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    Polypeptides having interferon activity
    1.
    发明授权
    Polypeptides having interferon activity 失效
    具有干扰素活性的多肽

    公开(公告)号：US4921699A

    公开(公告)日：1990-05-01

    申请号：US257900

    申请日：1988-10-14

    申请人： Thomas M. DeChiara ,  Stanley J. Tarnowski, Jr.

    发明人： Thomas M. DeChiara ,  Stanley J. Tarnowski, Jr.

    IPC分类号： A61K38/00 ,  C07K14/555 ,  C07K14/56 ,  C12N15/10 ,  C12N15/20

    CPC分类号： C12N15/10 ,  C07K14/555 ,  C07K14/56 ,  A61K38/00

    摘要： A polypeptide having interferon activity which has an amino acid sequence corresponding to an amino acid sequence of an interferon selected from rIFN-.alpha., hybrid rIFN-.alpha., rIFN-.beta. and rIFN-.gamma. interferons in which at least one cysteine residue in the amino acid sequence has been replaced by an amino acid residue which is incapable of forming intermolecular disulfide bonds is provided. There are also provided a dsDNA sequence encoding the novel polypeptide; a replicable plasmidic expression vehicle containing the dsDNA encoding the novel polypeptides of the invention; a microorganism which has been transformed with the replicable plasmidic expression vehicle; and a method of preparing the dsDNA which encodes the novel polypeptides of the invention.

    摘要翻译： 具有干扰素活性的多肽，其具有对应于选自rIFN-α，杂合rIFN-α，rIFN-β和rIFN-γ干扰素的干扰素的氨基酸序列的氨基酸序列，其中氨基酸中的至少一个半胱氨酸残基 序列被替代为不能形成分子间二硫键的氨基酸残基。 还提供了编码新多肽的dsDNA序列; 含有编码本发明新多肽的dsDNA的可复制质粒表达载体; 用可复制的质粒表达载体转化的微生物; 以及编码本发明的新型多肽的dsDNA的制备方法。

    Polypeptides having interferon activity
    2.
    发明授权
    Polypeptides having interferon activity 失效
    具有干扰素活性的多肽

    公开(公告)号：US4816566A

    公开(公告)日：1989-03-28

    申请号：US827674

    申请日：1986-02-10

    申请人： Thomas M. DeChiara ,  Stanley J. Tarnowski, Jr.

    发明人： Thomas M. DeChiara ,  Stanley J. Tarnowski, Jr.

    IPC分类号： A61K38/00 ,  C07K14/555 ,  C07K14/56 ,  C12N15/10 ,  C07K13/00 ,  A61K45/02 ,  C12P21/00

    CPC分类号： C12N15/10 ,  C07K14/555 ,  C07K14/56 ,  A61K38/00 ,  Y10S435/811 ,  Y10S930/142

    摘要： A polypeptide having interferon activity which has an amino acid sequence corresponding to an amino acid sequence of an interferon selected from rIFN-.alpha., hybrid rIFN-.alpha., rIFN-.beta. and rIFN-.gamma. interferons in which at least one cysteine residue in the amino acid sequence has been replaced by an amino acid residue which is incapable of forming intermolecular disulfide bonds is provided. There are also provided a dsDNA sequence encoding the novel polypeptide; a replicable plasmidic expression vehicle containing the dsDNA encoding the novel polypeptides of the invention; a microorganism which has been transformed with the replicable plasmidic expression vehicle; and a method of preparing the dsDNA which encodes the novel polypeptides of the invention.

    摘要翻译： 具有干扰素活性的多肽，其具有对应于选自rIFN-α，杂合rIFN-α，rIFN-β和rIFN-γ干扰素的干扰素的氨基酸序列的氨基酸序列，其中氨基酸中的至少一个半胱氨酸残基 序列被替代为不能形成分子间二硫键的氨基酸残基。 还提供了编码新多肽的dsDNA序列; 含有编码本发明新多肽的dsDNA的可复制质粒表达载体; 用可复制的质粒表达载体转化的微生物; 以及编码本发明的新型多肽的dsDNA的制备方法。

    Method for targeting transcriptionally active loci
    3.
    发明授权
    Method for targeting transcriptionally active loci 有权
    靶向转录活性位点的方法

    公开(公告)号：US07473557B2

    公开(公告)日：2009-01-06

    申请号：US10957760

    申请日：2004-10-04

    申请人： Aris N. Economides ,  Thomas M. DeChiara ,  George D. Yancopoulos

    发明人： Aris N. Economides ,  Thomas M. DeChiara ,  George D. Yancopoulos

    IPC分类号： C12N15/00 ,  C12N15/87

    CPC分类号： C12N15/8509 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0393 ,  C12N15/907 ,  C12N2800/30 ,  C12N2830/00

    摘要： The present invention provides a method of achieving very high targeting efficiency by utilizing targeting vectors that utilize promoter-less selection cassettes and which are engineered to targeted into transcriptionally active loci. In particular, the invention provides a method for targeting promoter-less selection cassettes into transcriptionally active loci in stem cells or other eukaryotic cells with much greater efficiency than previously observed with other methods, thus reducing the number of drug-resistant clones to be screened or eliminating the need to screen for targeted cells altogether. The invention also encompasses the DNA targeting vectors, the targeted cells, as well as non-human organisms, especially mice, created from the targeted cells.

    摘要翻译： 本发明提供了一种通过利用利用无启动子选择盒并被设计成靶向转录活性位点的靶向载体来实现非常高的靶向效率的方法。 特别地，本发明提供了一种将无启动子选择盒定位在干细胞或其他真核细胞中的转录活性基因座的方法，其效率比先前用其它方法观察到的效率高得多，从而减少要筛选的耐药性克隆的数目 无需完全筛选靶细胞。 本发明还包括从靶细胞产生的DNA靶向载体，靶细胞以及非人生物，特别是小鼠。

    Methods of stimulating cartilage formation
    4.
    发明授权
    Methods of stimulating cartilage formation 有权
    刺激软骨形成的方法

    公开(公告)号：US06908744B1

    公开(公告)日：2005-06-21

    申请号：US10221599

    申请日：2001-02-20

    申请人： Thomas M. DeChiara ,  Carrie A. Kimble ,  George D. Yancopoulos

    发明人： Robert Kimble

    IPC分类号： C07K14/705 ,  C12Q1/02 ,  C12Q1/00 ,  G01N33/53

    CPC分类号： C07K14/705

    摘要： The disruption of the murine ROR2 gene leads to profound skeletal abnormalities, with essentially all endochondrally derived bones foreshortened and/or misshapen, albeit to differing degrees. ROR2 is selectively expressed in the chondrocytes of all developing cartilage anlagen, where it plays a critical role during initial growth and patterning, as well as subsequently in the proliferating chondrocytes of mature growth plates, where it is required for normal expansion. As ROR2 appears to play a critical role in cartilage formation and it may be useful in developing therapeutic strategies to treat diseases of cartilage such as osteoarthritis.

    摘要翻译： 鼠ROR2基因的破坏导致严重的骨骼异常，基本上所有的内源性骨骼都被缩短和/或畸形，尽管程度不同。 ROR2在所有发育中的软骨成骨细胞的软骨细胞中选择性表达，其在初始生长和图案化过程中起关键作用，随后在成熟生长板的增殖性软骨细胞中进行正常扩增。 由于ROR2似乎在软骨形成中发挥关键作用，并且可能有助于开发用于治疗软骨疾病如骨关节炎的治疗策略。