公开(公告)号：US20190216063A1

公开(公告)日：2019-07-18

申请号：US16371824

申请日：2019-04-01

申请人： The University Court of the University of Edinburgh

发明人： Christopher Bruce Alexander Whitelaw ,  Christopher James Palgrave ,  Simon Geoffrey Lillico

IPC分类号： A01K67/027 ,  C12N9/22 ,  C07K14/47

CPC分类号： A01K67/0276 ,  A01K67/0275 ,  A01K2217/07 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2227/108 ,  A01K2267/02 ,  A01K2267/03 ,  C07K14/4702 ,  C12N9/22

摘要： The present invention relates to a genetically edited animal, especially to a genetically edited pig in which expression or activity of the RELA protein has been modified. Such pigs have at least partial protection against the African Swine Fever Virus. The invention also provides, a cell nucleus, germ cell, stem cell, gamete, blastocyst, embryo, foetus and/or donor cell of a non-human animal comprising a genetic modification which alters the expression or function of RELA protein, methods for editing the genome of animals and methods for screening the efficacy of a pharmaceutical agent in such an animal.

公开(公告)号：US20180271070A1

公开(公告)日：2018-09-27

申请号：US16001070

申请日：2018-06-06

申请人： Regeneron Pharmaceuticals, Inc.

发明人： John McWhirter ,  Cagan Gurer ,  Lynn Macdonald ,  Andrew J. Murphy

IPC分类号： A01K67/027 ,  G01N33/50 ,  C07K14/47 ,  C07K14/705 ,  C12N15/85

CPC分类号： A01K67/0278 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/07 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0387 ,  C07K14/47 ,  C07K14/70575 ,  C07K2319/00 ,  C12N15/8509 ,  G01N33/5005

摘要： Non-human animals, cells, methods and compositions for making and using the same are provided, wherein the non-human animals and cells comprise a humanized a proliferation-inducing ligand gene. Non-human animals and cells that express a human or humanized a proliferation-inducing ligand protein from an endogenous a proliferation-inducing ligand locus are described.

公开(公告)号：US20170283831A1

公开(公告)日：2017-10-05

申请号：US15620098

申请日：2017-06-12

申请人： The Broad Institute Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Feng Zhang ,  Omar O. Abudayyeh ,  James E. Dahlman ,  Patrick Hsu ,  David A. Scott

IPC分类号： C12N15/90 ,  A01K67/027 ,  C12N15/11 ,  C07K14/47 ,  C12N9/22 ,  C12N9/96

CPC分类号： C12N15/907 ,  A01K67/0275 ,  A01K2217/07 ,  A01K2227/105 ,  C07K14/4702 ,  C12N9/22 ,  C12N9/96 ,  C12N15/11 ,  C12N15/111 ,  C12N2310/20 ,  C12Y301/21004

摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems, wherein the guide sequence is modified by secondary structure to increase the specificity of the CRISPR-Cas system and whereby the secondary structure can protect against exonuclease activity and allow for 5′ additions to the guide sequence.

公开(公告)号：US20170191065A1

公开(公告)日：2017-07-06

申请号：US15307050

申请日：2015-05-04

申请人： Forrest Innovations Ltd.

发明人： Nitzan PALDI ,  Humberto BONCRISTIANI JUNIOR ,  Eyal MAORI ,  Avital WEISS ,  Emerson Soares BERNARDES

IPC分类号： C12N15/113 ,  A01K67/033 ,  A01N25/00 ,  A01N57/16 ,  A01N63/00

CPC分类号： C12N15/1138 ,  A01K67/0339 ,  A01K2217/07 ,  A01K2227/706 ,  A01K2267/02 ,  A01N25/002 ,  A01N25/006 ,  A01N25/02 ,  A01N25/10 ,  A01N57/16 ,  A01N63/00 ,  A01N65/03 ,  C07K14/415 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8245 ,  C12N15/8261 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8281 ,  C12N2310/14 ,  C12N2320/30 ,  C12N2320/32 ,  C12N2320/35

摘要： A method of enhancing resistance of a mosquito to a pathogen is disclosed. The method comprising administering to a mosquito an isolated nucleic acid agent comprising a nucleic acid sequence which specifically downregulates an expression of at least one mosquito or pathogen gene wherein a product of said mosquito or pathogen gene participates in infection and/or growth of the pathogen in the mosquito.

公开(公告)号：US20170071208A1

公开(公告)日：2017-03-16

申请号：US15308394

申请日：2015-05-04

申请人： Forrest Innovations Ltd.

发明人： Nitzan PALDI ,  Humberto Freire BONCRISTIANI JUNIOR ,  Eyal MAORI ,  Avital WEISS ,  Emerson Soares BERNARDES

IPC分类号： A01N57/16 ,  A01N25/10 ,  C12N15/113

CPC分类号： C12N15/1138 ,  A01K67/0339 ,  A01K2217/07 ,  A01K2227/706 ,  A01K2267/02 ,  A01N25/002 ,  A01N25/006 ,  A01N25/02 ,  A01N25/10 ,  A01N57/16 ,  A01N63/00 ,  A01N65/03 ,  C07K14/415 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8245 ,  C12N15/8261 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8281 ,  C12N2310/14 ,  C12N2320/30 ,  C12N2320/32 ,  C12N2320/35

摘要： A composition-of-matter for mosquito control is provided. The composition comprises a cell which comprises an exogenous naked dsRNA which specifically down-regulates expression of a gene being endogenous to a mosquito or which specifically down-regulated expression of a gene being endogenous to a mosquito pathogen. Further provided is a composition-of-matter for mosquito control, comprising a cell comprising a nucleic acid larvicide. Also provided are methods of producing and using the compositions.

摘要翻译： 提供了一种用于灭蚊控制的物质组成。 组合物包含细胞，其包含特异性下调蚊子内源基因表达的外源性裸dsRNA，或特异性下调蚊子病原体内源基因的表达。 还提供了一种用于灭蚊控制的物质组合物，其包括含有核酸杀幼虫剂的细胞。 还提供了生产和使用组合物的方法。

公开(公告)号：US09567573B2

公开(公告)日：2017-02-14

申请号：US13066801

申请日：2011-04-25

申请人： Philip D. Gregory ,  Michael C. Holmes

发明人： Philip D. Gregory ,  Michael C. Holmes

IPC分类号： C12N9/22 ,  A01K67/027 ,  C12N15/10 ,  C12N15/90 ,  A61K38/00

CPC分类号： C12N9/22 ,  A01K67/0275 ,  A01K2207/05 ,  A01K2217/07 ,  A01K2227/105 ,  A01K2267/03 ,  A61K38/00 ,  C07K2319/81 ,  C12N15/102 ,  C12N15/907

摘要： Disclosed herein are methods and compositions for genome editing of a Rosa locus, using fusion proteins comprising a DNA binding domain and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

摘要翻译： 本文公开了使用包含DNA结合结构域和切割结构域或裂解半结构域的融合蛋白的罗莎基因座的基因组编辑的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。

公开(公告)号：US20160029604A1

公开(公告)日：2016-02-04

申请号：US14698561

申请日：2015-04-28

申请人： RECOMBINETICS, INC.

发明人： Scott C. Fahrenkrug ,  Daniel F. Carlson

IPC分类号： A01K67/027 ,  C12N15/90

CPC分类号： A01K67/0276 ,  A01K67/0275 ,  A01K2217/07 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/101 ,  A01K2227/108 ,  A01K2267/02 ,  C12N15/907

摘要： Materials and methods for making multiplex gene edits in cells or embryos are presented. Further methods include animals and methods of making the same. Methods of making chimeric animals are presented, as well as chimeric animals.

摘要翻译： 提出了在细胞或胚胎中进行多重基因编辑的材料和方法。 其他方法包括动物及其制造方法。 呈现嵌合动物的方法以及嵌合动物。

公开(公告)号：US09096870B2

公开(公告)日：2015-08-04

申请号：US14176484

申请日：2014-02-10

申请人： Regeneron Pharmaceuticals, Inc.

发明人： David Frendewey ,  David Jonathan Heslin ,  Ka-Man Venus Lai ,  David M. Valenzuela

IPC分类号： C12N15/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/873 ,  A01K67/027 ,  C12N15/85 ,  C12N15/90

CPC分类号： C12N15/85 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2217/07 ,  A01K2217/075 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/03 ,  C12N5/0606 ,  C12N9/1241 ,  C12N15/8509 ,  C12N15/873 ,  C12N15/907 ,  C12N2800/22 ,  C12N2800/30 ,  C12N2840/102 ,  C12Y207/07

摘要： Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal.

摘要翻译： 提供靶向构建体和使用它们的方法用于细胞和非人动物中核酸序列的分化依赖性修饰。 提供了包含与重组酶可操作地连接的启动子的靶向构建体，其中所述启动子驱动分化细胞中但不是未分化细胞的重组酶的转录。 启动子包括Blimp1，Prm1，Gata6，Gata4，Igf2，Lhx2，Lhx5和Pax3。 可以使用可操作地连接到3'-UTR的重组酶基因来除去具有通过重组酶位点两侧的盒的靶向构建体，其包含在未分化细胞中但不在分化细胞中转录的miRNA的识别位点。 构建体可以包括在靶向载体中，并且可以用于自动修饰或从ES细胞，非人胚胎或非人动物中切除选择盒。

公开(公告)号：US08951789B2

公开(公告)日：2015-02-10

申请号：US12743065

申请日：2008-10-14

申请人： Mitsuo Oshimura ,  Yasuhiro Kazuki ,  Takashi Matsuoka ,  Kazuma Tomizuka ,  Takeshi Oshima

发明人： Mitsuo Oshimura ,  Yasuhiro Kazuki ,  Takashi Matsuoka ,  Kazuma Tomizuka ,  Takeshi Oshima

IPC分类号： C12N15/63 ,  C12N5/06 ,  C12N15/85 ,  A01K67/027 ,  C12N9/02 ,  G01N33/50 ,  A01K67/00

CPC分类号： C12N15/8509 ,  A01K67/0278 ,  A01K2217/00 ,  A01K2217/07 ,  A01K2227/105 ,  C12N9/0071 ,  C12N9/0077 ,  C12N2800/208 ,  G01N33/5088

摘要： This invention relates to a mammalian artificial chromosome vector, which retains a human chromosome 7 fragment comprising human cytochrome P450 genes and is transmittable to progeny, wherein the human chromosome 7 fragment retains a region of approximately 1 Mb±500 Kb in size comprising at least a human CYP3A gene cluster, which region is located between chromosome markers AC004922 and AC073842, and to a non-human mammalian animal retaining the vector.

摘要翻译： 本发明涉及一种哺乳动物人造染色体载体，其保留包含人细胞色素P450基因的人染色体7片段，并可传播给子代，其中人染色体7片段保留大小约1Mb±500Kb的区域，其包含至少一个 人类CYP3A基因簇，该区域位于染色体标记物AC004922和AC073842之间，以及保留载体的非人哺乳动物。

公开(公告)号：US20140344963A1

公开(公告)日：2014-11-20

申请号：US14286468

申请日：2014-05-23

申请人： Board of Regents of The University of Texas System

发明人： Franklin Kent Hamra

IPC分类号： A01K67/027 ,  A01K67/02

CPC分类号： A01K67/0271 ,  A01K67/02 ,  A01K67/0275 ,  A01K2217/07 ,  A01K2227/10 ,  A01K2227/105 ,  C12N5/061 ,  C12N2500/44 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2800/90

摘要： A spermatogonial stem cell line that is derived from testes of rats characterized by a desirable genetic background can serve as a source for cells to transplant into male-sterile recipient animals that are immuno-compatible with the spermatogonial line. Rat cells thus transplanted readily develop into fertilization-competent, haploid male gametes, with little or no endogenous sperm competition generated by the testes of the male-sterile recipient. This approach, constituting the first vector system for the use of rat spermatogonial lines from in vitro culture in generating mutant rats on a desired genetic background, effects maximal germline transmission of donor haplotypes from the transplanted spermatogonial cells.

摘要翻译： 来源于具有所需遗传背景的大鼠睾丸的精原干细胞系可以作为细胞移植到与精原细胞系免疫相容的雄性不育受体动物的来源。 因此移植的大鼠细胞很容易发育成受精能力的单倍体雄性配子，雄性不育接受者的睾丸产生的内源性精子竞争很少或没有。 构建用于在所需遗传背景下产生突变大鼠的来自体外培养的大鼠精原细胞系的第一载体系统影响来自移植的精原细胞的供体单倍型的最大种系传播。