primer and a primer pair for amplifying at least two overlapping regions from one nucleic acid template which is a multicopy locus present in 21 loci in the human genome. These primers and probes may be in a kit. Hence, the invention also relates to a kit for assessing the status of nucleic acid degradation and/or integrity of one or more nucleic acids in a sample.
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公开(公告)号:US20230374575A1
公开(公告)日:2023-11-23
申请号:US18029937
申请日:2021-10-06
申请人: Qiagen GmbH
发明人: Thorsten SINGER , Miroslav VRANES , Stefan CORNELIUS , Ralf PEIST
IPC分类号: C12Q1/686
CPC分类号: C12Q1/686
摘要: The present invention provides methods and kits for virus inactivation by virus-deactivating substances and direct amplification-based detection of target nucleic acids and viruses in biological samples without prior target purification.
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9.
公开(公告)号:US20230357824A1
公开(公告)日:2023-11-09
申请号:US18029538
申请日:2021-10-06
申请人: Qiagen GmbH
发明人: Miroslav VRANES , Norbert HOCHSTEIN , Ralf PEIST , Stefan CORNELIUS , Thorsten SINGER , Kerstin STEINERT , Kai TE KAAT
IPC分类号: C12Q1/70 , C12Q1/6806
CPC分类号: C12Q1/6806 , C12Q1/701
摘要: The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.
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公开(公告)号:US20220025451A1
公开(公告)日:2022-01-27
申请号:US17500204
申请日:2021-10-13
申请人: QIAGEN GmbH
IPC分类号: C12Q1/6851
摘要: A kit for use in assessing the status of nucleic acid degradation and/or the integrity of one or more nucleic acids in a sample by amplifying at least two overlapping regions within at least one locus and detecting the amount of the at least two amplification products. The kit includes a primer and at least two probes that bind under stringent conditions to a sequence that shares at least 80% sequence identity to a sequence selected from the group of sequences consisting of SEQ ID NO. 6 to SEQ ID NO. 47 over a stretch of 80 base pairs, or to a reverse complement thereof. One of the at least two probes binds to one of the at least two overlapping regions and the other of the at least two probes binds to a non-overlapping region.
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