-
公开(公告)号：US06376170B1
公开(公告)日：2002-04-23
申请号：US08972564
申请日：1997-11-18
IPC分类号： C12Q170
CPC分类号： C07K16/00 , C07K16/087 , C07K2317/21 , C07K2317/55
摘要： The present invention provides a novel method for the identification and clonal isolation of antibodies that bind to unique epitopes. The method is based on the use of antibodies as solid phase capture reagents to bind a known capture antibody epitope, thereby precluding the capture antibody epitope from being presented to a population of antibodies to be screened. The method is particularly suited for screening libraries of cloned antibodies, such as phage display combinatorial antibodies. An antibody specific for herpes simplex virus (HSV), was employed as a model for the assay.
-
公开(公告)号：US07052675B2
公开(公告)日：2006-05-30
申请号：US11027139
申请日：2004-12-29
IPC分类号： A61K39/395 , G01N33/53 , G01N33/567 , C07K16/00
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
摘要翻译： 公开了原位特异性结合天然PrP Sc的抗体。 优选的抗体仅结合特定种类例如人，牛，绵羊，猪等的天然PrP Sc。特别优选的抗体特异性结合人PrP < SUP>。 本发明优选的抗体是（1）通过噬菌体展示法产生，（2）特异性结合天然PrP ，（3）中和朊病毒感染性，（4）结合PrP < Sc（5）在液体可流动的样品中结合50％以上的PrP Sc 。 本发明的抗体可以与底物结合并用于测定样品（其通过蛋白酶K变性的任何PrP c），以获得特异性的PrP PrP
Sc 与疾病相关的物种。 可以标记并注射与人PrP标准物质特异性结合的抗体进行体内诊断测试，以确定人是否感染与疾病相关的朊病毒。 优选使用噬菌体展示技术产生抗体，其中表达抗体的噬菌体中的遗传物质是从具有消融的内源性PrP蛋白基因的哺乳动物和内源性嵌合PrP基因获得的，哺乳动物已用PrP < SUP>以诱导抗体产生。 -
公开(公告)号：US06858397B2
公开(公告)日：2005-02-22
申请号：US10435602
申请日：2003-05-09
IPC分类号： G01N33/53 , C07K16/18 , C07K16/44 , C12N5/10 , C12N15/02 , C12N15/09 , C12P21/08 , G01N33/569 , G01N33/68 , A61K39/395 , C07K16/00 , G01N33/567
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
摘要翻译： 公开了与原位PrP
特异性结合的抗体。 优选的抗体仅与特定物种例如人，牛，绵羊，猪等的天然PrP 。 可以对与人PrP 特异性结合的抗体进行标记和注射进行体内诊断测试，以确定人是否感染与疾病相关的朊病毒。 优选使用噬菌体展示技术产生抗体，其中表达抗体的噬菌体中的遗传物质是从具有消融的内源性PrP蛋白基因的哺乳动物和内源性嵌合PrP基因获得的，哺乳动物用PrP 接种以诱导抗体 生产。 -
公开(公告)号：US06562341B2
公开(公告)日：2003-05-13
申请号：US09943906
申请日：2001-08-30
IPC分类号： A61K39395
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
-
公开(公告)号：US07094553B2
公开(公告)日：2006-08-22
申请号：US10355780
申请日：2003-01-30
IPC分类号： G01N33/53
CPC分类号： C07K16/2872 , C07K16/18 , C07K2317/34 , C07K2317/55 , G01N33/6896 , G01N2800/2828
摘要： The present invention provides antibodies that specifically bind with a high degree of binding affinity to a native ungulate PrPC and/or a denatured ungulate PrPSc, but not to a native ungulate PrPSc. Preferred antibodies find native bovine PrPC and treated PrPSc but not native bovine PrPSc and can be used in an assay to determine if a sample is infected with infectious prions, i.e. PrPSc.
摘要翻译： 本发明提供了与天然有蹄类动物PrP和/或变性有蹄类动物PrP 特异性结合的抗体，而不是天然有蹄类动物 PrP Sc 。 优选的抗体发现天然牛PrP和治疗的PrP 而不是天然牛PrP Sc Sc，并且可用于测定中以确定样品 感染了朊病毒，即PrP Sc 。
-
公开(公告)号：US06537548B1
公开(公告)日：2003-03-25
申请号：US09627218
申请日：2000-07-27
IPC分类号： A61K39395
CPC分类号： C07K16/2872 , C07K16/18 , C07K2317/34 , C07K2317/55 , G01N33/6896 , G01N2800/2828
摘要： The present invention provides antibodies that specifically bind with a high degree of binding affinity to a native ungulate PrPC and/or a denatured ungulate PrPSc, but not to a native ungulate PrPSc. Preferred antibodies find native bovine PrPC and treated PrPSc but not native bovine PrPSc and can be used in an assay to determine if a sample is infected with infectious prions, i.e. PrPSc.
摘要翻译： 本发明提供了与天然有蹄类动物PrPC和/或变性的有蹄类动物PrPSc具有高度结合亲和力的特异性结合的抗体，而不是天然的有蹄类PrPSc结合的抗体。 优选的抗体发现天然牛PrPC和处理的PrPSc而不是天然牛PrPSc，并且可用于测定以确定样品是否感染有感染性朊病毒，即PrPSc。
-
公开(公告)号：US06372214B1
公开(公告)日：2002-04-16
申请号：US09550374
申请日：2000-04-13
IPC分类号： A61K39395
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPSc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
摘要翻译： 公开了与原位PrPSc特异性结合的抗体。 优选的抗体仅结合特定物种例如人，牛，绵羊，猪等的天然PrPSc。特别优选的抗体特异性结合人PrPSc的特异性同种型。 本发明优选的抗体是（1）通过噬菌体展示法产生，（2）特异性结合天然PrPSc，（3）中和朊病毒感染性，（4）原位结合PrPSc，（5）结合50％以上 的PrPSc在液体可流动的样品中。 本发明的抗体可以与底物结合并用于测定PrPSc与疾病相关的特定物种的PrPSc的存在的样品（其通过蛋白酶K变性的任何PrPSc）。 可以对与PrPSc特异性结合的抗体进行标记和注射进行体内诊断测试，以确定人是否感染了与疾病相关的朊病毒。 优选使用噬菌体展示技术制备抗体，其中表达抗体的噬菌体中的遗传物质是从具有消融的内源性PrP蛋白基因的哺乳动物和内源性嵌合PrP基因获得的，哺乳动物已用PrPSc接种以诱导抗体产生。
-
公开(公告)号：US06290954B1
公开(公告)日：2001-09-18
申请号：US09036579
申请日：1998-03-06
IPC分类号： A61K39395
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
摘要翻译： 公开了与原位PrPSc特异性结合的抗体。 优选的抗体仅结合特定物种例如人，牛，绵羊，猪等的天然PrPSc。特别优选的抗体特异性结合人PrPSc的特异性同种型。 本发明优选的抗体是（1）通过噬菌体展示法产生，（2）特异性结合天然PrPSc，（3）中和朊病毒感染性，（4）原位结合PrPSc，（5）结合50％以上 的PrPSc在液体可流动的样品中。 本发明的抗体可以结合于底物并用于测定PrPSc与PrPSc与疾病相关的特定物种的PrPSc的存在的样品（其具有通过蛋白酶K变性的任何PrPc）。 可以对与PrPSc特异性结合的抗体进行标记和注射进行体内诊断测试，以确定人是否感染了与疾病相关的朊病毒。 优选使用噬菌体展示技术制备抗体，其中表达抗体的噬菌体中的遗传物质是从具有消融的内源性PrP蛋白基因的哺乳动物和内源性嵌合PrP基因获得的，哺乳动物已用PrPSc接种以诱导抗体产生。
-
公开(公告)号：US5846533A
公开(公告)日：1998-12-08
申请号：US713939
申请日：1996-09-13
IPC分类号： G01N33/53 , C07K16/18 , C07K16/44 , C12N5/10 , C12N15/02 , C12N15/09 , C12P21/08 , G01N33/569 , G01N33/68 , A61K39/395 , A61K49/00 , C07K16/00
CPC分类号： C07K16/18 , G01N33/56983 , G01N33/6896 , C07K2317/55 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrP.sup.Sc in situ. Preferred antibodies bind only to the native PrP.sup.Sc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrP.sup.Sc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrP.sup.Sc, (3) neutralizes the infectivity of prions, (4) bind to PrP.sup.Sc in situ and (5) bind 50% or more of PrP.sup.Sc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrP.sup.c denatured via proteinase K) for the presence of PrP.sup.Sc of a specific species which PrP.sup.Sc is associated with disease. Antibodies which specifically bind to human PrP.sup.Sc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrP.sup.Sc to induce antibody production.
-
公开(公告)号：US20110135645A1
公开(公告)日：2011-06-09
申请号：US12311587
申请日：2007-10-04
申请人： R. Anthony Williamson , Zhifeng Chen , Pietro Paolo Sanna , Dennis R. Burton , James Crowe , John V. Williams
发明人： R. Anthony Williamson , Zhifeng Chen , Pietro Paolo Sanna , Dennis R. Burton , James Crowe , John V. Williams
CPC分类号： C07K16/1027 , A61K2039/505 , C07K2317/21 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92
摘要： The present invention discloses methods for generating antibodies to human metapneumovirus (HMPV) polypeptides, including antibodies that immunospecifically bind to a HMPV F-protein. The invention also discloses methods for preventing, treating, or ameliorating symptoms associated with HMPV infection.
摘要翻译： 本发明公开了产生针对人类偏肺病毒（HMPV）多肽的抗体的方法，包括免疫特异性结合HMPV F蛋白的抗体。 本发明还公开了用于预防，治疗或改善与HMPV感染相关的症状的方法。
-
-
-
-
-
-
-
-
-
搜索结果
13 条记录 (耗时 0.028 秒)
