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公开(公告)号：US08835177B2
公开(公告)日：2014-09-16
申请号：US11917853
申请日：2006-06-12
申请人： Takahiro Marui , Tatsuji Enoki , Hiroaki Sagawa , Ikunoshin Kato
发明人： Takahiro Marui , Tatsuji Enoki , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12N15/87 , C07K14/78 , C12N15/86 , C12N15/867
CPC分类号： C12N15/86 , C07K14/78 , C12N2533/52 , C12N2740/13045 , C12N2810/857
摘要： A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.
摘要翻译： 一种将基因转移到脂肪细胞或祖细胞脂肪细胞中的方法，包括在具有逆转录病毒结合位点和靶标的物质存在的情况下用具有外来基因的逆转录病毒载体感染所述脂肪细胞或祖细胞的步骤 分子中的细胞结合位点或具有逆转录病毒结合位点的物质与具有靶细胞结合位点的物质的混合物，所述靶细胞结合位点具有能够结合VLA-5的区域和/或 可以结合VLA-4的区域。
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公开(公告)号：US20100150886A1
公开(公告)日：2010-06-17
申请号：US11990443
申请日：2006-08-10
申请人： Takahiro Marui , Kinuko Magamine , Nobuke Muraki , Akiko Kato , Tatsuji Enoki , Hiroaki Sagawa , Ikunoshin Kato
发明人： Takahiro Marui , Kinuko Magamine , Nobuke Muraki , Akiko Kato , Tatsuji Enoki , Hiroaki Sagawa , Ikunoshin Kato
CPC分类号： A61K35/17 , A61K35/26 , A61K2039/5154 , C07K14/78 , C12N5/0636 , C12N2501/51 , C12N2501/515 , C12N2501/58 , C12N2531/00 , C12N2533/52 , G01N33/547
摘要： A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.
摘要翻译： 一种制备淋巴细胞的方法，其特征在于该方法包括在（a）纤连蛋白，其片段或其混合物，（b）CD3配体和（c）CD28配体存在下进行扩增的步骤。
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公开(公告)号：US09238797B2
公开(公告)日：2016-01-19
申请号：US13003262
申请日：2009-07-07
申请人： Tatsuji Enoki , Fumiko Iwamoto , Toshikazu Nishie , Takahiro Marui , Fuyuko Takashima , Ikunoshin Kato
发明人： Tatsuji Enoki , Fumiko Iwamoto , Toshikazu Nishie , Takahiro Marui , Fuyuko Takashima , Ikunoshin Kato
IPC分类号： C12N5/074
CPC分类号： C12N5/0696 , C12N2501/405 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/608 , C12N2501/727 , C12N2510/00 , C12N2533/52 , C12N2740/15043 , C12N2740/15071
摘要： The present invention relates to a method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors under nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle. The present invention allows induction and growth of pluripotent stem cells at high frequency, and it also allows production of pluripotent stem cells with high efficiency. The nuclear reprogramming factors to be used may be any selected from the group consisting of OCT4, SOX2, c-MYC, KLF4, NANOG and LIN28.
摘要翻译： 本发明涉及含有多能干细胞的细胞群的生产方法，所述方法包括处理在营养缺乏条件下与核重编程因子接触的体细胞的步骤，和/或处理步骤 具有能够阻止细胞周期的药剂的体细胞。 本发明允许高倍数的多能干细胞的诱导和生长，并且还允许以高效率生产多能干细胞。 要使用的核重编程因子可以选自OCT4，SOX2，c-MYC，KLF4，NANOG和LIN28中的任何一种。
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公开(公告)号：US20110117653A1
公开(公告)日：2011-05-19
申请号：US13003262
申请日：2009-07-07
申请人： Tatsuji Enoki , Fumiko Iwamoto , Toshikazu Nishie , Takahiro Marui , Fuyuko Takashima , Ikunoshin K
发明人： Tatsuji Enoki , Fumiko Iwamoto , Toshikazu Nishie , Takahiro Marui , Fuyuko Takashima , Ikunoshin K
IPC分类号： C12N15/86
CPC分类号： C12N5/0696 , C12N2501/405 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/608 , C12N2501/727 , C12N2510/00 , C12N2533/52 , C12N2740/15043 , C12N2740/15071
摘要： The present invention relates to a method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors under nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle. The present invention allows induction and growth of pluripotent stem cells at high frequency, and it also allows production of pluripotent stem cells with high efficiency. The nuclear reprogramming factors to be used may be any selected from the group consisting of OCT4, SOX2, c-MYC, KLF4, NANOG and LIN28.
摘要翻译： 本发明涉及含有多能干细胞的细胞群的生产方法，所述方法包括处理在营养缺乏条件下与核重编程因子接触的体细胞的步骤，和/或处理步骤 具有能够阻止细胞周期的药剂的体细胞。 本发明允许高倍数的多能干细胞的诱导和生长，并且还允许以高效率生产多能干细胞。 要使用的核重编程因子可以选自OCT4，SOX2，c-MYC，KLF4，NANOG和LIN28中的任何一种。
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公开(公告)号：US20100255578A1
公开(公告)日：2010-10-07
申请号：US12303911
申请日：2007-06-07
申请人： Nobuko Muraki , Mitsuko Ideno , Kinuko Nagamine , Fuyuko Takashima , Eiji Kobayashi , Akiko Kato , Takahiro Marui , Hiroaki Sagawa , Tatsuji Enoki , Ikunoshin Kato
发明人： Nobuko Muraki , Mitsuko Ideno , Kinuko Nagamine , Fuyuko Takashima , Eiji Kobayashi , Akiko Kato , Takahiro Marui , Hiroaki Sagawa , Tatsuji Enoki , Ikunoshin Kato
CPC分类号： C07K14/78 , A61K35/17 , A61K2039/5158 , C12N5/0636 , C12N2501/58 , C12N2533/52
摘要： A method of producing lymphocytes characterized by comprising the step of culturing lymphocytes in the presence of a modified recombinant fibronectin fragment which has overlapping parts of the heparin-binding domain of fibronectin. This method makes it possible to achieve a high cell proliferation rate. The lymphocytes obtained thereby are appropriately usable in, for example, adoptive immunotherapy and, therefore, expected as highly useful in the clinical field. Moreover, a novel modified recombinant fibronectin fragment is provided.
摘要翻译： 一种产生淋巴细胞的方法，其特征在于包括在纤连蛋白的肝素结合域的重叠部分的修饰重组纤连蛋白片段存在下培养淋巴细胞的步骤。 该方法可以实现高细胞增殖率。 由此获得的淋巴细胞可适用于例如过继免疫治疗，因此预期在临床领域中非常有用。 此外，提供了新的修饰的重组纤连蛋白片段。
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公开(公告)号：US20090162936A1
公开(公告)日：2009-06-25
申请号：US11917853
申请日：2006-06-12
申请人： Takahiro Marui , Tatsuji Enoki , Hiroaki Sagawa , Ikunoshin Kato
发明人： Takahiro Marui , Tatsuji Enoki , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12N15/867
CPC分类号： C12N15/86 , C07K14/78 , C12N2533/52 , C12N2740/13045 , C12N2810/857
摘要： A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.
摘要翻译： 一种将基因转移到脂肪细胞或祖细胞脂肪细胞中的方法，包括在具有逆转录病毒结合位点和靶标的物质存在的情况下用具有外来基因的逆转录病毒载体感染所述脂肪细胞或祖细胞的步骤 分子中的细胞结合位点或具有逆转录病毒结合位点的物质与具有靶细胞结合位点的物质的混合物，所述靶细胞结合位点具有能够结合VLA-5的区域和/或 可以结合VLA-4的区域。
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公开(公告)号：US08765469B2
公开(公告)日：2014-07-01
申请号：US11990443
申请日：2006-08-10
申请人： Takahiro Marui , Kinuko Nagamine , Nobuko Muraki , Akiko Kato , Tatsuji Enoki , Hiroaki Sagawa , Ikunoshin Kato
发明人： Takahiro Marui , Kinuko Nagamine , Nobuko Muraki , Akiko Kato , Tatsuji Enoki , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12N5/00 , G01N33/547 , A61K35/14
CPC分类号： A61K35/17 , A61K35/26 , A61K2039/5154 , C07K14/78 , C12N5/0636 , C12N2501/51 , C12N2501/515 , C12N2501/58 , C12N2531/00 , C12N2533/52 , G01N33/547
摘要： A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.
摘要翻译： 一种制备淋巴细胞的方法，其特征在于该方法包括在（a）纤连蛋白，其片段或其混合物，（b）CD3配体和（c）CD28配体存在下进行扩增的步骤。
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公开(公告)号：US08216837B2
公开(公告)日：2012-07-10
申请号：US12303911
申请日：2007-06-07
申请人： Nobuko Muraki , Mitsuko Ideno , Kinuko Nagamine , Fuyuko Takashima , Eiji Kobayashi , Akiko Kato , Takahiro Marui , Hiroaki Sagawa , Tatsuji Enoki , Ikunoshin Kato
发明人： Nobuko Muraki , Mitsuko Ideno , Kinuko Nagamine , Fuyuko Takashima , Eiji Kobayashi , Akiko Kato , Takahiro Marui , Hiroaki Sagawa , Tatsuji Enoki , Ikunoshin Kato
IPC分类号： C12N5/071
CPC分类号： C07K14/78 , A61K35/17 , A61K2039/5158 , C12N5/0636 , C12N2501/58 , C12N2533/52
摘要： A method of producing lymphocytes characterized by comprising the step of culturing lymphocytes in the presence of a modified recombinant fibronectin fragment which has overlapping parts of the heparin-binding domain of fibronectin. This method makes it possible to achieve a high cell proliferation rate. The lymphocytes obtained thereby are appropriately usable in, for example, adoptive immunotherapy and, therefore, expected as highly useful in the clinical field. Moreover, a novel modified recombinant fibronectin fragment is provided.
摘要翻译： 一种产生淋巴细胞的方法，其特征在于包括在纤连蛋白的肝素结合域的重叠部分的修饰重组纤连蛋白片段存在下培养淋巴细胞的步骤。 该方法可以实现高细胞增殖率。 由此获得的淋巴细胞可适用于例如过继免疫治疗，因此预期在临床领域中非常有用。 此外，提供了新的修饰的重组纤连蛋白片段。
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