CHROMOGENIC TESTS IN THE PRESENCE OF CELLS
    2.
    发明申请
    CHROMOGENIC TESTS IN THE PRESENCE OF CELLS 审中-公开
    细胞存在的染色体检测

    公开(公告)号:US20070154975A1

    公开(公告)日:2007-07-05

    申请号:US11608943

    申请日:2006-12-11

    IPC分类号: C12Q1/37

    摘要: The present invention concerns a method for the colorimetric measurement of enzyme activities comprising the following steps: preparing a cell suspension or a cell lysate containing cell components adding a chromogenic substrate at the start of the enzyme reaction adding a first additional substance to stop the enzyme reaction colorimetric measurement wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction.

    摘要翻译: 本发明涉及一种酶活性比色测定方法,包括以下步骤:在酶反应开始时,制备含有细胞组分的细胞悬液或含细胞裂解液的细胞成分,加入第一种附加物质以停止酶反应 比色测量,其中在酶反应终止期间或之后添加减少由细胞或细胞成分引起的光散射和/或吸收的第二附加物质。

    Reducing sample turbidity in measurement of enzymatic activity in cell lysates
    4.
    发明授权
    Reducing sample turbidity in measurement of enzymatic activity in cell lysates 失效
    在细胞裂解物中酶活性的测量中减少样品浊度

    公开(公告)号:US07919268B2

    公开(公告)日:2011-04-05

    申请号:US12409038

    申请日:2009-03-23

    IPC分类号: C12Q1/32

    摘要: Disclosed are methods and kits for measuring enzyme activity in which the turbidity of a sample caused by cell components is minimized by particular additives. The methods include the steps of providing a cell suspension or a cell lysate containing cell components, adding a chromogenic substrate at the start of the enzyme reaction, adding a first additional substance to stop the enzyme reaction, and making a colorimetric measurement, wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction. In particular, a method is disclosed wherein the enzyme is lactate dehydrogenase (LDH) and the first additional substance to stop the reaction is HCl.

    摘要翻译: 公开了用于测量酶活性的方法和试剂盒,其中由细胞组分引起的样品的浊度被特定的添加剂最小化。 所述方法包括以下步骤:提供含有细胞成分的细胞悬液或细胞裂解物,在酶反应开始时加入显色底物,加入第一附加物质以停止酶反应,进行比色测定,其中第二 在酶反应终止期间或之后添加减少由细胞或细胞成分引起的光散射和/或吸收的附加物质。 特别地,公开了一种方法,其中酶是乳酸脱氢酶(LDH),停止反应的第一种另外的物质是HCl。

    COLORIMETRIC MEASUREMENT OF ENZYMATIC ACTIVITY IN CELL LYSATES
    5.
    发明申请
    COLORIMETRIC MEASUREMENT OF ENZYMATIC ACTIVITY IN CELL LYSATES 失效
    细胞LYSATE中酶活性的色度测量

    公开(公告)号:US20090181417A1

    公开(公告)日:2009-07-16

    申请号:US12409038

    申请日:2009-03-23

    IPC分类号: C12Q1/32

    摘要: The present invention concerns a method for the calorimetric measurement of enzyme activity comprising the following steps: preparing a cell suspension or a cell lysate containing cell components, adding a chromogenic substrate at the start of the enzyme reaction, adding a first additional substance to stop the enzyme reaction, and making a colorimetric measurement, wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction.

    摘要翻译: 本发明涉及一种用于量热测定酶活性的方法,包括以下步骤:制备含有细胞组分的细胞悬浮液或细胞裂解液,在酶反应开始时加入显色底物,加入第一种附加物质以停止 酶反应和进行比色测量,其中在酶反应终止期间或之后添加减少由细胞或细胞成分引起的光散射和/或吸收的第二附加物质。

    Wind power machine
    6.
    发明申请
    Wind power machine 审中-公开
    风力发电机

    公开(公告)号:US20050155346A1

    公开(公告)日:2005-07-21

    申请号:US10758842

    申请日:2004-01-16

    申请人: Thomas Nikolaus

    发明人: Thomas Nikolaus

    摘要: A wind power machine for production of energy, comprises at least one rotor element (5) which can be driven by the wind and having an output load (15), in particular a generator (16), which is connected directly or indirectly to it, the rotor element (5) is intended to drive one or more hydraulic pumps (7) directly or indirectly.

    摘要翻译: 一种用于生产能量的风力发电机,包括至少一个转子元件(5),该转子元件可被风驱动,并具有直接或间接连接到其的输出负载(15),特别是发电机(16) 转子元件(5)旨在直接或间接地驱动一个或多个液压泵(7)。

    Method and device for introducing a liquid or gaseous treatment medium
into a flue gas flow
    8.
    发明授权
    Method and device for introducing a liquid or gaseous treatment medium into a flue gas flow 失效
    将液体或气态处理介质引入烟气流中的方法和装置

    公开(公告)号:US5676071A

    公开(公告)日:1997-10-14

    申请号:US553489

    申请日:1996-02-27

    摘要: A method and apparatus for introducing a liquid or gaseous treatment medium into a flue gas stream wherein a total adjustment value for controlling the amount of active substance in the treatment medium is generated by an actual value measurement of a flue gas component and a comparison with a set value, and the treatment medium is injected through a plurality of nozzles into the flue gas stream flowing through a flow chamber. The flow chamber is divided into a three-dimensional matrix of partial flow chambers, each of which comprises a nozzle and measuring devices for detecting the temperature and flow speed of the flue gas stream in the partial flow chambers. The active substance is injected with a constant volume flow of treatment medium in an amount determined by calculation of an individual adjustment value of active substance for each nozzle. The total of the individual partial adjustment values does not exceed the total adjustment value of the active substance. Nozzles having a partial adjustment value of zero are not activated such that, in each column of the matrix injection occurs only in a single partial flow chamber.

    摘要翻译: PCT No.PCT / CH95 / 00063 Sec。 371日期1996年2月27日 102(e)日期1996年2月27日PCT 1995年3月21日PCT PCT。 WO95 / 25931 PCT出版物 日期1995年9月28日一种用于将液体或气体处理介质引入烟道气流的方法和装置,其中通过烟道气成分的实际值测量产生用于控制处理介质中活性物质的量的总调节值 并且与设定值进行比较,并且处理介质通过多个喷嘴注入流过流动室的烟道气流中。 流动室被分成三部分流动室的矩阵,其中每个都包括一个喷嘴和用于检测部分流动室中烟气流的温度和流速的测量装置。 以恒定体积流量的处理介质注入活性物质,其量通过计算每个喷嘴的活性物质的单独调整值而确定。 各个部分调整值的总和不超过活性物质的总调整值。 部分调整值为零的喷嘴不被激活,使得在矩阵喷射的每一列只发生在单个部分流动室中。

    Grate element and grate with fluid cooling
    9.
    发明授权
    Grate element and grate with fluid cooling 失效
    炉排元件和炉排与流体冷却

    公开(公告)号:US5899150A

    公开(公告)日:1999-05-04

    申请号:US866432

    申请日:1997-05-30

    摘要: The grate element (1, 2) is made in three pieces in the sample embodiment, and consists of a central main part (10) and two side parts (11, 12) attached thereto. The main part (10) is pierced by parallel straight-line bores, which form the straight-line conduit sections. These straight-line conduit sections (13) are flow-connected with reversers (14) in the respective side parts (11, 12). The inlet (15) for the coolant fluid is located in the rear part of the grate element, whereas the outlet (16) is located in the head area (7) of the grate element. In the outlet (16) there is a temperature probe (17) for monitoring the temperature of the coolant fluid. The grate element head (7) has recesses (18) in its foot (8) which open downward, serving as air escape orifices for the primary air, which is supplied from beneath.

    摘要翻译: 在样品实施例中,格栅元件(1,2)制成三片,由中心主体部分(10)和与其相连的两个侧面部分(11,12)组成。 主要部分(10)由平行的直线孔刺穿,形成直线导管段。 这些直线导管部分(13)在相应的侧部部分(11,12)中与反向器(14)流动连接。 用于冷却剂流体的入口(15)位于炉排元件的后部,而出口(16)位于炉排元件的头部区域(7)中。 在出口(16)中有一个用于监测冷却剂流体温度的温度探头(17)。 格栅元件头(7)在其脚部(8)中具有向下开口的凹部(18),用作从下方供应的用于一次空气的空气逸出孔。