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1.发明申请METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS 审中-公开增加二羟酸脱水酶活性的方法，以提高燃料，化学品和氨基酸的生产公开(公告)号：US20120288910A1
公开(公告)日：2012-11-15
申请号：US13528106
申请日：2012-06-20
申请人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Lynne H. Albert
发明人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Lynne H. Albert
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。 在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。 在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。 在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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2.发明申请CYTOSOLIC ISOBUTANOL PATHWAY LOCALIZATION FOR THE PRODUCTION OF ISOBUTANOL 审中-公开CYPOSOLIC ISOBUTANOL路线本地化生产ISOBUTANOL公开(公告)号：US20110287500A1
公开(公告)日：2011-11-24
申请号：US13176452
申请日：2011-07-05
申请人： Jun URANO , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Ruth Berry , Ishmeet Kalra
发明人： Jun URANO , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Ruth Berry , Ishmeet Kalra
CPC分类号： C12N15/81 , C12N9/0006 , C12N9/1022 , C12N9/88 , C12P7/16 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明提供重组微生物，其包含产生异丁醇的代谢途径与位于细胞溶质中的至少一种异丁醇途径酶，其中所述重组微生物被选择以从碳源产生异丁醇。 还提供了使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可以包含胞质活性异丁醇途径酶。 在一些实施方案中，本发明提供具有胞质活性的突变，修饰和/或嵌合异丁醇途径酶。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US20110076733A1
公开(公告)日：2011-03-31
申请号：US12855276
申请日：2010-08-12
申请人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Ruth Berry , Ishmeet Kalra
发明人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Ruth Berry , Ishmeet Kalra
CPC分类号： C12N15/81 , C12N9/0006 , C12N9/1022 , C12N9/88 , C12P7/16 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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4.发明申请Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids 审中-公开酵母微生物减少副产物积累，以改善燃料，化学品和氨基酸的生产公开(公告)号：US20110201090A1
公开(公告)日：2011-08-18
申请号：US13025801
申请日：2011-02-11
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Kent Evans , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Kent Evans , Julie Kelly , Ruth Berry
IPC分类号： C12N1/00
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含生物合成途径的重组微生物和使用所述重组微生物产生各种有益代谢物的方法。 在本发明的各个方面，重组微生物可以进一步包含一种或多种修饰，导致还原或消除3个酮酸（例如乙酰乳酸和2-乙酰-2-羟基丁酸酯）和/或醛衍生的副产物 。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US08153415B2
公开(公告)日：2012-04-10
申请号：US13077170
申请日：2011-03-31
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含生物合成途径的重组微生物和使用所述重组微生物产生各种有益代谢物的方法。 在本发明的各个方面，重组微生物可以进一步包含一种或多种修饰，导致还原或消除3个酮酸（例如乙酰乳酸和2-乙酰-2-羟基丁酸酯）和/或醛衍生的副产物 。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US20110201073A1
公开(公告)日：2011-08-18
申请号：US13077170
申请日：2011-03-31
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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公开(公告)号：US08133715B2
公开(公告)日：2012-03-13
申请号：US13074907
申请日：2011-03-29
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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公开(公告)号：US20110275129A1
公开(公告)日：2011-11-10
申请号：US13074907
申请日：2011-03-29
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Matthew Peters , Melissa Dey , Justas Jancauskas , Julie Kelly , Ruth Berry
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含生物合成途径的重组微生物和使用所述重组微生物产生各种有益代谢物的方法。 在本发明的各个方面，重组微生物可以进一步包含一种或多种修饰，导致还原或消除3个酮酸（例如乙酰乳酸和2-乙酰-2-羟基丁酸酯）和/或醛衍生的副产物 。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US20080057577A1
公开(公告)日：2008-03-06
申请号：US11697404
申请日：2007-04-06
申请人： Frances Arnold , Peter Meinhold , Matthew Peters , Rudi Fasan , Mike Chen
发明人： Frances Arnold , Peter Meinhold , Matthew Peters , Rudi Fasan , Mike Chen
CPC分类号： C12P7/04 , C07K14/32 , C12N9/0042 , C12N9/0071 , C12N9/0077 , C12P7/06 , C12Y106/02004 , C12Y114/14001 , C12Y114/15 , C12Y114/15003 , Y02P20/52
摘要： This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
摘要翻译： 本发明涉及改性羟化酶。 本发明还涉及表达这种修饰的羟化酶的细胞和通过使合适的底物与这种细胞接触来制备羟基化烷烃的方法。
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10.发明申请Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 有权修饰的细胞色素P450的区域和对映选择性烷烃羟基化公开(公告)号：US20050059128A1
公开(公告)日：2005-03-17
申请号：US10869825
申请日：2004-06-15
申请人： Frances Arnold , Matthew Peters , Peter Meinhold
发明人： Frances Arnold , Matthew Peters , Peter Meinhold
IPC分类号： C07H21/04 , C12N20060101 , C12N9/02
CPC分类号： C12N9/0071 , C12N9/0077 , C12P7/04 , C12Y114/14001
摘要： Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.
摘要翻译： 使用定向进化和定点诱变的组合将来自巨大芽孢杆菌的细胞色素P450 BM-3设计为使用大气二氧氧化物作为区域和对映选择性的羟基化线型烷烃。 突变体9-10A-A328V主要在2-位羟基化辛烷值以形成S-2-辛醇（40％ee）。 另一个突变体1-12G，主要在2-位羟基化烷烃大于己烷，但形成R-2-醇（40-55％ee）。 这些生物催化剂对烷烃底物具有高活性，并支持数千种产品周转。 这些区域和对映体选择性保留在与大肠杆菌的全细胞生物转化中，其中工程化的P450可以以高水平表达并且昂贵的辅因子是内源性提供的。
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