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    Compositions and methods for capture and elution of biological materials via particulates
    6.
    发明授权
    Compositions and methods for capture and elution of biological materials via particulates 有权
    通过微粒捕获和洗脱生物材料的组合物和方法

    公开(公告)号：US08663974B2

    公开(公告)日：2014-03-04

    申请号：US13336932

    申请日：2011-12-23

    申请人： Mark Brown ,  Robert Doebler ,  Bruce Irvine ,  Tanya Ferguson ,  Gary Blackburn

    发明人： Mark Brown ,  Robert Doebler ,  Bruce Irvine ,  Tanya Ferguson ,  Gary Blackburn

    IPC分类号： C12M1/00 ,  C12Q1/00 ,  C12Q1/68 ,  C12Q1/02

    CPC分类号： C12N1/06 ,  B01L3/563 ,  B01L3/567 ,  C12M47/06 ,  G01N33/54313 ,  G01N2001/2866 ,  G01N2001/4088

    摘要： Lysing may include agitating a specimen in a chamber along with a medium that includes a particulate lysing material that has an affinity for a biological material. Lysing material may include beads or other material which may be coated that facilitates binding. The medium may include a fluid with a high salt or low pH level. Binding of biological materials to solid surfaces may be induced by particular media. The biological material may be eluted by lowering a concentration of salt or increasing a pH level. Lysing materials with two or more different affinities may be employed. Lysing materials may include particles of different sizes. Heating may be used. Lysing may be performed in a flow through apparatus. Surfaces of solid materials may be modified to capture bacteria with high cell wall lipid content.

    摘要翻译： 裂解可以包括在室中搅拌样品以及包含对生物材料具有亲和力的颗粒裂解材料的培养基。 裂解材料可以包括可以被涂覆的珠或其它材料，其有助于结合。 介质可以包括具有高盐或低pH水平的流体。 生物材料与固体表面的结合可以由特定的介质诱导。 可以通过降低盐的浓度或增加pH水平来洗脱生物材料。 可以采用具有两种或更多种不同亲和力的裂解材料。 裂解材料可以包括不同尺寸的颗粒。 可以使用加热。 裂解可以在流过设备中进行。 可以修饰固体材料的表面以捕获具有高细胞壁脂质含量的细菌。