公开(公告)号：US07229801B2

公开(公告)日：2007-06-12

申请号：US10475943

申请日：2002-05-27

申请人： Kazutoshi Fujii ,  Yoshinobu Terada ,  Michiyo Yanase ,  Koji Odan ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki ,  Shigetaka Okada

发明人： Kazutoshi Fujii ,  Yoshinobu Terada ,  Michiyo Yanase ,  Koji Odan ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki ,  Shigetaka Okada

IPC分类号： C12P19/60 ,  C12N9/10

CPC分类号： C08B37/0009 ,  C08B30/20 ,  C08B33/00 ,  C12P19/18

摘要： A first method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The maximum value of the sucrose-phosphate ratio of the reaction solution from the start of the reaction to the end of the reaction is no more than about 17. A second method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The reaction is conducted at a temperature of about 40 C to about 70 C.

摘要翻译： 制备葡聚糖的第一种方法包括使含有蔗糖，底漆，无机磷酸盐或葡萄糖-1-磷酸，蔗糖磷酸化酶和葡聚糖磷酸化酶的反应溶液反应生成葡聚糖的步骤。 从反应开始到反应结束的反应溶液的蔗糖磷酸酯比例的最大值不超过约17。第二种葡聚糖的制备方法包括以下步骤：使含有蔗糖的反应溶液， 底物，无机磷酸盐或葡萄糖-1-磷酸盐，蔗糖磷酸化酶和葡聚糖磷酸化酶反应产生葡聚糖。 反应在约40℃至约70℃的温度下进行。

公开(公告)号：US06248566B1

公开(公告)日：2001-06-19

申请号：US08528026

申请日：1995-09-13

申请人： Tadayuki Imanaka ,  Yoshinobu Terada ,  Takeshi Takaha ,  Michiyo Yanase ,  Shigetaka Okada ,  Hiroki Takata ,  Hiroyasu Nakamura ,  Kazutoshi Fujii

发明人： Tadayuki Imanaka ,  Yoshinobu Terada ,  Takeshi Takaha ,  Michiyo Yanase ,  Shigetaka Okada ,  Hiroki Takata ,  Hiroyasu Nakamura ,  Kazutoshi Fujii

IPC分类号： C12P1900

CPC分类号： C08B37/0009 ,  C08B37/0015 ,  C09J105/00 ,  C12P19/14 ,  C12P19/16 ,  C12P19/18

摘要： Glucan with a degree of polymerization of 50 or more includes an inner branched cyclic structure portion and an outer branched structure portion, and methods for producing the same.

摘要翻译： 聚合度为50以上的葡聚糖包括内分支环结构部分和外分支结构部分及其制造方法。

公开(公告)号：US5686132A

公开(公告)日：1997-11-11

申请号：US415152

申请日：1995-03-31

申请人： Takeshi Takaha ,  Michiyo Yanase ,  Shigetaka Okada ,  Hiroki Takata ,  Hiroyasu Nakamura ,  Kazutoshi Fujii

发明人： Takeshi Takaha ,  Michiyo Yanase ,  Shigetaka Okada ,  Hiroki Takata ,  Hiroyasu Nakamura ,  Kazutoshi Fujii

IPC分类号： C08B37/00 ,  C12P19/14 ,  A23G3/00 ,  A61K31/70 ,  C07H1/00

CPC分类号： C12P19/14 ,  C08B37/0009

摘要： The present invention provides glucans and derivatives thereof which are useful as raw materials in the starch processing industries, food and drink compositions, food additive compositions, and starch substitutes for biodegradable plastics, and processes for preparing the same. Particularly, it provides glucans and derivatives thereof having excellent properties such as higher solubility in water than conventional starches, lower viscosity and the glucans are not subject to retrogradation which is observed in conventional starches, and which are capable of preventing the retrogradation of starches.

摘要翻译： 本发明提供了可用作淀粉加工工业中的原料的葡聚糖及其衍生物，食品和饮料组合物，食品添加剂组合物和用于可生物降解塑料的淀粉替代物，以及其制备方法。 特别地，它提供具有优良性能的葡聚糖及其衍生物，例如比常规淀粉更高的在水中的溶解度，较低的粘度和葡聚糖不会在常规淀粉中观察到退化，并且能够防止淀粉的退化。

公开(公告)号：US5827697A

公开(公告)日：1998-10-27

申请号：US560739

申请日：1995-11-20

申请人： Takeshi Takaha ,  Michiyo Yanase ,  Shigetaka Okada ,  Hiroki Takata ,  Hiroyasu Nakamura ,  Kazutoshi Fujii

发明人： Takeshi Takaha ,  Michiyo Yanase ,  Shigetaka Okada ,  Hiroki Takata ,  Hiroyasu Nakamura ,  Kazutoshi Fujii

IPC分类号： C08B37/00 ,  C09J105/00 ,  C12P19/14 ,  C12P19/16 ,  C12P19/18 ,  C12P19/04

CPC分类号： C12P19/16 ,  C08B37/0009 ,  C08B37/0015 ,  C09J105/00 ,  C12P19/14 ,  C12P19/18

摘要： The present invention provides glucans and derivatives thereof which are useful as raw materials in the starch processing industries, food and drink compositions, food additive compositions, and starch substitutes for biodegradable plastics, and processes for preparing the same. Particularly, it provides glucans and derivatives thereof having excellent properties such as higher solubility in water than conventional starches, lower viscosity and the glucans are not subject to retrogradation which is observed in conventional starches, and which are capable of preventing the retrogradation of starches.

摘要翻译： 本发明提供了可用作淀粉加工工业中的原料的葡聚糖及其衍生物，食品和饮料组合物，食品添加剂组合物和用于可生物降解塑料的淀粉替代物，以及其制备方法。 特别地，它提供具有优良性能的葡聚糖及其衍生物，例如比常规淀粉更高的在水中的溶解度，较低的粘度和葡聚糖不会在常规淀粉中观察到退化，并且能够防止淀粉的退化。

公开(公告)号：US07968309B2

公开(公告)日：2011-06-28

申请号：US10566224

申请日：2004-08-31

申请人： Kazutoshi Fujii ,  Masae Iiboshi ,  Michiyo Yanase ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

发明人： Kazutoshi Fujii ,  Masae Iiboshi ,  Michiyo Yanase ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

IPC分类号： C12Q1/48 ,  C12N9/12

CPC分类号： C12N9/1051

摘要： A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the SP having improved thermostability at 37° C. before heating.

摘要翻译： 提供了通过改善天然SP获得的具有改进的热稳定性的蔗糖磷酸化酶（SP）和具有改善的热稳定性的制备SP的方法。 具有改善的热稳定性的SP具有与天然蔗糖磷酸化酶不同的氨基酸残基，在选自对应于位置14的位置，对应于位置29的位置和对应于 基序序列1中的位置44; 对应于位置7的位置，对应于位置19的位置，对应于位置23的位置和对应于图案序列2中的位置34的位置; 和与基序序列3中的位置19相对应的位置，并且其中在55℃下加热在20mM Tris缓冲液（pH7.0）中具有改善的热稳定性的SP后，SP的酶活性在37℃下具有改善的热稳定性。 20分钟，在加热前在37℃下具有改善的热稳定性的SP的酶活性的20％或更多。

公开(公告)号：US20100047891A1

公开(公告)日：2010-02-25

申请号：US12465135

申请日：2009-05-13

申请人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

发明人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

IPC分类号： C12N9/10

CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/04

摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.

摘要翻译： 提供通过改性天然α-葡聚糖磷酸化酶获得的具有改进的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有α-葡聚糖磷酸化酶的酶活性 在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性为具有改善的热稳定性在37℃的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。

公开(公告)号：US07569377B2

公开(公告)日：2009-08-04

申请号：US10560491

申请日：2004-06-15

申请人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

发明人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

IPC分类号： C12N9/12 ,  C07H21/04

CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/04

摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.

摘要翻译： 提供了通过改良天然α-葡聚糖磷酸化酶获得的具有改善的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有 在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性是在37℃下具有改善的热稳定性的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。

公开(公告)号：US07723090B2

公开(公告)日：2010-05-25

申请号：US12465135

申请日：2009-05-13

申请人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

发明人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

IPC分类号： C07H21/04 ,  C12N9/12

CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/04

摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.

摘要翻译： 提供通过改性天然α-葡聚糖磷酸化酶获得的具有改进的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有α-葡聚糖磷酸化酶的酶活性 在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性为具有改善的热稳定性在37℃的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。

公开(公告)号：US20080206822A1

公开(公告)日：2008-08-28

申请号：US10566224

申请日：2004-08-31

申请人： Kazutoshi Fujii ,  Masae Iiboshi ,  Michiyo Yanase ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

发明人： Kazutoshi Fujii ,  Masae Iiboshi ,  Michiyo Yanase ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

IPC分类号： C12P19/12 ,  C12N9/10 ,  C07H21/04 ,  C12N5/00 ,  C12N15/00

CPC分类号： C12N9/1051

摘要： A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the SP having improved thermostability at 37° C. before heating.

摘要翻译： 提供了通过改善天然SP获得的具有改进的热稳定性的蔗糖磷酸化酶（SP）和具有改善的热稳定性的制备SP的方法。 具有改善的热稳定性的SP具有与天然蔗糖磷酸化酶不同的氨基酸残基，在选自对应于位置14的位置，对应于位置29的位置和对应于位置的位置的至少一个位置 基序序列1中的位置44; 对应于位置7的位置，对应于位置19的位置，对应于位置23的位置和对应于图案序列2中的位置34的位置; 和与基序序列3中的位置19相对应的位置，并且其中在55℃下加热在20mM Tris缓冲液（pH7.0）中具有改善的热稳定性的SP后，SP的酶活性在37℃下具有改善的热稳定性。 20分钟，在加热前在37℃下具有改善的热稳定性的SP的酶活性的20％或更多。

公开(公告)号：US20060275875A1

公开(公告)日：2006-12-07

申请号：US10560491

申请日：2004-06-15

申请人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

发明人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

IPC分类号： C12P19/04 ,  C07H21/04 ,  C12P21/06 ,  C12N9/12 ,  C12N9/16

CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/04

摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.

摘要翻译： 提供了通过改良天然α-葡聚糖磷酸化酶获得的具有改善的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有 在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性是在37℃下具有改善的热稳定性的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。