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    Cells expressing Pichia cytochrome C
    1.
    发明授权
    Cells expressing Pichia cytochrome C 有权
    表达毕赤酵母细胞色素C的细胞

    公开(公告)号：US07892792B2

    公开(公告)日：2011-02-22

    申请号：US12491909

    申请日：2009-06-25

    申请人： Pundi N Rangarajan ,  Ram Rajasekharan ,  Abhishek Mohanty

    发明人： Pundi N Rangarajan ,  Ram Rajasekharan ,  Abhishek Mohanty

    IPC分类号： C12P21/06 ,  C12P7/64 ,  C12N1/00

    CPC分类号： C07K14/80 ,  C12P7/6427 ,  C12P7/6472 ,  C12P21/02

    摘要： Described herein are recombinant yeast cells that express a cytochrome C gene the expression of which causes the recombinant yeast cells to (i) grow faster than wild type yeast cells of the same species when cultured on glucose medium, and (ii) accumulate high levels of polyunsaturated fatty acid precursor molecules or target gene expression products when grown on oleic acid.

    摘要翻译： 本文描述的是表达细胞色素C基因的重组酵母细胞，其表达导致重组酵母细胞（i）在葡萄糖培养基上培养时，相对于野生型酵母细胞生长得快，并且（ii）积累高水平的 多不饱和脂肪酸前体分子或当在油酸上生长时的靶基因表达产物。

    Triacylglycerol biosynthesis in the cytosol of eukaryotes
    4.
    发明授权
    Triacylglycerol biosynthesis in the cytosol of eukaryotes 失效
    真核生物细胞质中的三酰甘油生物合成

    公开(公告)号：US07229815B2

    公开(公告)日：2007-06-12

    申请号：US10230331

    申请日：2002-08-29

    申请人： Ram Rajasekharan

    发明人： Ram Rajasekharan

    IPC分类号： C12N9/14

    CPC分类号： C12P7/6454 ,  C12N15/52 ,  C12P7/6463

    摘要： This invention describes novel catalytically active cytosolic enzymes for triacylglycerol biosynthesis from eukaryotic systems. The complex from oleaginous yeast was enzymatically characterized, and was found to contain lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, acyl—acyl carrier protein synthetase, superoxide dismutase and acyl carrier protein. The triacylglycerol biosynthetic machinery rapidly incorporates free fatty acids as well as fatty acyl-coenzyme A into triacylglycerol and its biosynthetic intermediates. Lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase and diacylglycerol acyltransferase from the complex were microsequenced. Acyl carrier protein, superoxide dismutase and diacylglycerol acyltransferase genes were cloned and expressed in bacterial system. The soluble triacylglycerol biosynthetic enzymes (lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase) in yeast, rat adipocytes and human hepatocyte cell-line (HepG2) exist in the cytosol either as free enzymes or as a multienzyme complex.

    摘要翻译： 本发明描述了用于真核系统的三酰基甘油生物合成的新型催化活性细胞溶质酶。 含有酵母的复合物经酶学表征，发现含有溶血磷脂酸酰基转移酶，磷脂酸磷酸酶，二酰基甘油酰基转移酶，酰基酰基载体蛋白合成酶，超氧化物歧化酶和酰基载体蛋白。 三酰基甘油生物合成机械快速将游离脂肪酸以及脂肪酰基辅酶A纳入三酰基甘油及其生物合成中间体。 来自复合物的溶血磷脂酰基转移酶，磷脂酸磷酸酶和二酰基甘油酰基转移酶进行微量测序。 酰基载体蛋白，超氧化物歧化酶和二酰基甘油酰基转移酶基因在细菌系统中克隆并表达。 在酵母，大鼠脂肪细胞和人肝细胞细胞系（HepG2）中的可溶性三酰基甘油生物合成酶（溶血磷脂酸酰基转移酶，磷脂酸酸性磷酸酶，二酰基甘油酰基转移酶）作为游离酶或多酶复合物存在于胞质溶胶中。

    Process for preparing a novel synergistic solid/semi-solid organic composition
    7.
    发明授权
    Process for preparing a novel synergistic solid/semi-solid organic composition 失效
    制备新型协同固体/半固体有机组合物的方法

    公开(公告)号：US06391928B1

    公开(公告)日：2002-05-21

    申请号：US09570539

    申请日：2000-05-12

    申请人： Ram Rajasekharan ,  Jaiyanth Daniel

    发明人： Ram Rajasekharan ,  Jaiyanth Daniel

    IPC分类号： A23L105

    CPC分类号： C11B15/00 ,  A23L27/70 ,  B01J13/0065 ,  Y10S514/944

    摘要： This invention relates to a novel synergistic reversible solid/semi-solid organic composition, said composition comprising (a) at least one saturated long chain fatty acid and/or its glycerol esters and (b) one or more liquid neutral organic compounds, said ingredients (a) and (b) being present in a ratio between 0.1 to 40% by weight, and a process for producing said synergistic reversible solid/semi-solid organic composition by mixing the ingredients (a) and (b) defmed above in a ratio between 0.1 to 40% by weight, at a temperature between 2 to 50° C. and at a pressure in the range of 200 torr to 2500 torr.

    摘要翻译： 本发明涉及一种新的协同可逆固体/半固体有机组合物，所述组合物包含（a）至少一种饱和长链脂肪酸和/或其甘油酯和（b）一种或多种液体中性有机化合物，所述成分 （a）和（b）以0.1-40重量％的比例存在，以及通过将上述定义的成分（a）和（b）混合在一起制备所述协同可逆固体/半固体有机组合物的方法 的比例为0.1〜40重量％，温度为2〜50℃，压力为200乇〜2500乇。

    Herbicide comprising phytotoxins of Lasiodiplodia theobromae (LT) fungus, a process of producing the herbicide and a method of using the same
    8.
    发明授权
    Herbicide comprising phytotoxins of Lasiodiplodia theobromae (LT) fungus, a process of producing the herbicide and a method of using the same 失效
    除草剂包括野生型假单胞菌（LT）真菌的植物毒素，除草剂的生产方法及其使用方法

    公开(公告)号：US06277786B1

    公开(公告)日：2001-08-21

    申请号：US09515479

    申请日：2000-02-29

    申请人： Ram Rajasekharan ,  Rosaline Rodrigues ,  Sairam Reddy

    发明人： Ram Rajasekharan ,  Rosaline Rodrigues ,  Sairam Reddy

    IPC分类号： A01N6304

    CPC分类号： A01N63/04

    摘要： The invention relates to describe host-specific extracellular phytotoxins produced by Lasiodiplodia theobromae (LT, also Botryodiplodia theobromae) that has a broad range as a pre-emergent and/or post-emergent bioherbicide, this isolate has been deposited in Microbial Type Culture Collection, Chandigarh, India and given an accession number MTCC 3068, a method using LT-toxin has been developed for controlling certain herbs including Parthenium hysterophorus, duckweeds, jimsonweed, black nightshade, prickly sida and Euphorbia hirta, these phytotoxins can be used partially pure, as a cell-and spore-free filtrate, a crude filtrate, or a crude suspension of the culture and optionally along with other additives.

    摘要翻译： 本发明涉及描述由具有广泛范围的作为出苗前和/或芽后生物除草剂的广泛的无知杆菌（LT，也称为Botryodiplodia theobromae）产生的宿主特异性细胞外植物毒素，该分离物已经保藏在微生物型培养物保藏中， 印度昌迪加，给予加拿大号MTCC 3068，已经开发了一种使用LT-毒素的方法，用于控制某些草药，包括枸杞子，浮萍，吉姆草，黑茄子，刺槐和大戟草，这些植物毒素可以部分纯化，如 细胞和无孢子滤液，粗滤液或培养物的粗悬浮液，以及任选与其它添加剂一起。