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    Sensitive immunoassays utilizing antibody conjugates with replicable DNA templates
    4.
    发明授权
    Sensitive immunoassays utilizing antibody conjugates with replicable DNA templates 失效
    使用具有可复制DNA模板的抗体缀合物的敏感免疫测定

    公开(公告)号：US06083689A

    公开(公告)日：2000-07-04

    申请号：US416571

    申请日：1995-04-04

    申请人： Richard A. Martinelli ,  Eddie Carroll, III

    发明人： Richard A. Martinelli ,  Eddie Carroll, III

    IPC分类号： G01N33/532 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/58 ,  G01N33/53 ,  G01N33/536

    CPC分类号： G01N33/581 ,  C12Q1/6804 ,  C12Q1/682 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/824 ,  Y10S436/828

    摘要： A novel signal amplification system for immunoassays that minimizes non-specific signals is disclosed. Immunoassay methods, reagents and test kits are described for obtaining immunoassays of enhanced sensitivity. The reagents include antibody-variant DNA conjugates, wherein the variant DNA is a substrate for an RNA-dependent RNA polymerase, such as, QB replicase. Immunoassay methods to detect, or to detect and quantitate, analyte in test samples comprise transcribing the variant DNA of said antibody-DNA conjugates that are bound to analyte, to RNA, and replicating the RNA transcripts, wherein the presence or quantity of variant RNA replication products can be correlated with the presence or quantity of analyte in the test samples. Further, methods are provided to detect, or to detect and quantitate, simultaneously two or more analytes in a test sample.

    摘要翻译： 公开了一种用于使非特异性信号最小化的免疫测定的新型信号放大系统。 描述了用于获得增强灵敏度的免疫测定的免疫测定方法，试剂和测试试剂盒。 试剂包括抗体变体DNA缀合物，其中变体DNA是用于RNA依赖性RNA聚合酶的底物，例如QB复制酶。 用于检测或定量测试样品中的分析物的免疫测定方法包括将与分析物结合的所述抗体-DNA缀合物的变体DNA转录成RNA并复制RNA转录物，其中变体RNA复制物的存在或数量 产品可以与测试样品中分析物的存在或数量相关联。 此外，提供了用于同时检测或定量测试样品中两种或更多种分析物的方法。

    Non-separation specific binding chemiluminescent assay
    5.
    发明授权
    Non-separation specific binding chemiluminescent assay 失效
    非分离特异性结合化学发光测定

    公开(公告)号：US5783453A

    公开(公告)日：1998-07-21

    申请号：US496549

    申请日：1995-06-29

    申请人： Eve H. Barlow ,  Eddie Carroll, III ,  Joseph E. Connolly ,  Michael J. Lee ,  Richard A. Martinelli ,  John T. Unger

    发明人： Eve H. Barlow ,  Eddie Carroll, III ,  Joseph E. Connolly ,  Michael J. Lee ,  Richard A. Martinelli ,  John T. Unger

    IPC分类号： G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  C12G1/00 ,  G01N33/551 ,  G01N33/553

    CPC分类号： G01N33/542 ,  G01N33/582

    摘要： The assay described herein is predicated on an observation that when acridinium ester labeled tracers are bound to their corresponding binding conjugate immobilized on a metal oxide solid phase, the measurable chemiluminescent light emission of the labeled tracer bound to the solid phase is quenched as compared to the free fraction tracer that is unattached to the solid phase. According to the invention, a non-separation specific binding assay to detect or quantify the presence of an analyte is provided where the entire reaction mixture is flashed (including unreacted tracer) and modulated signal (because of the quench effect) is associated with a reference, thus determining the amount or presence of said analyte in said sample. Disadvantages inherent in heterogeneous assays employing multiple separations may be avoided using this non-separation method.

    摘要翻译： 本文所述的测定是基于以下观察结果：当吖啶酯标记的示踪剂与其固定在金属氧化物固相上的相应结合缀合物结合时，与固相结合的标记示踪剂的可测量化学发光光发射与 与固相无关的游离级分示踪剂。 根据本发明，提供了检测或定量分析物存在的非分离特异性结合测定法，其中整个反应混合物闪蒸（包括未反应的示踪剂）和调制信号（由于骤冷作用）与参考文献相关联 ，从而确定所述样品中所述分析物的量或存在。 使用这种非分离方法可以避免使用多次分离的异质测定中固有的缺点。