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公开(公告)号：US08158404B2
公开(公告)日：2012-04-17
申请号：US13076284
申请日：2011-03-30
申请人： Doug Lies , Stephanie Porter-Scheinman , Julie Kelly , Catherine Asleson Dundon , Aristos Aristidou , Andrew Hawkins
发明人： Doug Lies , Stephanie Porter-Scheinman , Julie Kelly , Catherine Asleson Dundon , Aristos Aristidou , Andrew Hawkins
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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公开(公告)号：US08153415B2
公开(公告)日：2012-04-10
申请号：US13077170
申请日：2011-03-31
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含生物合成途径的重组微生物和使用所述重组微生物产生各种有益代谢物的方法。 在本发明的各个方面，重组微生物可以进一步包含一种或多种修饰，导致还原或消除3个酮酸（例如乙酰乳酸和2-乙酰-2-羟基丁酸酯）和/或醛衍生的副产物 。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US08133715B2
公开(公告)日：2012-03-13
申请号：US13074907
申请日：2011-03-29
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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公开(公告)号：US20110275129A1
公开(公告)日：2011-11-10
申请号：US13074907
申请日：2011-03-29
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含生物合成途径的重组微生物和使用所述重组微生物产生各种有益代谢物的方法。 在本发明的各个方面，重组微生物可以进一步包含一种或多种修饰，导致还原或消除3个酮酸（例如乙酰乳酸和2-乙酰-2-羟基丁酸酯）和/或醛衍生的副产物 。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US20110201073A1
公开(公告)日：2011-08-18
申请号：US13077170
申请日：2011-03-31
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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6.发明申请CYTOSOLIC ISOBUTANOL PATHWAY LOCALIZATION FOR THE PRODUCTION OF ISOBUTANOL 审中-公开CYPOSOLIC ISOBUTANOL路线本地化生产ISOBUTANOL公开(公告)号：US20110287500A1
公开(公告)日：2011-11-24
申请号：US13176452
申请日：2011-07-05
申请人： Jun URANO , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Ruth Berry , Ishmeet Kalra
发明人： Jun URANO , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Ruth Berry , Ishmeet Kalra
CPC分类号： C12N15/81 , C12N9/0006 , C12N9/1022 , C12N9/88 , C12P7/16 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明提供重组微生物，其包含产生异丁醇的代谢途径与位于细胞溶质中的至少一种异丁醇途径酶，其中所述重组微生物被选择以从碳源产生异丁醇。 还提供了使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可以包含胞质活性异丁醇途径酶。 在一些实施方案中，本发明提供具有胞质活性的突变，修饰和/或嵌合异丁醇途径酶。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US20140295513A1
公开(公告)日：2014-10-02
申请号：US14131984
申请日：2012-07-11
申请人： Peter Meinhold , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Christopher Snow , Sabine Bastian , Sebastian Schoof
发明人： Peter Meinhold , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Christopher Snow , Sabine Bastian , Sebastian Schoof
CPC分类号： C12P7/16 , C12N9/0006 , C12Y101/01086 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 80% identical to SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 28, SEQ ID NO: 40, or SEQ ID NO: 58. The present invention also relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 99% identical to SEQ ID NO: 64. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.
摘要翻译： 本发明涉及包含编码酮酸酸还原异构酶（KARI）或修饰的NADH依赖性变体的至少一种核酸分子的重组微生物，其中所述KARI与SEQ ID NO：2，SEQ ID NO：2至少约80％ SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8，SEQ ID NO：10，SEQ ID NO：28，SEQ ID NO：40或SEQ ID NO：58.本发明还涉及重组微生物 其包含至少一个编码酮酸酸还原异构酶（KARI）或其修饰的NADH依赖性变体的核酸分子，其中所述KARI与SEQ ID NO：64至少约99％相同。在本发明的各个方面， 重组微生物可以包含产生异丁醇的代谢途径，并可用于制备异丁醇的方法。
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公开(公告)号：US20140295512A1
公开(公告)日：2014-10-02
申请号：US14126606
申请日：2012-06-15
申请人： Thomas Buelter , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Peter Meinhold , Ruth Berry , Christopher Snow , Sabine Bastian
发明人： Thomas Buelter , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Peter Meinhold , Ruth Berry , Christopher Snow , Sabine Bastian
CPC分类号： C12P7/16 , C12N9/0006 , C12N15/52 , C12Y101/01086 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.
摘要翻译： 本发明涉及包含编码酮酸酸还原异构酶（KARI）或其修饰的NADH依赖性变体的至少一种核酸分子的重组微生物，其中所述KARI与SEQ ID NO：2至少约60％相同。 本发明的方面，重组微生物可包含产生异丁醇的代谢途径，可用于制备异丁醇的方法。
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9.发明申请METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS 审中-公开增加二羟酸脱水酶活性的方法，以提高燃料，化学品和氨基酸的生产公开(公告)号：US20120288910A1
公开(公告)日：2012-11-15
申请号：US13528106
申请日：2012-06-20
申请人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Lynne H. Albert
发明人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Lynne H. Albert
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。 在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。 在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。 在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US20110236942A1
公开(公告)日：2011-09-29
申请号：US13076284
申请日：2011-03-30
申请人： Andrew Hawkins , Stephanie Porter-Scheinman , Catherine Asleson Dundon , Aristos Aristidou , Doug Lies , Julie Kelly
发明人： Andrew Hawkins , Stephanie Porter-Scheinman , Catherine Asleson Dundon , Aristos Aristidou , Doug Lies , Julie Kelly
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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