公开(公告)号：US08218132B2

公开(公告)日：2012-07-10

申请号：US12595348

申请日：2008-04-08

申请人： Yukio Yamada ,  Shinpei Okawa ,  Taisuke Hirono

发明人： Yukio Yamada ,  Shinpei Okawa ,  Taisuke Hirono

IPC分类号： G01P3/36

CPC分类号： G01N15/1463 ,  G01N15/1484 ,  G01N2015/1075 ,  G01N2015/1486 ,  G01N2015/1493

摘要： Particles flowed through a micro-channel are imaged by imaging means. Particle speed measuring means determines the particle speed from the image data. Particle counting means counts the particles flowed for a predetermined time. Particle size measuring means measures the size of the particles. The measurements of the particles flowed at a predetermined timing are managed by data associating means. With this, the speed, number and size of particles flowed through a micro-channel can be determined at a time, and associated data can be obtained.

摘要翻译： 流过微通道的颗粒通过成像装置成像。 粒子速度测量装置根据图像数据确定粒子速度。 颗粒计数装置计算流过预定时间的颗粒。 粒度测量装置测量颗粒的大小。 在预定定时流动的颗粒的测量由数据关联装置管理。 因此，可以一次确定流过微通道的粒子的速度，数量和尺寸，并且可以获得相关数据。

公开(公告)号：US20100279393A1

公开(公告)日：2010-11-04

申请号：US12698425

申请日：2010-02-02

申请人： Taisuke Hirono

发明人： Taisuke Hirono

IPC分类号： C12M1/34

CPC分类号： B01L3/502776 ,  B01L2300/0867 ,  B01L2400/0418 ,  B01L2400/0487

摘要： In FIG. 2(a), both laminar flows, solution including fine grains and buffer solution, are supplied in a microchannel in a micro chip device, and gel including drug is supplied at a position contacting with the buffer solution. The fine grains in the solution including fine grains starts to react after contacting with the gel including drug, but the reaction does not start since the drug is separated from the buffer solution in FIG. 2 (c). If supply of the buffer solution is stopped in the above-mentioned state, the solution including fine grains and the gel including drug are contacted and the reaction between the fine grains and the drug can be observed at a fixed point. In such a device, it is sufficient to supply the gel including drug from the supply passage without coating the drug on a wall face of the microchannel and the drug coating operation can be omitted thereby.

摘要翻译： 在图 如图2（a）所示，在微芯片装置的微通道中供给层流，包含细晶粒和缓冲液的溶液，在与缓冲溶液接触的位置供给含有药物的凝胶。 包含细晶粒的溶液中的细晶粒在与包含药物的凝胶接触后开始反应，但是由于药物与图1中的缓冲溶液分离，反应不起始。 2（c）。 如果在上述状态下停止供给缓冲溶液，则包含细粒和包含药物的凝胶的溶液接触，可以在固定点观察细粒和药物之间的反应。 在这种装置中，从供给通道供给包含药物的凝胶，而不将药物涂布在微通道的壁面上，从而可以省略药物涂敷操作。

公开(公告)号：US08740384B2

公开(公告)日：2014-06-03

申请号：US13501321

申请日：2010-09-22

申请人： Taisuke Hirono

发明人： Taisuke Hirono

IPC分类号： A61B3/10 ,  A61B3/00 ,  A61B5/00

CPC分类号： A61B3/10 ,  G01N21/39 ,  G01N21/51 ,  G01N2021/399

摘要： A method and apparatus for measuring a molecular composition of protein molecules. A fluid containing at least two varieties of protein molecules is irradiated with first and second laser light beams having different wavelengths. The molecular composition of the at least two varieties of protein molecules contained in the fluid is measured on the basis of an autocorrelation function of a scattered light signal from the at least two varieties of protein molecules irradiated with the first and second laser light beams having different wavelengths.

摘要翻译： 用于测量蛋白质分子的分子组成的方法和装置。 含有至少两种蛋白质分子的流体被具有不同波长的第一和第二激光束照射。 流体中含有的蛋白质分子中至少两种蛋白质分子的分子组成基于来自至少两种不同种类的蛋白质分子的散射光信号的自相关函数来测量，所述蛋白质分子用具有不同的第一和第二激光束照射 波长。

公开(公告)号：US20120194785A1

公开(公告)日：2012-08-02

申请号：US13501321

申请日：2010-09-22

申请人： Taisuke Hirono

发明人： Taisuke Hirono

IPC分类号： A61B3/10 ,  G01N21/49

CPC分类号： A61B3/10 ,  G01N21/39 ,  G01N21/51 ,  G01N2021/399

摘要： The inside of an anterior chamber (21) of a human eye containing the protein molecules of albumin and globulin is irradiated with laser light beams having different wavelengths from a variable-wavelength laser light source (10). Scattered light from the protein molecules is received by a detector (8), and an autocorrelation function of the scattered light signal is determined by a correlator (9). A computer (11) computes the concentrations of albumin and globulin or the ratio thereof on the basis of the autocorrelation functions of the scattered light signals of the albumin and globulin irradiated with the laser light beams having different wavelengths. In such a configuration, the concentrations of albumin and globulin, or the ratio thereof can be measured reliably without contact and in a noninvasive manner.

摘要翻译： 包含白蛋白和球蛋白的蛋白质分子的人眼前房（21）的内部用来自可变波长激光光源（10）的不同波长的激光照射。 通过检测器（8）接收来自蛋白质分子的散射光，由相关器（9）确定散射光信号的自相关函数。 计算机（11）基于用具有不同波长的激光照射的白蛋白和球蛋白的散射光信号的自相关函数计算白蛋白和球蛋白的浓度或其比例。 在这样的结构中，白蛋白和球蛋白的浓度或其比例可以在不接触的情况下以非侵入的方式被可靠地测量。

公开(公告)号：US07295306B2

公开(公告)日：2007-11-13

申请号：US11106776

申请日：2005-04-15

申请人： Taisuke Hirono

发明人： Taisuke Hirono

IPC分类号： G01N1/10

CPC分类号： B01F5/0646 ,  B01F5/0647 ,  B01F13/0059 ,  B01L3/502776 ,  B01L2200/10 ,  B01L2300/0867 ,  G01N15/1468 ,  G01N21/6428 ,  G01N2015/1486

摘要： When connecting a blood collecting unit (not shown) with an adaptor, streaming blood in a first inflow passage, and streaming liquid including surfactant or fluorescent dyes in a second inflow passage, the blood and the liquid are mixed at a mixing portion, and platelets, erythrocytes and cell membranes of leukocytes in the blood are dissolved by the surfactant, and the nuclei of the leukocytes are fluorescently stained with the fluorescent dyes. Then, a fluorescent particle counting portion counts the fluorescently stained nuclei of the leukocytes during moving in an outflow passage. According to the invention, a number of leukocytes can be correctly measured with a simple operation, such as connection of a blood collecting unit or the like with the adaptor.

摘要翻译： 当将血液收集单元（未示出）与适配器连接时，在第一流入通道中流动血液，以及在第二流入通道中包含表面活性剂或荧光染料的流动液体，血液和液体在混合部分混合，并且血小板 血液中的白细胞的红细胞和细胞膜被表面活性剂溶解，白细胞的核被荧光染料荧光染色。 然后，在流出通道中移动时，荧光粒子计数部对白细胞的荧光染色核进行计数。 根据本发明，可以通过简单的操作来正确地测量多个白细胞，诸如将血液收集单元等与适配器的连接。

公开(公告)号：US20080056953A1

公开(公告)日：2008-03-06

申请号：US11751793

申请日：2007-05-22

申请人： Yukio Yamada ,  Naoto Kakuta ,  Taisuke Hirono

发明人： Yukio Yamada ,  Naoto Kakuta ,  Taisuke Hirono

IPC分类号： B01L3/02

CPC分类号： B01L3/502776 ,  B01L2200/0636 ,  B01L2200/143 ,  B01L2300/0816 ,  B01L2400/0487 ,  B01L2400/0622

摘要： Buffer solution and blood are streamed in a channel of a micro chip so as to form layers. Aggregation inducing agent for aggregating platelets in blood is coated at a wall face on a buffer solution streaming side. If streaming amount of blood is increased in this state, a layer width of blood can be increased, and detail analysis between the aggregation inducing agent and the platelets is possible thereby. Even if it is necessary to take an image or a moving image for comparison between a pre-aggregation state and an aggregation state, it is sufficient to take only a portion where the aggregation inducing agent is coated, that is, a reaction portion. Then, a device can be made cheaper without two cameras or a moving mechanism for the camera or a micro chip.

摘要翻译： 缓冲溶液和血液在微芯片的通道中流动以形成层。 用于聚集血液中血小板的聚集诱导剂在缓冲溶液流动侧的壁面上被涂覆。 如果在这种状态下血液流量增加，则可以增加血液层的宽度，从而能够进行聚集诱导剂与血小板的细节分析。 即使在预聚合状态和聚集状态之间需要拍摄图像或运动图像进行比较，只需要仅涂覆聚集诱导剂的部分即反应部分即可。 然后，如果没有两个相机或用于相机或微芯片的移动机构，则可以使设备更便宜。

公开(公告)号：US06719203B2

公开(公告)日：2004-04-13

申请号：US09901288

申请日：2001-07-09

申请人： Taisuke Hirono ,  Muneharu Ishikawa

发明人： Taisuke Hirono ,  Muneharu Ishikawa

IPC分类号： G06K710

CPC分类号： B01L3/5453 ,  B01L3/545 ,  G01N2035/00752 ,  Y10T436/11

摘要： Cuvettes in boxes for blood testing and the like, are to be distinguished by bar code cuvette identification codes on each cuvette, together with a box identification code. The cuvettes have very limited space for bar code characters. The cuvette bar code reader is responsive to at least two different kinds of control codes, i.e., two distinct start code values and/or stop code values. The distinct control codes operate the bar code reader, but also contribute to the information that is encoded. A cuvette identification code is developed that combines an information code value with distinctions among the control codes found, to increase the number of values that can be encoded. In one embodiment, four start codes, four stop codes and a single decimal information digit, provide 160 different values.

摘要翻译： 用于血液检测的盒子中的比色皿等将通过每个比色杯上的条形码比色皿识别代码以及盒子识别码进行区分。 比色杯的条形码字符空间非常有限。 比色皿条形码读取器响应于至少两种不同类型的控制代码，即两个不同的起始码值和/或停止码值。 不同的控制代码操作条形码读取器，但也有助于编码的信息。 开发了比色皿识别码，其将信息码值与找到的控制码之间的区别相结合，以增加可编码的值的数量。 在一个实施例中，四个起始码，四个停止码和一个十进制小数信息数字提供160个不同的值。

公开(公告)号：US06198540B1

公开(公告)日：2001-03-06

申请号：US09402023

申请日：1999-09-24

申请人： Mamoru Ueda ,  Taisuke Hirono ,  Kohji Ohbayashi ,  Itaru Yoshizawa

发明人： Mamoru Ueda ,  Taisuke Hirono ,  Kohji Ohbayashi ,  Itaru Yoshizawa

IPC分类号： G01B902

CPC分类号： G01N21/4795 ,  G01B9/02002 ,  G01B9/02007 ,  G01B9/02028 ,  G01B9/02091 ,  G01B2290/35

摘要： An optical measuring instrument which enables measurement of necessary data in a short period of time and uses a light with a short coherence length. In this instrument, such a light emitted from a light source is divided into a measurement light and a plurality of reference lights. The plurality of reference lights are processed by different frequency modulations, and then multiplexed with a light reflected from a measurement object irradiated by the measurement light. On the basis of an output of a photoelectric converter for detecting the level of the multiplexed light and the frequency of each reference light, optical characteristic data related to a plurality of measurement points are calculated.

摘要翻译： 一种光学测量仪器，能够在短时间内测量必要的数据并使用具有短相干长度的光。 在该仪器中，从光源发射的光被分为测量光和多个参考光。 多个参考光通过不同的频率调制进行处理，然后与由测量光照射的测量对象反射的光复用。 基于用于检测多路复用光的电平的光电转换器的输出和每个参考光的频率，计算与多个测量点相关的光学特性数据。

公开(公告)号：US5610672A

公开(公告)日：1997-03-11

申请号：US355515

申请日：1994-12-14

申请人： Taisuke Hirono ,  Kenkichi Ueda

发明人： Taisuke Hirono ,  Kenkichi Ueda

IPC分类号： A61B3/10 ,  A61B3/117

CPC分类号： A61B3/117

摘要： An ophthalmic measurement apparatus for measuring biological properties by scanning the anterior chamber of an eye two-dimensionally with a laser beam. Light received from the anterior chamber is measured at a plurality of measurement points in a target measurement range defined by the two-dimensional scanning. A representative background value at each of the measurement points is calculated from the received light and compared with a reference value to provide an alignment rank level indicative of the state of alignment. The state of alignment is determined according to the alignment rank level. A difference value between the representative background values or a standard deviation at the measuring points is calculated and compared with an associated reference value for improving the determination of the state of alignment.

摘要翻译： 一种用于通过用激光束二维地扫描眼睛的前房来测量生物学特性的眼科测量装置。 在由二维扫描限定的目标测量范围内的多个测量点处测量从前房收到的光。 从接收的光计算每个测量点处的代表性背景值，并与参考值进行比较，以提供指示对准状态的对准等级电平。 对齐状态根据对准等级决定。 计算代表性背景值或测量点的标准偏差之间的差值，并将其与相关参考值进行比较，以改善对准状态的确定。

公开(公告)号：US20130309704A1

公开(公告)日：2013-11-21

申请号：US13981893

申请日：2012-01-26

申请人： Katsuya Inada ,  Shigeatsu Endo ,  Taisuke Hirono ,  Takaharu Asano

发明人： Katsuya Inada ,  Shigeatsu Endo ,  Taisuke Hirono ,  Takaharu Asano

IPC分类号： G01N21/82

CPC分类号： G01N21/82 ,  G01N33/54313 ,  G01N33/54393 ,  G01N33/579

摘要： The purpose of the present invention is to improve the measurement accuracy in the measurement of the concentration of a physiologically active biological substance in a sample by a stirring turbidimetry, a light scattering method or an AL-bound beads method, wherein the purpose can be achieved by preventing the occurrence of aggregation or gelation that is caused by the stirring of a mixed solution and is not associated with the physiologically active substance. AL is mixed with a sample containing a physiologically active biological substance, and the aggregation of a protein which is associated with the reaction between AL and the physiologically active substance in the mixed solution is detected while stirring the mixed solution, wherein the occurrence of the aggregation or gelation of a protein which is not associated with the reaction between AL and the physiologically active substance in the mixed solution can be prevented by adding a specific protein that has been heated in advance and/or a specific surfactant to the mixed solution.

摘要翻译： 本发明的目的是通过搅拌比浊法，光散射法或AL结合珠法提高测定生物活性生物物质浓度的测量精度，其目的是实现 通过防止混合溶液的搅拌引起的聚集或凝胶化的发生，与生理活性物质不相关。 将AL与含有生理活性生物物质的样品混合，并在搅拌混合溶液的同时检测与混合溶液中AL与生理活性物质之间的反应相关的蛋白质的聚集，其中发生聚集 或者与混合溶液中的AL与生理活性物质的反应无关的蛋白质的凝胶化可以通过将预先加热的特定蛋白质和/或特定的表面活性剂加入到混合溶液中来防止。