公开(公告)号：US06262333B1

公开(公告)日：2001-07-17

申请号：US09328111

申请日：1999-06-08

申请人： Wilson O. Endege ,  Kathleen E. Steinmann ,  Jon H. Astle ,  Christopher C. Burgess ,  Steven E. Bushnell ,  Eddie Carroll, III ,  Theodore J. Catino ,  Adnan Derti ,  Donna M. Ford ,  Marcia E. Lewis ,  John E. Monahan ,  Robert Schlegel

发明人： Wilson O. Endege ,  Kathleen E. Steinmann ,  Jon H. Astle ,  Christopher C. Burgess ,  Steven E. Bushnell ,  Eddie Carroll, III ,  Theodore J. Catino ,  Adnan Derti ,  Donna M. Ford ,  Marcia E. Lewis ,  John E. Monahan ,  Robert Schlegel

IPC分类号： A01K6700

CPC分类号： C07K14/47 ,  A61K38/00

摘要： This invention relates to novel human genes, to proteins expressed by the genes, and to variants of the proteins. The invention also relates to diagnostic assays and therapeutic agents related to the genes and proteins, including probes, antisense constructs. and antibodies. The subject nucleic acids have been found to be differentially regulated in tumor cells, particularly colon cancer cell lines and/or tissue.

摘要翻译： 本发明涉及新的人类基因，由基因表达的蛋白质以及蛋白质的变体。 本发明还涉及与基因和蛋白质相关的诊断测定和治疗剂，包括探针，反义构建体。 和抗体。 已经发现目标核酸在肿瘤细胞，特别是结肠癌细胞系和/或组织中是差异调节的。

公开(公告)号：US5888835A

公开(公告)日：1999-03-30

申请号：US644909

申请日：1996-05-10

申请人： Steven E. Bushnell ,  Tina K. Garyantes ,  Michael L. Malek ,  Howard J. Kirchick

发明人： Steven E. Bushnell ,  Tina K. Garyantes ,  Michael L. Malek ,  Howard J. Kirchick

IPC分类号： B03C1/28 ,  G01N33/543 ,  G01N33/553 ,  G01N35/00

CPC分类号： G01N33/54326 ,  B03C1/288 ,  G01N35/0098 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/113332

摘要： Method and apparatus for enabling resuspension wash and magnetic localization of sample components bound to particles with magnetic properties in reaction vessels during separation and wash for enhanced chemiluminescent signal generation in biomedical assays. The assays involve moving reaction vessels past magnetic arrays that partially localized the particles prior to passing a gap where washing occurs, with or without resuspension, after separating out the unbound components and liquid. The band of particles is further localized by a focusing magnet at the end of the array prior to dosing the vessel with acid for chemiluminescent purposes. A block of soft magnetic material is employed in place of a magnet in the gap to minimize magnetic strength at the gap. Trimmed magnets adjacent the gap cause left, then right, particle shifting that localizes the magnetizable particles. The gap enables improved resuspension by wash, whereas the localized particles enable more efficient resuspension by reagent.

摘要翻译： 用于在分离和洗涤期间使得能够重新悬浮洗涤和磁性定位结合到反应容器中的具有磁性的颗粒的样品组分的洗涤和磁定位的方法和装置，用于生物医学测定中增强的化学发光信号产生。 测定包括使分离出未结合的组分和液体之后，将反应容器移动通过磁性阵列，该磁性阵列在通过发生洗涤的间隙之前部分地定位该颗粒，同时具有或不具有重新悬浮。 在用酸化学发光目的给容器给料之前，颗粒带在进一步定位在阵列末端的聚焦磁体。 使用一块软磁性材料代替间隙中的磁体，以使间隙处的磁强度最小化。 邻近间隙的修整磁体会导致左边，然后是右边的颗粒移动，使可磁化的颗粒定位。 该间隙能够通过洗涤改善再悬浮，而局部颗粒使得能够通过试剂更有效的再悬浮。