公开(公告)号：US08029776B2

公开(公告)日：2011-10-04

申请号：US11866558

申请日：2007-10-03

申请人： Joel R. Haynes ,  Ramani S. Wonderling ,  Dan T. Stinchcomb

发明人： Joel R. Haynes ,  Ramani S. Wonderling ,  Dan T. Stinchcomb

IPC分类号： A61K39/21 ,  C07H23/00 ,  A61K48/00

CPC分类号： A61K39/205 ,  A61K39/0005 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/6018 ,  C12N2760/20134

摘要： The present invention relates to a method to introduce a nucleic acid molecule into a felid by administration of a nucleic acid-cationic lipid complex composition. The method includes the step of administering to the felid, by a parenteral route, a nucleic acid-cationic lipid complex to elicit and/or enhance an immune response. In one embodiment, this method enhances the immune response in a felid compared to a method in which a naked DNA vaccine is administered to a felid. Also provided is a method to deliver a nucleic acid to a felid. This method comprises parenterally administering to the felid a composition that includes a nucleic acid molecule complexed with a cationic lipid.

摘要翻译： 本发明涉及通过施用核酸 - 阳离子脂质复合物组合物将核酸分子引入纤毛蛋白的方法。 该方法包括通过肠胃外途径向核蛋白施用核酸 - 阳离子脂质复合物以引发和/或增强免疫应答的步骤。 在一个实施方案中，与将裸DNA疫苗施用于felid的方法相比，该方法增强了felid中的免疫应答。 还提供了将核酸递送至felid的方法。 该方法包括肠胃外施用包含与阳离子脂质复合的核酸分子的组合物。

公开(公告)号：US07629441B2

公开(公告)日：2009-12-08

申请号：US11369203

申请日：2006-03-06

申请人： Ramani S. Wonderling

发明人： Ramani S. Wonderling

IPC分类号： C07K14/52 ,  C07K14/00 ,  A61K38/21

CPC分类号： C07K14/475 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/51 ,  C07K14/52 ,  C07K14/535 ,  C07K14/54 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5437 ,  C07K14/56 ,  C07K14/70575 ,  C07K14/70578 ,  C07K16/247 ,  C12N2799/021

摘要： The present invention relates to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins; to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF nucleic acid molecules, including those that encode canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins, respectively; to antibodies raised against such proteins; and to inhibitory compounds that regulate such proteins. The present invention also includes methods to identify and obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to regulate an immune response in an animal.

摘要翻译： 本发明涉及犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白细胞介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 对犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF核酸分子，包括 分别编码犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 针对这种蛋白质产生的抗体; 以及调节这些蛋白质的抑制性化合物。 本发明还包括鉴定和获得这些蛋白质，核酸分子，抗体和抑制性化合物的方法。 本发明还包括包含这些蛋白质，核酸分子，抗体和/或抑制化合物的治疗组合物，以及这些治疗组合物在动物体内调节免疫应答的用途。

公开(公告)号：US07932369B2

公开(公告)日：2011-04-26

申请号：US12499625

申请日：2009-07-08

申请人： Ramani S. Wonderling ,  John F. Uher

发明人： Ramani S. Wonderling ,  John F. Uher

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/63 ,  C12N15/00 ,  C12P21/04

CPC分类号： C07K14/47 ,  G01N33/82 ,  Y10T436/11

摘要： An isolated nucleotide sequence or fragment thereof encoding the porcine intrinsic factor, wherein the porcine intrinsic factor comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 6, and SEQ ID NO: 9. The invention also encompasses an isolated nucleic acid sequence or fragment thereof comprising, or complementary to, a nucleotide sequence having at least 85% nucleotide sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, and SEQ ID NO: 7. The porcine intrinsic factor can be use is an assay to determine the quantity of vitamin B12 in a biological sample.

摘要翻译： 编码猪内在因子的分离的核苷酸序列或其片段，其中所述猪内在因子包含与选自SEQ ID NO：3，SEQ ID NO：3的氨基酸序列具有至少85％的氨基酸序列同一性的氨基酸序列 本发明还包括与核苷酸序列具有至少85％核苷酸序列同一性的核苷酸序列或其互补序列的分离的核酸序列或其片段，所述核苷酸序列与选自以下的核苷酸序列具有至少85％的核苷酸序列同一性： SEQ ID NO：1，SEQ ID NO：4和SEQ ID NO：7。猪内在因子可用于测定生物样品中维生素B12的量。

公开(公告)号：US20100285529A1

公开(公告)日：2010-11-11

申请号：US12819964

申请日：2010-06-21

申请人： Ramani S. Wonderling

发明人： Ramani S. Wonderling

IPC分类号： C12P21/02 ,  C07K14/535

CPC分类号： C07K14/475 ,  A61K38/00 ,  A61K2039/51 ,  C07K14/535 ,  C07K14/54 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5437 ,  C07K14/56 ,  C07K14/70575 ,  C07K14/70578 ,  C07K16/247 ,  C12N2799/021

摘要： The present invention relates to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins; to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD 154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF nucleic acid molecules, including those that encode canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD 154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins, respectively; to antibodies raised against such proteins; and to inhibitory compounds that regulate such proteins. The present invention also includes methods to identify and obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to regulate an immune response in an animal.

摘要翻译： 本发明涉及犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白细胞介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 对犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫CD154，犬白细胞介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF核酸分子， 包括编码犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ， 分别; 针对这种蛋白质产生的抗体; 以及调节这些蛋白质的抑制性化合物。 本发明还包括鉴定和获得这些蛋白质，核酸分子，抗体和抑制性化合物的方法。 本发明还包括包含这些蛋白质，核酸分子，抗体和/或抑制化合物的治疗组合物，以及这些治疗组合物在动物体内调节免疫应答的用途。

公开(公告)号：US20090305348A1

公开(公告)日：2009-12-10

申请号：US12499625

申请日：2009-07-08

申请人： Ramani S. Wonderling ,  John F. Uher

发明人： Ramani S. Wonderling ,  John F. Uher

IPC分类号： C12P21/02 ,  C07H21/04 ,  C12N15/63

CPC分类号： C07K14/47 ,  G01N33/82 ,  Y10T436/11

摘要： An isolated nucleotide sequence or fragment thereof encoding the porcine intrinsic factor, wherein the porcine intrinsic factor comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 6, and SEQ ID NO: 9. The invention also encompasses an isolated nucleic acid sequence or fragment thereof comprising, or complementary to, a nucleotide sequence having at least 85% nucleotide sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, and SEQ ID NO: 7. The porcine intrinsic factor can be use is an assay to determine the quantity of vitamin B12 in a biological sample.

摘要翻译： 编码猪内在因子的分离的核苷酸序列或其片段，其中所述猪内在因子包含与选自SEQ ID NO：3，SEQ ID NO：3的氨基酸序列具有至少85％的氨基酸序列同一性的氨基酸序列 本发明还包括与核苷酸序列具有至少85％核苷酸序列同一性的核苷酸序列或其互补序列的分离的核酸序列或其片段，所述核苷酸序列与选自以下的核苷酸序列具有至少85％的核苷酸序列同一性： SEQ ID NO：1，SEQ ID NO：4和SEQ ID NO：7。猪内在因子可用于测定生物样品中维生素B12的量。

公开(公告)号：US06770282B1

公开(公告)日：2004-08-03

申请号：US09830221

申请日：2001-08-10

申请人： Joel R. Haynes ,  Ramani S. Wonderling ,  Dan T. Stinchcomb

发明人： Joel R. Haynes ,  Ramani S. Wonderling ,  Dan T. Stinchcomb

IPC分类号： A61K3939

CPC分类号： A61K39/205 ,  A61K39/0005 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/6018 ,  C12N2760/20134

摘要： The present invention relates to a method to introduce a nucleic acid molecule into a felid by administration of a nucleic acid-cationic lipid complex composition. The method includes the step of administering to the felid, by a parenteral route, a nucleic acid-cationic lipid complex to elicit and/or enhance an immune response. In one embodiment, this method enhances the immune response in a felid compared to a method in which a naked DNA vaccine is administered to a felid. Also provided is a method to deliver a nucleic acid to a felid. This method comprises parenterally administering to the felid a composition that includes a nucleic acid molecule complexed with a cationic lipid.

摘要翻译： 本发明涉及通过施用核酸 - 阳离子脂质复合物组合物将核酸分子引入纤毛蛋白的方法。 该方法包括通过肠胃外途径向核蛋白施用核酸 - 阳离子脂质复合物以引发和/或增强免疫应答的步骤。 在一个实施方案中，与将裸DNA疫苗施用于felid的方法相比，该方法增强了felid中的免疫应答。 还提供了将核酸递送至felid的方法。 该方法包括肠胃外施用包含与阳离子脂质复合的核酸分子的组合物。

公开(公告)号：US07780959B2

公开(公告)日：2010-08-24

申请号：US11486995

申请日：2006-07-14

申请人： Ramani S. Wonderling

发明人： Ramani S. Wonderling

IPC分类号： A61K45/00 ,  C12P21/02 ,  C07K14/00 ,  C07H21/04

CPC分类号： C07K14/475 ,  A61K38/00 ,  A61K2039/51 ,  C07K14/535 ,  C07K14/54 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5437 ,  C07K14/56 ,  C07K14/70575 ,  C07K14/70578 ,  C07K16/247 ,  C12N2799/021

摘要： The present invention relates to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins; to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF nucleic acid molecules, including those that encode canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins, respectively; to antibodies raised against such proteins; and to inhibitory compounds that regulate such proteins. The present invention also includes methods to identify and obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to regulate an immune response in an animal.

摘要翻译： 本发明涉及犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白细胞介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 对犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF核酸分子，包括 分别编码犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 针对这种蛋白质产生的抗体; 以及调节这些蛋白质的抑制性化合物。 本发明还包括鉴定和获得这些蛋白质，核酸分子，抗体和抑制性化合物的方法。 本发明还包括包含这些蛋白质，核酸分子，抗体和/或抑制化合物的治疗组合物，以及这些治疗组合物在动物体内调节免疫应答的用途。

公开(公告)号：US20100124754A1

公开(公告)日：2010-05-20

申请号：US12275102

申请日：2008-11-20

申请人： Ramani S. Wonderling ,  John F. Uher ,  Thomas P. Leary ,  You Pan

发明人： Ramani S. Wonderling ,  John F. Uher ,  Thomas P. Leary ,  You Pan

IPC分类号： C12P21/00 ,  C07K14/435 ,  G01N33/53

CPC分类号： C07K14/47 ,  G01N33/82

摘要： The present invention relates to a new method of producing functionally active recombinant porcine Intrinsic Factor as well as the protein (i.e., porcine Intrinsic Factor) produced thereby. In particular, the vector comprising the DNA encoding the protein is introduced into a Chinese Hamster Ovary (CHO) host cell under conditions sufficient for optimal expression of functional protein. The expressed protein may then be subjected to three methods ultimately resulting in a protein having at least 97% purity.

摘要翻译： 本发明涉及生产功能活性的重组猪本征因子以及由此产生的蛋白质（即猪内在因子）的新方法。 特别地，将包含编码该蛋白质的DNA的载体在足以使功能性蛋白质最佳表达的条件下导入中国仓鼠卵巢（CHO）宿主细胞。 然后可以使表达的蛋白质经受三种方法，最终产生具有至少97％纯度的蛋白质。

公开(公告)号：US06482403B1

公开(公告)日：2002-11-19

申请号：US09451527

申请日：1999-12-01

申请人： Gek-Kee Sim ,  Shumin Yang ,  Matthew J. Dreitz ,  Ramani S. Wonderling

发明人： Gek-Kee Sim ,  Shumin Yang ,  Matthew J. Dreitz ,  Ramani S. Wonderling

IPC分类号： A61K4500

CPC分类号： C07K14/475 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/51 ,  C07K14/52 ,  C07K14/535 ,  C07K14/54 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5437 ,  C07K14/56 ,  C07K14/70575 ,  C07K14/70578 ,  C07K16/247 ,  C12N2799/021

摘要： The present invention relates to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins; to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF nucleic acid molecules, including those that encode canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins, respectively; to antibodies raised against such proteins; and to inhibitory compounds that regulate such proteins. The present invention also includes methods to identify and obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to regulate an immune response in an animal.

摘要翻译： 本发明涉及犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白细胞介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 对犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF核酸分子，包括 分别编码犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 针对这种蛋白质产生的抗体; 以及调节这些蛋白质的抑制性化合物。 本发明还包括鉴定和获得这些蛋白质，核酸分子，抗体和抑制性化合物的方法。 本发明还包括包含这些蛋白质，核酸分子，抗体和/或抑制化合物的治疗组合物，以及这些治疗组合物在动物体内调节免疫应答的用途。

公开(公告)号：US06471957B1

公开(公告)日：2002-10-29

申请号：US09322409

申请日：1999-05-28

申请人： Gek-Kee Sim ,  Shumin Yang ,  Matthew J. Dreitz ,  Ramani S. Wonderling

发明人： Gek-Kee Sim ,  Shumin Yang ,  Matthew J. Dreitz ,  Ramani S. Wonderling

IPC分类号： A61K4500

CPC分类号： C07K14/475 ,  A61K38/00 ,  A61K2039/51 ,  C07K14/535 ,  C07K14/54 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5437 ,  C07K14/56 ,  C07K14/70575 ,  C07K14/70578 ,  C07K16/247 ,  C12N2799/021

摘要： The present invention relates to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins; to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF nucleic acid molecules, including those that encode canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins, respectively; to antibodies raised against such proteins; and to inhibitory compounds that regulate such proteins. The present invention also includes methods to identify and obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to regulate an immune response in an animal.

摘要翻译： 本发明涉及犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白细胞介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 对犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF核酸分子，包括 分别编码犬白细胞介素-4，犬或猫Flt-3配体，犬或猫CD40，犬或猫科动物CD154，犬白介素-5，犬白细胞介素-13，猫干扰素α和/或猫GM-CSF蛋白 ; 针对这种蛋白质产生的抗体; 以及调节这些蛋白质的抑制性化合物。 本发明还包括鉴定和获得这些蛋白质，核酸分子，抗体和抑制性化合物的方法。 本发明还包括包含这些蛋白质，核酸分子，抗体和/或抑制化合物的治疗组合物，以及这些治疗组合物在动物体内调节免疫应答的用途。