利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

27 条记录 (耗时 0.022 秒)

    Cloning, Expression And Purification Of Recombinant Porcine Intrinsic Factor For Use In Diagnostic Assay
    2.
    发明申请
    Cloning, Expression And Purification Of Recombinant Porcine Intrinsic Factor For Use In Diagnostic Assay 有权
    克隆，表达和纯化重组猪内在因子用于诊断测定

    公开(公告)号：US20100124754A1

    公开(公告)日：2010-05-20

    申请号：US12275102

    申请日：2008-11-20

    申请人： Ramani S. Wonderling ,  John F. Uher ,  Thomas P. Leary ,  You Pan

    发明人： Ramani S. Wonderling ,  John F. Uher ,  Thomas P. Leary ,  You Pan

    IPC分类号： C12P21/00 ,  C07K14/435 ,  G01N33/53

    CPC分类号： C07K14/47 ,  G01N33/82

    摘要： The present invention relates to a new method of producing functionally active recombinant porcine Intrinsic Factor as well as the protein (i.e., porcine Intrinsic Factor) produced thereby. In particular, the vector comprising the DNA encoding the protein is introduced into a Chinese Hamster Ovary (CHO) host cell under conditions sufficient for optimal expression of functional protein. The expressed protein may then be subjected to three methods ultimately resulting in a protein having at least 97% purity.

    摘要翻译： 本发明涉及生产功能活性的重组猪本征因子以及由此产生的蛋白质（即猪内在因子）的新方法。 特别地，将包含编码该蛋白质的DNA的载体在足以使功能性蛋白质最佳表达的条件下导入中国仓鼠卵巢（CHO）宿主细胞。 然后可以使表达的蛋白质经受三种方法，最终产生具有至少97％纯度的蛋白质。

    INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME
    7.
    发明申请
    INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME 审中-公开
    不同标记的测定结合物及其制备方法和使用方法

    公开(公告)号：US20120329176A1

    公开(公告)日：2012-12-27

    申请号：US13600979

    申请日：2012-08-31

    申请人： Anthony S. Muerhoff ,  Suresh M. Desai ,  Thomas P. Leary ,  George J. Dawson ,  Robin A. Gutierrez

    发明人： Anthony S. Muerhoff ,  Suresh M. Desai ,  Thomas P. Leary ,  George J. Dawson ,  Robin A. Gutierrez

    IPC分类号： C07K1/13 ,  G01N21/76 ,  C07K19/00

    CPC分类号： G01N33/56983 ,  C07K1/13 ,  G01N33/532 ,  G01N2333/186

    摘要： Indirectly labelled assay conjugates prepared by a method that includes the step of submitting the binding member comprised by the conjugate to denaturing conditions prior to labelling the binding member. The indirectly labelled assay conjugates demonstrate an increased sensitivity when employed in diagnostic assays compared to assay conjugates prepared by methods that do not include a step of submitting the binding member to denaturing conditions prior to labelling. Processes for the preparation of the indirectly labelled assay conjugates, methods of detecting an analyte comprising the use of the indirectly labelled assay conjugate and kits comprising the indirectly labelled conjugates are also provided.

    摘要翻译： 间接标记的测定缀合物，其通过包括将缀合物包含的结合成员在标记结合成员之前提交至变性条件的方法制备。 间接标记的测定缀合物在用于诊断测定中的灵敏度增加，与通过不包括在标记之前将结合成员提交至变性条件的步骤的方法制备的测定缀合物相比较。 还提供了用于制备间接标记的测定缀合物的方法，检测包括使用间接标记的测定缀合物的分析物的方法和包含间接标记的缀合物的试剂盒。

    INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME
    8.
    发明申请
    INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME 审中-公开
    不同标记的测定结合物及其制备方法和使用方法

    公开(公告)号：US20100279432A1

    公开(公告)日：2010-11-04

    申请号：US12832262

    申请日：2010-07-08

    申请人： Anthony S. Muerhoff ,  Suresh M. Desai ,  Thomas P. Leary ,  George J. Dawson ,  Robin A. Gutierrez

    发明人： Anthony S. Muerhoff ,  Suresh M. Desai ,  Thomas P. Leary ,  George J. Dawson ,  Robin A. Gutierrez

    IPC分类号： G01N33/53 ,  C07K1/13 ,  C07K14/765 ,  C07K14/435 ,  C07K14/77

    CPC分类号： G01N33/56983 ,  C07K1/13 ,  G01N33/532 ,  G01N2333/186

    摘要： Indirectly labelled assay conjugates prepared by a method that includes the step of submitting the binding member comprised by the conjugate to denaturing conditions prior to labelling the binding member. The indirectly labelled assay conjugates demonstrate an increased sensitivity when employed in diagnostic assays compared to assay conjugates prepared by methods that do not include a step of submitting the binding member to denaturing conditions prior to labelling. Processes for the preparation of the indirectly labelled assay conjugates, methods of detecting an analyte comprising the use of the indirectly labelled assay conjugate and kits comprising the indirectly labelled conjugates are also provided.

    摘要翻译： 间接标记的测定缀合物，其通过包括将缀合物包含的结合成员在标记结合成员之前提交至变性条件的方法制备。 间接标记的测定缀合物在用于诊断测定中的灵敏度增加，与通过不包括在标记之前将结合成员提交至变性条件的步骤的方法制备的测定缀合物相比较。 还提供了用于制备间接标记的测定缀合物的方法，检测包括使用间接标记的测定缀合物的分析物的方法和包含间接标记的缀合物的试剂盒。