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公开(公告)号：US5466591A
公开(公告)日：1995-11-14
申请号：US977434
申请日：1993-02-23
CPC分类号： C12P19/34 , C12N15/70 , C12N9/1252 , C12N9/1276
摘要： The present invention relates to thermostable DNA polymerases which have been mutated such that a lesser amount of 5' to 3' exonuclease activity is exhibited from that which is exhibited by the native enzyme.
摘要翻译： PCT No.PCT / US91 / 07035 Sec。 371日期：1993年2月23日 102（e）日期1993年2月23日PCT 1991年9月30日PCT公布。 出版物WO92 / 06200 本发明涉及已经突变的热稳定性DNA聚合酶，使得从天然酶显示的5'至3'外切核酸酶活性显示较少量。
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2.发明授权DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 失效编码来自热带菌种sps17的突变的热稳定性核酸聚合酶的DNA
公开(公告)号：US5405774A
公开(公告)日：1995-04-11
申请号：US119754
申请日：1993-09-10
CPC分类号： C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
摘要： A purified thermostable enzyme is derived from the bacterium Thermus species sps17. The enzyme has DNA polymerase activity, reverse transcriptase activity, and optionally, 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and can be used with selected primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
摘要翻译： 纯化的热稳定酶源自嗜热菌菌种sps17。 该酶具有DNA聚合酶活性，逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与选定的引物和三磷酸核苷一起使用，其中至少一个核酸序列的量从现有的序列扩增。
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公开(公告)号：US5795762A
公开(公告)日：1998-08-18
申请号：US458819
申请日：1995-06-02
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： The present invention relates to thermostable DNA polymerases which exhibit a different level of 5' to 3' exonuclease activity than their respective native polymerases. Particular conserved amino acid domains in thermostable DNA polymerases are mutated or deleted to alter the 5' to 3' exonuclease activity of the polymerases. The present invention also relates to means for isolating and producing such altered polymerases.
摘要翻译： 本发明涉及耐热性DNA聚合酶，其表现出与其各自的天然聚合酶不同的5'至3'核酸外切酶活性水平。 热稳定DNA聚合酶中特定的保守氨基酸结构域突变或缺失，以改变聚合酶的5'至3'核酸外切酶活性。 本发明还涉及用于分离和产生这种改变的聚合酶的方法。
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4.发明授权DNA encoding thermostable nucleic acid polymerase enzyme from thermus species Z05 失效从热带菌Z05编码热稳定性核酸聚合酶的DNA
公开(公告)号：US5674738A
公开(公告)日：1997-10-07
申请号：US520422
申请日：1995-08-29
CPC分类号： C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
摘要： A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
摘要翻译： 纯化的热稳定酶源自嗜热菌菌种Z05。 该酶具有DNA聚合酶，活性逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与引物和核苷三磷酸一起使用，其中至少一个核酸序列以现有序列的量被扩增。
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5.发明授权Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 失效来自Thermus物种Z05的突变的热稳定性核酸聚合酶
公开(公告)号：US5455170A
公开(公告)日：1995-10-03
申请号：US113531
申请日：1993-08-27
CPC分类号： C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
摘要： A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
摘要翻译： 纯化的热稳定酶源自嗜热菌菌种Z05。 该酶具有DNA聚合酶，活性逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与引物和核苷三磷酸一起使用，其中至少一个核酸序列以现有序列的量被扩增。
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