利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.023 秒)

    Expression of Genes from Gram Negative Bacteria in Fungi
    1.
    发明申请
    Expression of Genes from Gram Negative Bacteria in Fungi 审中-公开
    革兰氏阴性菌在真菌中的表达

    公开(公告)号：US20100261259A1

    公开(公告)日：2010-10-14

    申请号：US12376372

    申请日：2007-08-03

    申请人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    发明人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    IPC分类号： C12N1/19 ,  C12N1/15 ,  C07H21/04

    CPC分类号： C12N9/16 ,  C07K2319/02 ,  C12N15/80 ,  C12P21/02

    摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.

    摘要翻译： 本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。 在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一 编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与 存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。

    Recombinant Production of Serum Albumin
    4.
    发明申请
    Recombinant Production of Serum Albumin 审中-公开
    重组血清白蛋白的生产

    公开(公告)号：US20090280534A1

    公开(公告)日：2009-11-12

    申请号：US11721813

    申请日：2005-12-22

    申请人： Bjarke Christensen ,  Carsten Hjort ,  Thomas Agersten Poulsen ,  Poul Bach

    发明人： Bjarke Christensen ,  Carsten Hjort ,  Thomas Agersten Poulsen ,  Poul Bach

    IPC分类号： C12P21/02 ,  C12N15/11

    CPC分类号： C07K14/765 ,  A61K9/16 ,  A61K38/38 ,  C12N15/67

    摘要： The present invention relates to modified nucleotide sequences encoding serum albumin, wherein the mRNA sequence has been codon optimized for expression in a filamentous host cell. Furthermore the present invention relates to serum albumin produced in filamentous fungi or loaded by contacting serum albumin with an extract of a filamentous fungi culture. In an-other aspect the present invention relates to the use of said serum albumin in serum free cell culture media and also to a method of drying and agglomerating serum albumin thereby im-proving wettability and dispersability.

    摘要翻译： 本发明涉及编码血清白蛋白的修饰的核苷酸序列，其中mRNA序列已被密码子优化用于在丝状宿主细胞中表达。 此外，本发明涉及在丝状真菌中产生的血清白蛋白或通过使血清白蛋白与丝状真菌培养物的提取物接触来加载。 另一方面，本发明涉及所述血清白蛋白在无血清细胞培养基中的用途，还涉及一种干燥和附聚血清白蛋白从而提高润湿性和分散性的方法。