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公开(公告)号：US5795762A
公开(公告)日：1998-08-18
申请号：US458819
申请日：1995-06-02
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： The present invention relates to thermostable DNA polymerases which exhibit a different level of 5' to 3' exonuclease activity than their respective native polymerases. Particular conserved amino acid domains in thermostable DNA polymerases are mutated or deleted to alter the 5' to 3' exonuclease activity of the polymerases. The present invention also relates to means for isolating and producing such altered polymerases.
摘要翻译： 本发明涉及耐热性DNA聚合酶，其表现出与其各自的天然聚合酶不同的5'至3'核酸外切酶活性水平。 热稳定DNA聚合酶中特定的保守氨基酸结构域突变或缺失，以改变聚合酶的5'至3'核酸外切酶活性。 本发明还涉及用于分离和产生这种改变的聚合酶的方法。
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公开(公告)号：US5466591A
公开(公告)日：1995-11-14
申请号：US977434
申请日：1993-02-23
CPC分类号： C12P19/34 , C12N15/70 , C12N9/1252 , C12N9/1276
摘要： The present invention relates to thermostable DNA polymerases which have been mutated such that a lesser amount of 5' to 3' exonuclease activity is exhibited from that which is exhibited by the native enzyme.
摘要翻译： PCT No.PCT / US91 / 07035 Sec。 371日期：1993年2月23日 102（e）日期1993年2月23日PCT 1991年9月30日PCT公布。 出版物WO92 / 06200 本发明涉及已经突变的热稳定性DNA聚合酶，使得从天然酶显示的5'至3'外切核酸酶活性显示较少量。
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3.发明授权DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 失效编码来自热带菌种sps17的突变的热稳定性核酸聚合酶的DNA
公开(公告)号：US5405774A
公开(公告)日：1995-04-11
申请号：US119754
申请日：1993-09-10
CPC分类号： C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
摘要： A purified thermostable enzyme is derived from the bacterium Thermus species sps17. The enzyme has DNA polymerase activity, reverse transcriptase activity, and optionally, 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and can be used with selected primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
摘要翻译： 纯化的热稳定酶源自嗜热菌菌种sps17。 该酶具有DNA聚合酶活性，逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与选定的引物和三磷酸核苷一起使用，其中至少一个核酸序列的量从现有的序列扩增。
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4.发明授权DNA encoding thermostable nucleic acid polymerase enzyme from thermus species Z05 失效从热带菌Z05编码热稳定性核酸聚合酶的DNA
公开(公告)号：US5674738A
公开(公告)日：1997-10-07
申请号：US520422
申请日：1995-08-29
CPC分类号： C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
摘要： A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
摘要翻译： 纯化的热稳定酶源自嗜热菌菌种Z05。 该酶具有DNA聚合酶，活性逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与引物和核苷三磷酸一起使用，其中至少一个核酸序列以现有序列的量被扩增。
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5.发明授权Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 失效来自Thermus物种Z05的突变的热稳定性核酸聚合酶
公开(公告)号：US5455170A
公开(公告)日：1995-10-03
申请号：US113531
申请日：1993-08-27
CPC分类号： C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
摘要： A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
摘要翻译： 纯化的热稳定酶源自嗜热菌菌种Z05。 该酶具有DNA聚合酶，活性逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与引物和核苷三磷酸一起使用，其中至少一个核酸序列以现有序列的量被扩增。
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公开(公告)号：US20080293071A1
公开(公告)日：2008-11-27
申请号：US12158257
申请日：2006-12-20
申请人： David H. Gelfand , Amar Gupta
发明人： David H. Gelfand , Amar Gupta
CPC分类号： C12Q1/6869 , C12Q2525/186 , C12Q2521/525 , C12Q2521/319 , C12Q2521/301
摘要： The invention provides a method of determining the nucleotide sequence of a target nucleic acid using a reversibly terminating nucleotide that is modified at the 2′ position.
摘要翻译： 本发明提供了使用在2'位置修饰的可逆终止的核苷酸来确定靶核酸的核苷酸序列的方法。
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公开(公告)号：US06514736B1
公开(公告)日：2003-02-04
申请号：US09704357
申请日：2000-11-01
IPC分类号： C12N912
CPC分类号： C12Q1/6858 , B01J19/0046 , B01J2219/0059 , B01J2219/00722 , B01L7/52 , C07K14/70539 , C07K14/805 , C12N9/1252 , C12N15/10 , C12P19/34 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , C40B40/06 , Y10S977/92 , C12Q2549/119 , C12Q2525/149 , C12Q2525/143 , C12Q2525/131
摘要： The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译： 本发明涉及使用热稳定性酶扩增核酸或其混合物中包含的任何靶核酸序列的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的分开的互补链，用热稳定酶扩增引物以形成互补引物延伸产物，其用作合成所需核酸序列的模板，并检测序列 放大 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。
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8.发明授权Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents 失效含有非离子聚合物洗涤剂的稳定的耐热核酸聚合酶组合物
公开(公告)号：US6127155A
公开(公告)日：2000-10-03
申请号：US873897
申请日：1992-04-24
CPC分类号： C12P19/34 , C12N15/70 , C12N9/1252 , C12Q1/686 , Y10S435/814
摘要： A purified thermostable nucleic acid polymerase is obtained that has unique characteristics. Preferably the nucleic acid polymerase is DNA polymerase isolated from a Thermus aquaticus species and has a molecular weight of about 86,000-95,000 daltons. The thermostable nucleic acid polymerase may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The nucleic acid polymerase is preferably stored in a buffer containing non-ionic detergents that lends stability to the nucleic acid polymerase. A preferred buffer contains glycerol, polyoxyethylated sorbitan monolaurate, ethoxylated nonyl phenol and gelatin.
摘要翻译： 获得了具有独特特征的纯化的热稳定性核酸聚合酶。 优选地，所述核酸聚合酶是从栖热栖热菌属物种分离的DNA聚合酶，其分子量约为86,000-95,000道尔顿。 热稳定性核酸聚合酶可以是天然的或重组的，并且可以用于温度循环链式反应，其中借助于选定的引物和三磷酸核苷酸，从现存的序列中扩增至少一个核酸序列。 核酸聚合酶优选储存在含有使核酸聚合酶具有稳定性的非离子型清净剂的缓冲液中。 优选的缓冲液含有甘油，聚氧乙基化脱水山梨糖醇单月桂酸酯，乙氧基化壬基苯酚和明胶。
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9.发明授权
公开(公告)号：US6040166A
公开(公告)日：2000-03-21
申请号：US312729
申请日：1994-09-27
IPC分类号： B01J19/00 , B01L7/00 , C07K14/74 , C07K14/805 , C12N9/12 , C12N15/10 , C12P19/34 , C12Q1/68 , C40B40/06
CPC分类号： C12Q1/6858 , B01J19/0046 , B01L7/52 , C07K14/70539 , C07K14/805 , C12N15/10 , C12N9/1252 , C12P19/34 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , B01J2219/0059 , B01J2219/00722 , C40B40/06 , Y10S977/92
摘要： The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译： 本发明涉及使用热稳定性酶扩增核酸或其混合物中包含的任何靶核酸序列的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的分开的互补链，用热稳定酶扩增引物以形成互补引物延伸产物，其用作合成所需核酸序列的模板，并检测序列 放大 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。
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公开(公告)号：US5759828A
公开(公告)日：1998-06-02
申请号：US309512
申请日：1994-09-20
申请人： Rony Tal , David H. Gelfand , Roger D. Calhoon , Arie Ben-Bassat , Moshe Benziman , Hing Cheung Wong
发明人： Rony Tal , David H. Gelfand , Roger D. Calhoon , Arie Ben-Bassat , Moshe Benziman , Hing Cheung Wong
IPC分类号： C07K14/195 , C07K14/41 , C12N1/21 , C12N9/10 , C12N9/16 , C12N9/88 , C12N15/09 , C12N15/52 , C12P19/04 , C12P21/02 , C12R1/02 , C12N15/63 , C12N15/70 , C12N15/74 , C12P1/04
CPC分类号： C12N9/16 , C12N15/52 , C12N9/1051 , C12N9/88 , C12P19/04
摘要： The present invention provides the nucleotide sequences of Acetobacter operons, cdg operons encoding genes for the biosynthesis and degradation of cyclic diguanosine monophosphate (c-di-GMP). Specifically, the nucleotide sequences and deduced amino acid sequences of 3 phosphodiesterases isozymes, 3 diguanylate cyclase isozymes, and 2 polypeptides of unidentified function are provided. Also provided for are various strains of microorganisms, including Acetobacter cells genetically manipulated so as to produce elevated and/or reduced levels of one or more cdg operon encoded proteins.
摘要翻译： 本发明提供了醋酸杆菌操纵子的核苷酸序列，编码用于生物合成和环状二磷酸腺苷（c-di-GMP）的降解的基因的cdg操纵子。 具体地，提供了3种磷酸二酯酶同工酶，3个二环酸酯环化酶同功酶和2个未鉴定功能的多肽的核苷酸序列和推导的氨基酸序列。 还提供了各种微生物菌株，包括遗传操纵的醋杆菌细胞，以便产生升高和/或降低的一种或多种cdg操纵子编码的蛋白质的水平。
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