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    Expression of Genes from Gram Negative Bacteria in Fungi
    3.
    发明申请
    Expression of Genes from Gram Negative Bacteria in Fungi 审中-公开
    革兰氏阴性菌在真菌中的表达

    公开(公告)号：US20100261259A1

    公开(公告)日：2010-10-14

    申请号：US12376372

    申请日：2007-08-03

    申请人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    发明人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    IPC分类号： C12N1/19 ,  C12N1/15 ,  C07H21/04

    CPC分类号： C12N9/16 ,  C07K2319/02 ,  C12N15/80 ,  C12P21/02

    摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.

    摘要翻译： 本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。 在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一 编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与 存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。

    HAFNIA PHYTASE VARIANTS
    4.
    发明申请
    HAFNIA PHYTASE VARIANTS 有权

    公开(公告)号：US20100083392A1

    公开(公告)日：2010-04-01

    申请号：US12568312

    申请日：2009-09-28

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： A01K67/00 ,  C12N9/16 ,  C12P1/00 ,  C12P7/06 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00 ,  C12P21/00 ,  A01H5/00 ,  A23L1/28 ,  A23K1/18 ,  A23K1/16 ,  C12G1/00 ,  C12C11/00 ,  C12C7/00

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Hafnia Phytase Variants
    5.
    发明申请
    Hafnia Phytase Variants 有权
    Hafnia植酸酶变体

    公开(公告)号：US20120225468A1

    公开(公告)日：2012-09-06

    申请号：US13472822

    申请日：2012-05-16

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： C12N9/16

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Hafnia phytase variants
    6.
    发明授权
    Hafnia phytase variants 有权
    Hafnia植酸酶变体

    公开(公告)号：US08206962B2

    公开(公告)日：2012-06-26

    申请号：US12568312

    申请日：2009-09-28

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： C12N9/14 ,  C12Q1/34 ,  C07H21/04

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Hafnia phytase variants
    7.
    发明授权
    Hafnia phytase variants 有权
    Hafnia植酸酶变体

    公开(公告)号：US08507240B2

    公开(公告)日：2013-08-13

    申请号：US13472822

    申请日：2012-05-16

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Lars Kobberoee Skov ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Lars Kobberoee Skov ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Jesper Vind

    IPC分类号： C12N9/16 ,  A23K1/165 ,  A23L1/105 ,  A23L1/20

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。