公开(公告)号：US07662943B2

公开(公告)日：2010-02-16

申请号：US11721515

申请日：2005-12-16

申请人： Young-Hoon Park ,  Hyun-Soo Kim ,  Hye-Jin Choi ,  Jin-Ho Lee ,  Soo-Youn Hwang ,  Jae-Ick Sim ,  Tae-Sun Kang ,  Won-Sik Lee

发明人： Young-Hoon Park ,  Hyun-Soo Kim ,  Hye-Jin Choi ,  Jin-Ho Lee ,  Soo-Youn Hwang ,  Jae-Ick Sim ,  Tae-Sun Kang ,  Won-Sik Lee

IPC分类号： C12N15/11 ,  C12N15/63 ,  C12N15/70 ,  C12N1/20

CPC分类号： C07K14/34

摘要： Provided are a promoter including at least one polynucleotide selected from the group consisting of SEQ ID NOS: 1 to 7, an expression cassette including the same, a vector including the expression cassette, a host cell including the vector, and a method of expressing a gene using the host cell.

摘要翻译： 提供了包含选自SEQ ID NO：1至7的至少一种多核苷酸的启动子，包含其的表达盒，包含表达盒的载体，包含载体的宿主细胞，以及表达 基因使用宿主细胞。

公开(公告)号：US08669078B2

公开(公告)日：2014-03-11

申请号：US12097104

申请日：2006-12-14

申请人： Jae-Gu Pan ,  Heung-Chae Jung ,  Eui-Joong Kim ,  Han-Seung Lee ,  Young Hoon Park ,  Hyoung Suk Kim ,  Jong-Kwon Han ,  Jin Nam Lee ,  Ki-Hoon Oh ,  Jeong Hwan Kim ,  Yoon-Suk Oh ,  Jae Ick Sim ,  Kuk-Ki Hong ,  Kyung Oh Choi ,  Hyun Soo Kim ,  Min-Ji Baek ,  Tae Sun Kang

发明人： Jae-Gu Pan ,  Heung-Chae Jung ,  Eui-Joong Kim ,  Han-Seung Lee ,  Young Hoon Park ,  Hyoung Suk Kim ,  Jong-Kwon Han ,  Jin Nam Lee ,  Ki-Hoon Oh ,  Jeong Hwan Kim ,  Yoon-Suk Oh ,  Jae Ick Sim ,  Kuk-Ki Hong ,  Kyung Oh Choi ,  Hyun Soo Kim ,  Min-Ji Baek ,  Tae Sun Kang

IPC分类号： C12P19/40 ,  C12P21/06 ,  C12N15/00 ,  C07H21/02

CPC分类号： C12N9/78 ,  C12P19/32

摘要： Disclosed herein are ammonia-specific 5′-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5′-XMP aminase. The resulting 5′-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5′-XMP into 5′-GMP. Thus, the ammonia-specific 5′-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5′-XMP into 5′-GMP.

摘要翻译： 本文公开了氨特异性5'-XMP氨基酶突变体及其制备方法。 在5'-XMP氨基酶中引入谷氨酰胺依赖性催化活性位点的突变。 所得到的5'-XMP氨基酶突变体缺乏谷氨酰胺依赖性活性，并特异性地与外部氨反应将5'-XMP转化为5'-GMP。 因此，与野生型相比，氨特异性5'-XMP氨基酶突变体在细胞内更稳定，可用于将5'-XMP工业转化为5'-GMP。

公开(公告)号：US20080318278A1

公开(公告)日：2008-12-25

申请号：US12097104

申请日：2006-12-14

申请人： Jae-Gu Pan ,  Heung-Chae Jung ,  Eui-Joong Kim ,  Han-Seung Lee ,  Young Hoon Park ,  Hyoung Suk Kim ,  Jong-Kwon Han ,  Jin Nam Lee ,  Ki-Hoon Oh ,  Jeong Hwan Kim ,  Yoon-Suk Oh ,  Jae Ick Sim ,  Kuk-Ki Hong ,  Kyung Oh Choi ,  Hyun Soo Kim ,  Min-Ji Baek ,  Tae Sun Kang

发明人： Jae-Gu Pan ,  Heung-Chae Jung ,  Eui-Joong Kim ,  Han-Seung Lee ,  Young Hoon Park ,  Hyoung Suk Kim ,  Jong-Kwon Han ,  Jin Nam Lee ,  Ki-Hoon Oh ,  Jeong Hwan Kim ,  Yoon-Suk Oh ,  Jae Ick Sim ,  Kuk-Ki Hong ,  Kyung Oh Choi ,  Hyun Soo Kim ,  Min-Ji Baek ,  Tae Sun Kang

IPC分类号： C12P19/30 ,  C12N9/10 ,  C07H21/04 ,  C07H21/02 ,  C12N15/64 ,  C12N1/21

CPC分类号： C12N9/78 ,  C12P19/32

摘要： Disclosed herein are ammonia-specific 5′-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5′-XMP aminase. The resulting 5′-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5′-XMP into 5′-GMP. Thus, the ammonia-specific 5′-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5′-XMP into 5′-GMP.

摘要翻译： 本文公开了氨特异性5'-XMP氨基酶突变体及其制备方法。 在5'-XMP氨基酶中引入谷氨酰胺依赖性催化活性位点的突变。 所得到的5'-XMP氨基酶突变体缺乏谷氨酰胺依赖性活性，并特异性地与外部氨反应将5'-XMP转化为5'-GMP。 因此，与野生型相比，氨特异性5'-XMP氨基酶突变体在细胞内更稳定，可用于将5'-XMP工业转化为5'-GMP。

公开(公告)号：US11717878B2

公开(公告)日：2023-08-08

申请号：US17430656

申请日：2019-11-24

申请人： Tae Sun Kang

发明人： Tae Sun Kang ,  GeunHo Nam

IPC分类号： B21J15/22 ,  B21J15/28

CPC分类号： B21J15/22 ,  B21J15/28

摘要： The present disclosure relates to a device for fastening a lockbolt, the device comprising: a lockbolt gun including a body unit having a grip therein for pulling the lockbolt and a gripping unit protruding from a lower portion of the body unit and having an operation switch installed on the outside, wherein a hydraulic supply flow path for supplying hydraulic pressure for driving the grip, a hydraulic discharge flow path for discharging hydraulic pressure supplied to the grip, and an air supply flow path for supplying pneumatic pressure to the operation switch are installed in the gripping unit, and a discharge hole is formed outside the gripping unit to discharge air from the air supply flow path when the operation switch is operated; a driving force supply unit having a hydraulic supply pump that supplies hydraulic pressure to the hydraulic supply flow path, a hydraulic recovery pump that recovers hydraulic pressure discharged from the hydraulic discharge flow path, and an oil tank that supplies oil to the hydraulic supply pump and recovers oil from the hydraulic recovery pump; an operating unit having a first solenoid valve connected to the hydraulic supply pump, the hydraulic recovery pump, and the air supply flow path, an air supply unit that supplies air to the first solenoid valve, and an air pressure regulator installed between the first solenoid valve and the air supply unit to regulate pneumatic pressure supplied to the first solenoid valve; a housing accommodating the driving force supply unit and the operating unit; and a control unit for controlling the hydraulic supply pump and the hydraulic recovery pump to operate so that the pneumatic pressure is supplied from the first solenoid valve to the hydraulic supply pump and the hydraulic recovery pump when an operating signal is inputted from the operation switch.

公开(公告)号：US20220118504A1

公开(公告)日：2022-04-21

申请号：US17430656

申请日：2019-11-24

申请人： Tae Sun KANG

发明人： Tae Sun KANG ,  GeunHo NAM

IPC分类号： B21J15/22 ,  B21J15/28

摘要： The present disclosure relates to a device for fastening a lockbolt, the device comprising: a lockbolt gun including a body unit having a grip therein for pulling the lockbolt and a gripping unit protruding from a lower portion of the body unit and having an operation switch installed on the outside, wherein a hydraulic supply flow path for supplying hydraulic pressure for driving the grip, a hydraulic discharge flow path for discharging hydraulic pressure supplied to the grip, and an air supply flow path for supplying pneumatic pressure to the operation switch are installed in the gripping unit, and a discharge hole is formed outside the gripping unit to discharge air from the air supply flow path when the operation switch is operated; a driving force supply unit having a hydraulic supply pump that supplies hydraulic pressure to the hydraulic supply flow path, a hydraulic recovery pump that recovers hydraulic pressure discharged from the hydraulic discharge flow path, and an oil tank that supplies oil to the hydraulic supply pump and recovers oil from the hydraulic recovery pump; an operating unit having a first solenoid valve connected to the hydraulic supply pump, the hydraulic recovery pump, and the air supply flow path, an air supply unit that supplies air to the first solenoid valve, and an air pressure regulator installed between the first solenoid valve and the air supply unit to regulate pneumatic pressure supplied to the first solenoid valve; a housing accommodating the driving force supply unit and the operating unit; and a control unit for controlling the hydraulic supply pump and the hydraulic recovery pump to operate so that the pneumatic pressure is supplied from the first solenoid valve to the hydraulic supply pump and the hydraulic recovery pump when an operating signal is inputted from the operation switch.

公开(公告)号：US20080138859A1

公开(公告)日：2008-06-12

申请号：US11721515

申请日：2005-12-16

申请人： Young-Hoon Park ,  Hyun-Soo Kim ,  Hye-Jin Choi ,  Jin-Ho Lee ,  Soo-Youn Hwang ,  Jae-Ick Sim ,  Tae-Sun Kang ,  Won-Sik Lee

发明人： Young-Hoon Park ,  Hyun-Soo Kim ,  Hye-Jin Choi ,  Jin-Ho Lee ,  Soo-Youn Hwang ,  Jae-Ick Sim ,  Tae-Sun Kang ,  Won-Sik Lee

IPC分类号： C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12N1/20

CPC分类号： C07K14/34

摘要： Provided are a promoter including at least one polynucleotide selected from the group consisting of SEQ ID NOS: 1 to 7, an expression cassette including the same, a vector including the expression cassette, a host cell including the vector, and a method of expressing a gene using the host cell.

摘要翻译： 提供了包含选自SEQ ID NO：1至7的至少一种多核苷酸的启动子，包含其的表达盒，包含表达盒的载体，包含载体的宿主细胞，以及表达 基因使用宿主细胞。