公开(公告)号：US06538180B1

公开(公告)日：2003-03-25

申请号：US09214619

申请日：1999-07-15

申请人： Colin N. Lacey ,  Stephen G. Hughes ,  Christopher J. Harrison ,  Trevor L. Wang ,  Clifford L. Hedley

发明人： Colin N. Lacey ,  Stephen G. Hughes ,  Christopher J. Harrison ,  Trevor L. Wang ,  Clifford L. Hedley

IPC分类号： C12N1505

CPC分类号： C12N15/8245 ,  C12N9/92

摘要： Pea seeds, produced from peas of the rug3rug3 genotype, substantially lacking plastidial phosphoglucomutase activity which have higher sucrose levels at the end of the vining period and may be vined over and extended period compared to conventional vining varieties are provided. Polynucleootides encoding pea plastidial phosphoglucomutase useful for altering the sucrose and starch content of plants, particularly peas, are also disclosed.

摘要翻译： 豌豆种子由rug3rug3基因型的豌豆生产，基本上缺乏质量较差的磷酸果糖转移酶活性，其在酿造期结束时具有较高的蔗糖含量，并且与传统的酿造品种相比，可能会延长和延长时间。 还公开了可用于改变植物，特别是豌豆的蔗糖和淀粉含量的用于编码豌豆质粒磷酸蛋白酶的多核苷酸。

公开(公告)号：US5296365A

公开(公告)日：1994-03-22

申请号：US721409

申请日：1991-06-26

申请人： Nicholaas Overbeeke ,  Arthur Fellinger ,  Stephen G. Hughes

发明人： Nicholaas Overbeeke ,  Arthur Fellinger ,  Stephen G. Hughes

IPC分类号： A23K1/165 ,  A61K8/00 ,  A61K8/02 ,  A61K8/73 ,  C12N1/14 ,  C12N1/16 ,  C12N1/19 ,  C12N1/20 ,  C12N5/00 ,  C12N5/10 ,  C12N9/26 ,  C12N9/28 ,  C12N9/40 ,  C12N15/00 ,  C12N15/09 ,  C12N15/56 ,  C12N15/75 ,  C12N15/81 ,  C12N15/82 ,  C12P19/14 ,  C12N15/74 ,  C12N15/80

CPC分类号： C12N9/2408 ,  C12N15/75 ,  C12N15/81 ,  C12N15/815 ,  C12N15/8242 ,  C12N15/8257 ,  C12N9/2417 ,  C12N9/2465 ,  C07K2319/02

摘要： The gene encoding an alpha-galactosidase from guar seed has been isolated and identified. It has been expressed in yeasts, e.g. Saccharomyces, Kluyveromyces and Hansenula, in bacteria, e.g. Bacillus, and in plants, e.g. Nicotiana. The resulting alpha-galactosidase is able to decrease the galactose content of galactomannans such a guar gum by splitting off 1-6 linked alpha-D-galactopyranosyl units attached to a main chain of 1-4 lined beta-mannopyranosyl units. Thus a commercially feasible process becomes available for producing alpha-galactosidases suitable for providing galactomannans having improved properties, which can meet the increasing demand for good quality polysaccharides. It has further been demonstrated that other alphagalactosidases, which show a positive immunological cross-reaction with an antibody raised against the alpha-galactosidase from guar, are also effective in decreasing the galactose content of galactomannans such as guar.

摘要翻译： 已经分离并鉴定了编码来自瓜尔胶种子的α-半乳糖苷酶的基因。 已在酵母中表达，例如 酵母属，克鲁维酵母和汉逊酵母，细菌，例如。 芽孢杆菌属和植物中。 烟草 所得的α-半乳糖苷酶能够通过分离连接到1-4衬里的β-甘露吡喃糖基单元的主链上的1-6个连接的α-D-吡喃半乳糖基单元来降低半乳甘露聚糖如瓜尔胶的半乳糖含量。 因此，商业可行的方法可用于生产适于提供具有改善性质的半乳甘露聚糖的α-半乳糖苷酶，其可以满足对优质多糖的日益增长的需求。 进一步证明，与来自瓜尔胶的α-半乳糖苷酶产生的抗体显示出阳性的免疫交叉反应的其他α-角苷酶也有效降低半乳甘露聚糖（例如瓜尔胶）的半乳糖含量。

公开(公告)号：US07034203B1

公开(公告)日：2006-04-25

申请号：US09626377

申请日：1999-01-25

申请人： Arnaud G. Bovy ,  Hendrikus T. van der Hijden ,  Stephen G. Hughes ,  Shelagh R. Muir ,  Adrianus J. van Tunen ,  Martine E. Verhoeyen ,  Cornelis H. de Vos

发明人： Arnaud G. Bovy ,  Hendrikus T. van der Hijden ,  Stephen G. Hughes ,  Shelagh R. Muir ,  Adrianus J. van Tunen ,  Martine E. Verhoeyen ,  Cornelis H. de Vos

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/08 ,  A01H5/10

CPC分类号： C07K14/415 ,  A23L19/03 ,  A23L19/09 ,  A23L33/105 ,  C12N15/8243 ,  C12N15/825

摘要： A method for manipulating the production of flavonoids other than anthocyanins in plants by manipulating gene activity in the flavonoid biosynthetic pathway by expressing two or more genes encoding transcription factors for flavonoid biosynthesis, compositions for use in such a method and tomato plants having altered flavonoid levels are disclosed.

摘要翻译： 通过表达两种以上编码黄酮生物合成转录因子的基因和用于这种方法的组合物以及具有改变的类黄酮水平的番茄植物，通过操作类黄酮生物合成途径中的基因活性来操作植物中除花青素以外的类黄酮的生产方法 披露

公开(公告)号：US5082778A

公开(公告)日：1992-01-21

申请号：US165995

申请日：1988-02-02

申请人： Nicolaas Overbeeke ,  Arthur J. Fellinger ,  Stephen G. Hughes

发明人： Nicolaas Overbeeke ,  Arthur J. Fellinger ,  Stephen G. Hughes

IPC分类号： A23K1/165 ,  A61K8/00 ,  A61K8/02 ,  A61K8/73 ,  C12N1/14 ,  C12N1/16 ,  C12N1/19 ,  C12N1/20 ,  C12N5/00 ,  C12N5/10 ,  C12N9/26 ,  C12N9/28 ,  C12N9/40 ,  C12N15/00 ,  C12N15/09 ,  C12N15/56 ,  C12N15/75 ,  C12N15/81 ,  C12N15/82 ,  C12P19/14

CPC分类号： C12N9/2408 ,  C12N15/75 ,  C12N15/81 ,  C12N15/815 ,  C12N15/8242 ,  C12N15/8257 ,  C12N9/2417 ,  C12N9/2465 ,  C07K2319/02

摘要： The gene encoding an alpha-galactosidase from guar seed has been isolated and identified. It has been expressed in yeasts, e.g. Saccharomyces, Kluyveromyces and Hansenula, in bacteria, e.g. Bacillus, and in plants, e.g. Nicotiana. The resulting alpha-galactosidase is able to decrease the galactose content of galactomannans such as guar gum by splitting off 1-6 linked alpha-D-galactopyranosyl units attached to a main chain of 1-4 lined beta-mannopyranosyl units. Thus a commercially feasible process becomes available for producing alpha-galactosidases suitable for providing galactomannans having improved properties, which can meet the increasing demand for good quality polysaccharides. It has further been demonstrated that other alphagalactosidases, which show a positive immunological cross-reaction with an antibody raised against the alpha-galactosidase with an antibody raised against the alpha-galactosidase from guar, are also effective in decreasing the galactose content of galactomannans such as guar.