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    Methods for modulating cell proliferation in the seed coat and/or integument
    1.
    发明授权
    Methods for modulating cell proliferation in the seed coat and/or integument 有权
    调节种皮和/或皮肤中细胞增殖的方法

    公开(公告)号：US09534228B2

    公开(公告)日：2017-01-03

    申请号：US13114656

    申请日：2011-05-24

    Applicant: Roderick Scott

    Inventor： Roderick Scott

    IPC: C12N15/82 ,  A01H5/10 ,  C07K14/415

    CPC classification number: C12N15/8261 ,  A01H5/10 ,  C07K14/415 ,  C12N15/823 ,  C12N15/8234 ,  Y02A40/146

    Abstract: This invention relates to methods for altering cell proliferation in a plant by modulating the expression of an endogenous plant gene whose expression product affects cell proliferation. These methods can be used to modify plant characteristics, such as seed size. In particular, the invention provides methods for modifying cell proliferation in the integuments and/or seed coats of a plant by inhibiting expression of endogenous gene encoding Auxin response factor 2 (ARF2/MNT) or an orthologue thereof, such that the number of cells in the integuments and/or seed coat is increased, resulting in increased seed size. The plant or plant propagating material can be transformed, e.g., with a nucleic acid construct, to increase or decrease the expression of endogenous genes or the orthologues thereof that affect cell proliferation. The invention also relates to modified plants and reproducible plant material produced by the methods, and nucleic acid constructs for use in such methods.

    Abstract translation: 本发明涉及通过调节其表达产物影响细胞增殖的内源植物基因的表达来改变植物细胞增殖的方法。 这些方法可用于修改植物特性，如种子大小。 特别地，本发明提供了通过抑制编码生物素应答因子2（ARF2 / MNT）或其直向同源物的内源基因的表达来修饰植物的外皮和/或种皮中的细胞增殖的方法， 皮和/或种皮增加，导致种子大小增加。 植物或植物繁殖材料可以例如用核酸构建体进行转化，以增加或减少影响细胞增殖的内源基因或其直向同源物的表达。 本发明还涉及通过所述方法生产的经修饰的植物和可重复植物材料，以及用于这些方法的核酸构建体。

    DI-SUBSTITUTED PYRIDINUM POLYMERS AND SYNTHESIS THEREOF
    2.
    发明申请
    DI-SUBSTITUTED PYRIDINUM POLYMERS AND SYNTHESIS THEREOF 审中-公开
    二取代的PYRIDINUM聚合物及其合成

    公开(公告)号：US20120123113A1

    公开(公告)日：2012-05-17

    申请号：US13146566

    申请日：2010-01-27

    Applicant: Marcel Jaspars ,  Wael Houssen ,  Zhibao Lu ,  Roderick Scott ,  RuAngelie Edrada-ebel ,  Ines Mancini

    Inventor： Marcel Jaspars ,  Wael Houssen ,  Zhibao Lu ,  Roderick Scott ,  RuAngelie Edrada-ebel ,  Ines Mancini

    IPC: C07D487/22 ,  C07D401/14

    CPC classification number: C08G73/0627

    Abstract: A method of producing a di-substituted pyridinium polymer by microwave-assisted polymerisation of a 2, 3, or 4-substituted pyridine monomer of the formula NC5R4—R′—X, wherein R is selected from hydrogen, hydroxyl, and substituted or unsubstituted alkyl, alkoxy, aryl, alkaryl, aralkyl, and alkenyl groups, R′ is a linking group, and X is a leaving group. Using this method, di-substituted pyridinium polymer compositions may be obtained wherein at least 50% of the di- substituted pyridinium polymer chains in the composition have the same degree of polymerisation.

    Abstract translation: 通过微波辅助聚合式NC5R4-R'-X的2,3或4-取代吡啶单体制备二取代吡啶鎓聚合物的方法，其中R选自氢，羟基和取代或未取代的 烷基，烷氧基，芳基，烷芳基，芳烷基和烯基，R'是连接基团，X是离去基团。 使用该方法，可以获得二取代的吡啶鎓聚合物组合物，其中组合物中至少50％的二取代的吡啶鎓聚合物链具有相同的聚合度。

    SEEDS
    4.
    发明申请
    SEEDS 审中-公开
    种子

    公开(公告)号：US20120159671A1

    公开(公告)日：2012-06-21

    申请号：US13114656

    申请日：2011-05-24

    Applicant: RODERICK SCOTT

    Inventor： RODERICK SCOTT

    IPC: C12N15/82

    CPC classification number: C12N15/8261 ,  A01H5/10 ,  C07K14/415 ,  C12N15/823 ,  C12N15/8234 ,  Y02A40/146

    Abstract: This invention relates to a method for modifying cell proliferation in a plant which comprises the step of transforming a plant, or plant propagating material, with a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within the integuments and/or seed coat and at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulating cell proliferation, whereby on expression of that nucleic acid sequence cell proliferation is modified. The invention also relates to a plant which includes a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within integuments and/or seed coat an at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulated cell proliferation, whereby on expression of that nucleic acid sequence cell proliferation is modified. The invention also relates to reproducible plant material including a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within integuments and/or seed coat and at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulated cell proliferation, whereby expression of that nucleic acid sequence cell proliferation is modified. According to another aspect of the invention, there is provided a method for modifying cell proliferation in a plant which comprises the step of modulating the response of the plant to an auxin in which cell proliferation is modified to produce larger or smaller seeds than wild-type.

    Abstract translation: 本发明涉及一种用于修饰植物细胞增殖的方法，该方法包括用包含至少一个能够在皮肤和/或种皮中表达的调控序列的核酸分子转化植物或植物繁殖材料的步骤 和至少一个其表达或转录产物能够直接或间接调节细胞增殖的核酸序列，从而在该核酸序列的表达细胞增殖被修饰。 本发明还涉及一种植物，其包含核酸分子，所述核酸分子包含至少一个能够引导表皮内和/或种皮的调节序列，所述调节序列的表达或转录产物能够直接或间接调节细胞的至少一个核酸序列 增殖，由此在该核酸序列的表达细胞增殖被修饰。 本发明还涉及可再现的植物材料，其包括核酸分子，其包含至少一个能够引导表皮和/或种皮中的表达的调节序列和至少一个其表达或转录产物能够直接或间接调节细胞的核酸序列 从而改变该核酸序列细胞增殖的表达。 根据本发明的另一方面，提供了一种修饰植物细胞增殖的方法，其包括调节植物对生长素的反应的步骤，其中细胞增殖被修饰以产生比野生型更大或更小的种子 。

    Seeds
    8.
    发明申请
    Seeds 审中-公开
    种子

    公开(公告)号：US20080052793A1

    公开(公告)日：2008-02-28

    申请号：US10591418

    申请日：2005-03-07

    Applicant: Roderick Scott

    Inventor： Roderick Scott

    IPC: C12N15/82 ,  A01H5/00

    CPC classification number: C12N15/8261 ,  A01H5/10 ,  C07K14/415 ,  C12N15/823 ,  C12N15/8234 ,  Y02A40/146

    Abstract: This invention relates to a method for modifying cell proliferation in a plant which comprises the step of transforming a plant, or plant propagating material, with a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within the integuments and/or seed coat and at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulating cell proliferation, whereby on expression of that nucleic acid sequence cell proliferation is modified. The invention also relates to a plant which includes a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within integuments and/or seed coat an at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulated cell proliferation, whereby on expression of that nucleic acid sequence cell proliferation is modified. The invention also relates to reproducible plant material including a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within integuments and/or seed coat and at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulated cell proliferation, whereby expression of that nucleic acid sequence cell proliferation is modified. According to another aspect of the invention, there is provided a method for modifying cell proliferation in a plant which comprises the step of modulating the response of the plant to an auxin in which cell proliferation is modified to produce larger or smaller seeds than wild-type.

    Abstract translation: 本发明涉及一种用于修饰植物细胞增殖的方法，该方法包括用包含至少一个能够在皮肤和/或种皮中表达的调控序列的核酸分子转化植物或植物繁殖材料的步骤 和至少一个其表达或转录产物能够直接或间接调节细胞增殖的核酸序列，从而在该核酸序列的表达细胞增殖被修饰。 本发明还涉及一种植物，其包含核酸分子，所述核酸分子包含至少一个能够引导表皮内和/或种皮的调节序列，所述调节序列的表达或转录产物能够直接或间接调节细胞的至少一个核酸序列 增殖，由此在该核酸序列的表达细胞增殖被修饰。 本发明还涉及可再现的植物材料，其包括核酸分子，其包含至少一个能够引导表皮和/或种皮中的表达的调节序列和至少一个其表达或转录产物能够直接或间接调节细胞的核酸序列 从而改变该核酸序列细胞增殖的表达。 根据本发明的另一方面，提供了一种修饰植物细胞增殖的方法，其包括调节植物对生长素的反应的步骤，其中细胞增殖被修饰以产生比野生型更大或更小的种子 。

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