公开(公告)号：US12117444B2

公开(公告)日：2024-10-15

申请号：US16646892

申请日：2019-08-30

申请人： ASAHI KASEI KABUSHIKI KAISHA

发明人： Kotaro Oda ,  Katsuyoshi Takahashi ,  Mika Sugata ,  I-Nung Huang ,  Kazuhiko Arakawa ,  Yuichiro Takahashi

IPC分类号： G01N33/553 ,  C07C229/12 ,  C12N1/06 ,  G01N33/543 ,  G01N33/547 ,  G01N33/558 ,  G01N33/58

CPC分类号： G01N33/54391 ,  C07C229/12 ,  C12N1/06 ,  G01N33/54388 ,  G01N33/547 ,  G01N33/553 ,  G01N33/58 ,  G01N2458/00

摘要： Detection rapidly and with high detection intensity is accomplished while blackening is inhibited during silver enhancement to detect an analyte in a specimen. One embodiment of the present invention provides an enhancing agent to be used for silver enhancement in detection of an analyte in a specimen by metal labeling and silver enhancement, the enhancing agent including:



(a) a silver-containing compound,
(b) a silver ion-reducing agent, and
(c) a reaction rate controller,

wherein the reaction rate controller (c) is a compound selected from the group consisting of compounds represented by the following formula (I):









(wherein:



R1, R2 and R3 each independently represent a hydrogen atom or an optionally substituted monovalent aliphatic hydrocarbon group of 1 to 30 carbon atoms, with the proviso that R1, R2 and R3 are not all hydrogen atoms, or R1 and R2 form a 5-membered ring or 6-membered ring and R3 represents a hydrogen atom or an optionally substituted monovalent aliphatic hydrocarbon group of 1 to 30 carbon atoms;
X represents a divalent hydrocarbon group of 1 to 3 carbon atoms; and
n is an integer of 1 to 3).

公开(公告)号：US20180203003A1

公开(公告)日：2018-07-19

申请号：US15745380

申请日：2016-07-15

申请人： ORPHIDIA LIMITED

发明人： Aron RACHAMIM ,  Ron OREN

IPC分类号： G01N33/545 ,  C07C243/28 ,  G01N33/552 ,  G01N33/553

CPC分类号： G01N33/545 ,  C07C243/28 ,  G01N33/547 ,  G01N33/552 ,  G01N33/553

摘要： A linker molecule and method for treating a substrate surface is provided, which includes a linker molecule with a plurality of moieties capable of resisting non-specific binding of proteins whilst permitting specific binding of a target biomolecule or a biomolecule of interest, including antibodies.

公开(公告)号：US09284393B2

公开(公告)日：2016-03-15

申请号：US14409912

申请日：2013-06-24

申请人： KYUSHU UNIVERSITY ,  NISSAN CHEMICAL INDUSTRIES, LTD.

发明人： Keiji Tanaka ,  Tomoyasu Hirai ,  Kazutaka Shimafuji ,  Masayuki Haraguchi ,  Motonobu Matsuyama

IPC分类号： C08F122/10 ,  G01N33/50 ,  B05D1/00 ,  B05D3/00 ,  C09D135/02 ,  C08F2/00 ,  G01N33/547

CPC分类号： C08F122/105 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B05D1/005 ,  B05D3/007 ,  C09D135/02 ,  G01N33/547

摘要： A biomolecule-compatible highly branched polymer obtained by polymerizing a monomer having an alkylene oxide and two or more radically polymerizable double bonds in a molecule in presence of a polymerization initiator in 5 mol % or more and 200 mol % or less relative to the moles of monomer, wherein molecular terminals of biomolecule-compatible highly branched polymer have one biomolecular site of at least one pair from the group of combination pairs of biotin and avidin, an antigen and antibody, polynucleotide and polynucleotide having complementary base sequence thereof, cDNA and mRNA, enzyme and substrate, an enzyme and product, an enzyme and competitive inhibitor, an enzyme (binding site) and coenzyme, an enzyme (binding site) and triazine dye, protease and protease inhibitor, Fc site and protein A, Fc site and protein G, lectin and sugar, hormone receptor and hormone, DNA and DNA binding protein, heparin and fibronectin, and heparin and laminin.

摘要翻译： 通过在聚合引发剂的存在下，在5摩尔％以上且200摩尔％以下的分子内聚合具有环氧烷烃和2个以上自由基聚合性双键的单体而获得的生物分子相容性高支化聚合物，相对于 单体，其中生物分子相容的高度支化聚合物的分子末端具有与生物素和抗生物素蛋白的组合对中的至少一对的一个生物分子位点，具有其互补碱基序列的抗原和抗体，多核苷酸和多核苷酸，cDNA和mRNA， 酶和底物，酶和产物，酶和竞争性抑制剂，酶（结合位点）和辅酶，酶（结合位点）和三嗪染料，蛋白酶和蛋白酶抑制剂，Fc位点和蛋白A，Fc位点和蛋白G ，凝集素和糖，激素受体和激素，DNA和DNA结合蛋白，肝素和纤连蛋白，以及肝素和层粘连蛋白。

公开(公告)号：US20150299349A1

公开(公告)日：2015-10-22

申请号：US14409912

申请日：2013-06-24

申请人： KYUSHU UNIVERSITY ,  NISSAN CHEMICAL INDUSTRIES, LTD.

发明人： Keiji Tanaka ,  Tomoyasu Hirai ,  Kazutaka Shimafuji ,  Masayuki Haraguchi ,  Motonobu Matsuyama

IPC分类号： C08F122/10 ,  B05D1/00 ,  B05D3/00 ,  C09D135/02

CPC分类号： C08F122/105 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B05D1/005 ,  B05D3/007 ,  C09D135/02 ,  G01N33/547

摘要： A biomolecule-compatible highly branched polymer obtained by polymerizing a monomer having an alkylene oxide and two or more radically polymerizable double bonds in a molecule in presence of a polymerization initiator in 5 mol % or more and 200 mol % or less relative to the moles of monomer, wherein molecular terminals of biomolecule-compatible highly branched polymer have one biomolecular site of at least one pair from the group of combination pairs of biotin and avidin, an antigen and antibody, polynucleotide and polynucleotide having complementary base sequence thereof, cDNA and mRNA, enzyme and substrate, an enzyme and product, an enzyme and competitive inhibitor, an enzyme (binding site) and coenzyme, an enzyme (binding site) and triazine dye, protease and protease inhibitor, Fc site and protein A, Fc site and protein G, lectin and sugar, hormone receptor and hormone, DNA and DNA binding protein, heparin and fibronectin, and heparin and laminin.

摘要翻译： 通过在聚合引发剂的存在下，在5摩尔％以上且200摩尔％以下的分子内聚合具有环氧烷烃和2个以上自由基聚合性双键的单体而获得的生物分子相容性高支化聚合物，相对于 单体，其中生物分子相容的高度支化聚合物的分子末端具有与生物素和抗生物素蛋白的组合对中的至少一对的一个生物分子位点，具有其互补碱基序列的抗原和抗体，多核苷酸和多核苷酸，cDNA和mRNA， 酶和底物，酶和产物，酶和竞争性抑制剂，酶（结合位点）和辅酶，酶（结合位点）和三嗪染料，蛋白酶和蛋白酶抑制剂，Fc位点和蛋白A，Fc位点和蛋白G ，凝集素和糖，激素受体和激素，DNA和DNA结合蛋白，肝素和纤连蛋白，以及肝素和层粘连蛋白。

公开(公告)号：US12111311B2

公开(公告)日：2024-10-08

申请号：US16986662

申请日：2020-08-06

申请人： CANON KABUSHIKI KAISHA ,  CANON MEDICAL SYSTEMS CORPORATION

发明人： Sakae Suda ,  Masao Suzuki ,  Kazumichi Nakahama ,  Keiichiro Tsubaki ,  Kengo Kanazaki ,  Ryo Natori ,  Fumio Yamauchi

IPC分类号： G01N33/543 ,  C08F20/26 ,  C08F212/08 ,  C08F220/32 ,  C08F220/34 ,  C08F220/38 ,  C08L33/06 ,  G01N33/547

CPC分类号： G01N33/54353 ,  C08F212/08 ,  C08F220/346 ,  C08F220/382 ,  G01N33/54313 ,  G01N33/547 ,  C08F20/26 ,  C08F220/325 ,  C08L33/068

摘要： The present disclosure provides a particle showing a small non-specific adsorption, having a reactive functional group for chemically bonding a ligand thereto, and being suitable for an agglutination method; a particle for the agglutination method having a ligand chemically bonded to the particle; a reagent, a kit and a method for detecting a target substance, each for in vitro diagnosis, each of which contains the particle. The particle includes a polymer having a unit having a linker A in a side chain, wherein when both terminals of the linker A are represented by X1 and X2, the linker A has a reactive functional group for chemically bonding a ligand thereto in one of X1 and X2; one of X1 and X2 includes an ester structure; and a sum of bonds between atoms linearly connecting X1 and X2 is 18 to 24 and the X1 and X2 contain CH2 or CH.

公开(公告)号：US20240302360A1

公开(公告)日：2024-09-12

申请号：US18667900

申请日：2024-05-17

申请人： BIOAFFINITY TECHNOLOGIES, INC.

发明人： William E. Bauta

IPC分类号： G01N33/533 ,  G01N33/547

CPC分类号： G01N33/533 ,  G01N33/547

摘要： A compensation bead comprising an amine functionalized bead modified covalently or non-covalently with a carboxyaryl porphyrin, a method for making the amine functionalized bead with the carboxyaryl porphyrin and a method of using the amine functionalized bead with the carboxyaryl porphyrin as a marker for the carboxyaryl porphyrin labeled cells and particles.

公开(公告)号：US20170292947A1

公开(公告)日：2017-10-12

申请号：US15509684

申请日：2015-09-07

申请人： JSR CORPORATION ,  JSR Life Sciences Corporation

发明人： Yuuichi UEYA

IPC分类号： G01N33/547 ,  C08F293/00 ,  G01N33/543 ,  C08F257/02 ,  C08L43/02

CPC分类号： G01N33/547 ,  C08F257/02 ,  C08F293/00 ,  C08F293/005 ,  C08F2438/01 ,  C08L43/02 ,  G01N33/54326 ,  G01N33/54393

摘要： Provided is a solid phase carrier which has high water dispersibility, allows facilitated binding of a ligand to a reactive functional group, and exhibits suppressed non-specific adsorption, and with which, in the case of using the solid phase carrier by having a ligand bound thereto, for example, detection of a target substance can be carried out with high sensitivity and low noise. Disclosed is a solid phase carrier having bound thereto a polymer including a structural unit represented by the following Formula (1) and a structural unit represented by the following Formula (2): wherein in Formula (1), R1 represents a hydrogen atom or a methyl group; and R2 represents an organic group having a zwitterionic structure, in Formula (2), R3 represents a hydrogen atom or a methyl group R4 represents —(C═O)—O—*, —(C═O)—NR6—* (wherein R6 represents a hydrogen atom or a methyl group; and the symbol * represents a position of bonding to R5 in Formula (2)), or a phenylene group; in a case in which R4 represents —(C═O)—O—*, R5 represents a hydrogen atom, or an organic group having a reactive functional group, and in a case in which R4 represents —(C═O)—NR6—* or a phenylene group, R5 represents an organic group having a reactive functional group, provided that R5 is not an organic group having a zwitterionic structure.

公开(公告)号：US20130261023A1

公开(公告)日：2013-10-03

申请号：US13648667

申请日：2012-10-10

申请人： David W. Barnes ,  Angela N. Koehler ,  James Elliot Bradner ,  Ralph Mazitschek ,  Stuart L. Schreiber

发明人： David W. Barnes ,  Angela N. Koehler ,  James Elliot Bradner ,  Ralph Mazitschek ,  Stuart L. Schreiber

IPC分类号： G01N33/68

CPC分类号： G01N33/6803 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/0074 ,  C40B30/04 ,  C40B40/04 ,  C40B80/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/54353 ,  G01N33/547 ,  G01N33/552 ,  G01N33/553

摘要： The present invention provides compositions and methods to facilitate the identification of compounds that are capable of interacting with a biological macromolecule of interest. In one aspect, a composition is provided that comprises an array of one or more types of chemical compounds attached to a solid support using isocyanate or isothiocyanate chemistry, wherein the density of the array of compounds is at least 1000 spots per cm2. In general, these inventive arrays are generated by: (1) providing a solid support, wherein said solid support is functionalized with an isocyanate or isothiocyanate moiety capable of interacting with a desired chemical compound to form a covalent attachment; (2) providing one or more solutions of one or more types of compounds to be attached to the solid support; (3) delivering said one or more types of compounds to the solid support; and (4) catalyzing the attachment of the compound to the solid support, whereby an array is formed and the array of compounds has a density of at least 1000 spots per cm2. In another aspect, the present invention provides methods for utilizing these arrays to identify small molecule partners for biological macromolecules of interest.

公开(公告)号：US12055513B2

公开(公告)日：2024-08-06

申请号：US17773045

申请日：2020-12-29

申请人： LANSION BIOTECHNOLOGY CO., LTD.

发明人： Xingshang Xu ,  Jeffery Chen

IPC分类号： G01N27/327 ,  B01L3/00 ,  G01N33/543 ,  G01N33/547

CPC分类号： G01N27/3276 ,  B01L3/502707 ,  G01N27/3275 ,  G01N27/3277 ,  G01N27/3278 ,  G01N33/5438 ,  G01N33/547 ,  B01L2300/0645

摘要： Provided is a preparation method for an immunoelectrode. The immunoelectrode comprises a substrate, a gold layer, a conductive polymer layer and an antibody layer. The substrate, the gold layer, the conductive polymer layer and the antibody layer are sequentially attached from bottom to top. The preparation method for the immunoelectrode specifically comprises the following steps: (1) preparing the conductive polymer layer: preparing a polypyrrole layer on a gold-plated substrate to obtain a polypyrrole/gold-plated substrate; (2) preparing the immunoelectrode: preparing the antibody layer on the polypyrrole layer to obtain an antibody/polypyrrole/gold-plated substrate; and (3) forming an immunoelectrode system: fixing a bare gold-plated substrate to the outer side of the antibody/polypyrrole/gold-plated substrate to obtain the immunoelectrode system. A polypyrrole material is used for fixing an antibody of a biological recognition element and immobilizing the antibody on the immunoelectrode.

公开(公告)号：US20230258655A1

公开(公告)日：2023-08-17

申请号：US18027624

申请日：2021-06-10

申请人： HANGZHOU ZHEDA DIXUN BIOLOGICAL GENE ENGINEERING CO., LTD.

发明人： Shandong WU ,  Huahao SHEN ,  Yi LIU ,  Zhoujie WU ,  Suling XU ,  Wei LEI ,  Yifei WANG ,  Puyang XU ,  Xiandong ZHOU ,  Weiyue CAI ,  Mingzhi ZHU ,  Xukai YANG

IPC分类号： G01N33/68 ,  G01N33/543 ,  G01N33/547

CPC分类号： G01N33/6854 ,  G01N33/54306 ,  G01N33/547 ,  G01N2333/43582 ,  G01N2800/24

摘要： The present disclosure relates to a kit for detecting dust mite component-specific antibodies, and belongs to the technical field of antibody detection kits. The kit comprises a biotin-polystreptavidin-biotin-dust mite antigen-coated nitrocellulose (NC) membrane, a washing solution, an alkaline phosphatase (ALP)-labeled secondary antibody solution for dust mite component-specific antibodies, and a substrate solution. The kit may be used in cooperation with a fully automated instrument.