公开(公告)号：US20090312955A1

公开(公告)日：2009-12-17

申请号：US12482731

申请日：2009-06-11

申请人： Timothy Hutchins ,  Steven A. Scampini ,  Eric Baur

发明人： Timothy Hutchins ,  Steven A. Scampini ,  Eric Baur

IPC分类号： G06F19/00 ,  G01N1/28 ,  G01N15/08

CPC分类号： G01N1/36

摘要： A bubble point of a combination of a filter and a liquid is calibrated by applying an initial back pressure through the filter toward the liquid; taking a first plurality of measurements of the level of the liquid in the container; calculating a first variance of the first plurality of measurements; and comparing the first calculated variance with a known threshold variance. Then a second plurality of measurements of the level of the liquid in the container is taken; a second variance of the second plurality of measurements is calculated; and the second calculated variance is compared with a known threshold variance. The above described steps are repeated at incrementally increased back pressures until the first and second calculated variances are each greater than or equal to the known threshold variance, which is about 0.01. The bubble point is determined to be the back pressure at that point. If the determined bubble point is less than or equal to the known threshold bubble point, which is about 0.8 pounds per square inch, the filter is identified as unsuitable for use in cell block formation.

摘要翻译： 过滤器和液体的组合的起泡点通过将初始的背压通过过滤器朝向液体进行校准; 对容器中液体的水平进行第一次多次测量; 计算所述第一多个测量的第一方差; 以及将第一计算的方差与已知的阈值方差进行比较。 然后取出容器中的液体水平的第二多个测量值; 计算第二多个测量的第二方差; 并将第二计算方差与已知的阈值方差进行比较。 上述步骤以逐渐增加的背压重复，直到第一和第二计算的方差各自大于或等于已知阈值方差，其为约0.01。 泡点确定为此时的背压。 如果确定的气泡点小于或等于已知的阈值气泡点（约0.8磅/平方英寸），则过滤器被识别为不适合用于细胞块形成。

公开(公告)号：US20070134130A1

公开(公告)日：2007-06-14

申请号：US11298952

申请日：2005-12-09

申请人： Timothy Hutchins ,  Robert Sakal

发明人： Timothy Hutchins ,  Robert Sakal

IPC分类号： G01N33/00 ,  C12M1/34 ,  B01D61/00

CPC分类号： G01N1/2813 ,  G01N35/0099 ,  G01N2035/0405

摘要： A filtration based biological specimen collection and transfer system includes a vial cassette configured for accessibly storing a plurality of sample vials, a filter cassette configured for accessibly storing a plurality of filter cartridges, a slide cassette configured for accessibly storing a plurality of specimen slides, a specimen slide processor configured to receive a sample vial, a filter cartridge, and a specimen slide, a transport system including a movable arm having an effecter engagement mechanism; and a plurality of effecters. Each effecter is configured for engaging the movable arm, for retrieving the sample vials, the filter cartridges, or the specimen slides from the respective vial cassette, filter cassette, or slide cassette and positioning them in the specimen slide processor, and for retrieving the sample vials, the filter cartridges, or the specimen slides from the specimen slide processor.

摘要翻译： 基于过滤的生物样本收集和转移系统包括配置用于可容纳地存储多个样品瓶的小瓶盒，被配置用于可容纳地存储多个滤筒的过滤器盒，被配置用于可容纳地存储多个样本载玻片的载玻片盒， 样品滑块处理器，被配置为接收样品瓶，滤筒和样品载片，包括具有实施器接合机构的可动臂的输送系统; 和多个执行器。 每个执行器被构造成用于接合可移动臂，用于从相应的小瓶盒，过滤器盒或滑动盒取回样品瓶，过滤器滤芯或样品片，并将它们定位在样品滑块处理器中，并用于检索样品 样品瓶，过滤器滤芯或样品从试样滑块处理器上滑落。

公开(公告)号：US07829021B2

公开(公告)日：2010-11-09

申请号：US11839531

申请日：2007-08-15

申请人： Timothy Hutchins ,  Hal Watts ,  Steven Scampini ,  Eric Grimes ,  Andrew H. Fischer

发明人： Timothy Hutchins ,  Hal Watts ,  Steven Scampini ,  Eric Grimes ,  Andrew H. Fischer

IPC分类号： G01N21/00

CPC分类号： C12M47/00 ,  G01N1/36 ,  Y10T436/25 ,  Y10T436/2575

摘要： A system for making cell blocks includes a cell block cassette and processing station, the cassette including a main body having a having a collection aperture formed therein and a filter assembly removably attached to the main body, the filter assembly defining a collection well in communication with the collection aperture, and having a filter positioned across a bottom surface of the collection well, the filter configured to retain cellular matter carried in a fluid that is dispensed into the collection well and flows across the filter. The cell block processor has a cassette interface removably seating the cell block cassette, and a sensor positioned or positionable to detect and monitor a fluid level in the collection well. The processing station includes an automated fluid delivery system operable to dispense a fluid into the collection well, and a controller operatively coupled to the fluid delivery system, wherein the controller causes the fluid delivery system to selectively dispense fluids into the collection well based at least in part on a flow rate across the filter determined at least in part based on changes in the monitored fluid level in the collection well.

摘要翻译： 用于制造单元块的系统包括单元块盒和处理站，所述盒包括具有形成在其中的收集孔的主体和可移除地附接到主体的过滤器组件，所述过滤器组件限定与 收集孔，并且具有穿过收集阱的底表面定位的过滤器，所述过滤器被配置为保持携带在被分配到收集井中并流过过滤器的流体中的细胞物质。 细胞块处理器具有可移除地安置细胞块盒的盒式接口，以及定位或定位以便检测和监测收集阱中的液面的传感器。 处理站包括可操作以将流体分配到收集井中的自动化流体输送系统，以及可操作地耦合到流体输送系统的控制器，其中控制器使得流体输送系统选择性地将流体分配到收集井中， 至少部分地基于收集井中监测的液体水平的变化确定穿过过滤器的流速的部分。

公开(公告)号：US20060255289A1

公开(公告)日：2006-11-16

申请号：US11129647

申请日：2005-05-13

申请人： Thomas Cygan ,  Vincent Tsang ,  Raymond Burke ,  Richard Gill ,  Roy Martin ,  Timothy Hutchins

发明人： Thomas Cygan ,  Vincent Tsang ,  Raymond Burke ,  Richard Gill ,  Roy Martin ,  Timothy Hutchins

IPC分类号： G21K5/10

CPC分类号： H01J49/0495

摘要： In various aspects, the present teaching provide systems for providing sample ions, methods for providing sample ions, and sample support handling mechanisms. The sample support handling mechanisms of the present teachings comprising a sample support transfer mechanism portion and a sample support changing mechanism portion, where the sample support changing mechanism portion is disposed in a vacuum lock chamber. In various embodiments, the sample support handling mechanism facilitates providing consistent positioning of a sample support for subsequent ion generation by MALDI.

摘要翻译： 在各个方面，本教导提供用于提供样品离子的系统，用于提供样品离子的方法和样品支持处理机构。 本发明的样品支持处理机构包括样品支承转移机构部分和样品支撑体改变机构部分，其中样品支撑体改变机构部分设置在真空锁定室中。 在各种实施方案中，样品支持处理机制有助于提供样品载体的一致的定位，以便随后通过MALDI产生离子。

公开(公告)号：US08088329B2

公开(公告)日：2012-01-03

申请号：US12876879

申请日：2010-09-07

申请人： Timothy Hutchins ,  Hal Watts ,  Steven A. Scampini ,  Eric Grimes ,  Andrew H. Fischer

发明人： Timothy Hutchins ,  Hal Watts ,  Steven A. Scampini ,  Eric Grimes ,  Andrew H. Fischer

IPC分类号： G01N21/00

CPC分类号： C12M47/00 ,  G01N1/36 ,  Y10T436/25 ,  Y10T436/2575

摘要： A system for making cell blocks includes a cell block cassette and processing station, the cassette including a main body having a having a collection aperture formed therein and a filter assembly removably attached to the main body, the filter assembly defining a collection well in communication with the collection aperture, and having a filter positioned across a bottom surface of the collection well, the filter configured to retain cellular matter carried in a fluid that is dispensed into the collection well and flows across the filter. The cell block processor has a cassette interface removably seating the cell block cassette, and a sensor positioned or positionable to detect and monitor a fluid level in the collection well. The processing station includes an automated fluid delivery system operable to dispense a fluid into the collection well, and a controller operatively coupled to the fluid delivery system, wherein the controller causes the fluid delivery system to selectively dispense fluids into the collection well based at least in part on a flow rate across the filter determined at least in part based on changes in the monitored fluid level in the collection well.

摘要翻译： 用于制造单元块的系统包括单元块盒和处理站，所述盒包括具有形成在其中的收集孔的主体和可移除地附接到主体的过滤器组件，所述过滤器组件限定与 收集孔，并且具有穿过收集阱的底表面定位的过滤器，所述过滤器被配置为保持携带在被分配到收集井中并流过过滤器的流体中的细胞物质。 细胞块处理器具有可移除地安置细胞块盒的盒式接口，以及定位或定位以便检测和监测收集阱中的液面的传感器。 处理站包括可操作以将流体分配到收集井中的自动化流体输送系统，以及可操作地耦合到流体输送系统的控制器，其中控制器使得流体输送系统选择性地将流体分配到收集井中， 至少部分地基于收集井中监测的液体水平的变化确定穿过过滤器的流速的部分。

公开(公告)号：US07914462B2

公开(公告)日：2011-03-29

申请号：US11839528

申请日：2007-08-15

申请人： Timothy Hutchins ,  Hal Watts ,  Steven Scampini ,  Eric Grimes ,  Andrew H. Fischer

发明人： Timothy Hutchins ,  Hal Watts ,  Steven Scampini ,  Eric Grimes ,  Andrew H. Fischer

IPC分类号： A61B10/10 ,  B01L3/02 ,  C12M1/00 ,  G01N1/30

CPC分类号： C12M47/00 ,  G01N1/36 ,  Y10T436/25 ,  Y10T436/2575

摘要： A system for making cell blocks includes a cell block cassette and processing station, the cassette including a main body having a having a collection aperture formed therein and a filter assembly removably attached to the main body, the filter assembly defining a collection well in communication with the collection aperture, and having a filter positioned across a bottom surface of the collection well, the filter configured to retain cellular matter carried in a fluid that is dispensed into the collection well and flows across the filter. The cell block processor has a cassette interface removably seating the cell block cassette, and a sensor positioned or positionable to detect and monitor a fluid level in the collection well. The processing station includes an automated fluid delivery system operable to dispense a fluid into the collection well, and a controller operatively coupled to the fluid delivery system, wherein the controller causes the fluid delivery system to selectively dispense fluids into the collection well based at least in part on a flow rate across the filter determined at least in part based on changes in the monitored fluid level in the collection well.

摘要翻译： 用于制造单元块的系统包括单元块盒和处理站，所述盒包括具有形成在其中的收集孔的主体和可移除地附接到主体的过滤器组件，所述过滤器组件限定与 收集孔，并且具有穿过收集阱的底表面定位的过滤器，所述过滤器被配置为保持携带在被分配到收集井中并流过过滤器的流体中的细胞物质。 细胞块处理器具有可移除地安置细胞块盒的盒式接口，以及定位或定位以便检测和监测收集阱中的液面的传感器。 处理站包括可操作以将流体分配到收集井中的自动化流体输送系统，以及可操作地耦合到流体输送系统的控制器，其中控制器使得流体输送系统选择性地将流体分配到收集井中， 至少部分地基于收集井中监测的液体水平的变化确定穿过过滤器的流速的部分。

公开(公告)号：US20050133714A1

公开(公告)日：2005-06-23

申请号：US10742423

申请日：2003-12-19

申请人： Marvin Vestal ,  Timothy Hutchins

发明人： Marvin Vestal ,  Timothy Hutchins

IPC分类号： B01L20060101 ,  H01J49/04 ,  H01J49/16

CPC分类号： H01J49/0418

摘要： A MALDI plate construction is provided to support a sample in a manner to effect mass spectrometry of the sample. The plate construction comprises a sample receiving surface, a plate holder for retaining the sample receiving surface and an electrically conductive grid positioned adjacent to the sample receiving surface and in electrical contact with the plate holder.

摘要翻译： 提供了MALDI板结构以支持样品以进行样品的质谱分析。 板结构包括样品接收表面，用于保持样品接收表面的板保持器和邻近样品接收表面定位并与板保持器电接触的导电栅格。

公开(公告)号：US20100330660A1

公开(公告)日：2010-12-30

申请号：US12876879

申请日：2010-09-07

申请人： Timothy Hutchins ,  Hal Watts ,  Steven A. Scampini ,  Eric Grimes ,  Andrew H. Fischer

发明人： Timothy Hutchins ,  Hal Watts ,  Steven A. Scampini ,  Eric Grimes ,  Andrew H. Fischer

IPC分类号： C12M1/36

CPC分类号： C12M47/00 ,  G01N1/36 ,  Y10T436/25 ,  Y10T436/2575

摘要： A system for making cell blocks includes a cell block cassette and processing station, the cassette including a main body having a having a collection aperture formed therein and a filter assembly removably attached to the main body, the filter assembly defining a collection well in communication with the collection aperture, and having a filter positioned across a bottom surface of the collection well, the filter configured to retain cellular matter carried in a fluid that is dispensed into the collection well and flows across the filter. The cell block processor has a cassette interface removably seating the cell block cassette, and a sensor positioned or positionable to detect and monitor a fluid level in the collection well. The processing station includes an automated fluid delivery system operable to dispense a fluid into the collection well, and a controller operatively coupled to the fluid delivery system, wherein the controller causes the fluid delivery system to selectively dispense fluids into the collection well based at least in part on a flow rate across the filter determined at least in part based on changes in the monitored fluid level in the collection well.

摘要翻译： 用于制造单元块的系统包括单元块盒和处理站，所述盒包括具有形成在其中的收集孔的主体和可移除地附接到主体的过滤器组件，所述过滤器组件限定与 收集孔，并且具有穿过收集阱的底表面定位的过滤器，所述过滤器被配置为保持携带在被分配到收集井中并流过过滤器的流体中的细胞物质。 细胞块处理器具有可移除地安置细胞块盒的盒式接口，以及定位或定位以便检测和监测收集阱中的液面的传感器。 处理站包括可操作以将流体分配到收集井中的自动化流体输送系统，以及可操作地耦合到流体输送系统的控制器，其中控制器使得流体输送系统选择性地将流体分配到收集井中， 至少部分地基于收集井中监测的液体水平的变化确定穿过过滤器的流速的部分。

公开(公告)号：US20080081363A1

公开(公告)日：2008-04-03

申请号：US11839531

申请日：2007-08-15

申请人： Timothy Hutchins ,  Hal Watts ,  Steven Scampini ,  Eric Grimes ,  Andrew Fischer

发明人： Timothy Hutchins ,  Hal Watts ,  Steven Scampini ,  Eric Grimes ,  Andrew Fischer

IPC分类号： C12M3/04

CPC分类号： C12M47/00 ,  G01N1/36 ,  Y10T436/25 ,  Y10T436/2575

摘要： A system for making cell blocks includes a cell block cassette and processing station, the cassette including a main body having a having a collection aperture formed therein and a filter assembly removably attached to the main body, the filter assembly defining a collection well in communication with the collection aperture, and having a filter positioned across a bottom surface of the collection well, the filter configured to retain cellular matter carried in a fluid that is dispensed into the collection well and flows across the filter. The cell block processor has a cassette interface removably seating the cell block cassette, and a sensor positioned or positionable to detect and monitor a fluid level in the collection well. The processing station includes an automated fluid delivery system operable to dispense a fluid into the collection well, and a controller operatively coupled to the fluid delivery system, wherein the controller causes the fluid delivery system to selectively dispense fluids into the collection well based at least in part on a flow rate across the filter determined at least in part based on changes in the monitored fluid level in the collection well.

摘要翻译： 用于制造单元块的系统包括单元块盒和处理站，所述盒包括具有形成在其中的收集孔的主体和可移除地附接到主体的过滤器组件，所述过滤器组件限定与 收集孔，并且具有穿过收集阱的底表面定位的过滤器，所述过滤器被配置为保持携带在被分配到收集井中并流过过滤器的流体中的细胞物质。 细胞块处理器具有可移除地安置细胞块盒的盒式接口，以及定位或定位以便检测和监测收集阱中的液面的传感器。 处理站包括可操作以将流体分配到收集井中的自动化流体输送系统，以及可操作地耦合到流体输送系统的控制器，其中控制器使得流体输送系统选择性地将流体分配到收集井中， 至少部分地基于收集井中监测的液体水平的变化确定穿过过滤器的流速的部分。

公开(公告)号：US20080081351A1

公开(公告)日：2008-04-03

申请号：US11839528

申请日：2007-08-15

申请人： Timothy Hutchins ,  Hal Watts ,  Steven Scampini ,  Eric Grimes ,  Andrew Fischer

发明人： Timothy Hutchins ,  Hal Watts ,  Steven Scampini ,  Eric Grimes ,  Andrew Fischer

IPC分类号： C12Q1/24

CPC分类号： C12M47/00 ,  G01N1/36 ,  Y10T436/25 ,  Y10T436/2575

摘要： A system for making cell blocks includes a cell block cassette and processing station, the cassette including a main body having a having a collection aperture formed therein and a filter assembly removably attached to the main body, the filter assembly defining a collection well in communication with the collection aperture, and having a filter positioned across a bottom surface of the collection well, the filter configured to retain cellular matter carried in a fluid that is dispensed into the collection well and flows across the filter. The cell block processor has a cassette interface removably seating the cell block cassette, and a sensor positioned or positionable to detect and monitor a fluid level in the collection well. The processing station includes an automated fluid delivery system operable to dispense a fluid into the collection well, and a controller operatively coupled to the fluid delivery system, wherein the controller causes the fluid delivery system to selectively dispense fluids into the collection well based at least in part on a flow rate across the filter determined at least in part based on changes in the monitored fluid level in the collection well.

摘要翻译： 用于制造单元块的系统包括单元块盒和处理站，所述盒包括具有形成在其中的收集孔的主体和可移除地附接到主体的过滤器组件，所述过滤器组件限定与 收集孔，并且具有穿过收集阱的底表面定位的过滤器，所述过滤器被配置为保持携带在被分配到收集井中并流过过滤器的流体中的细胞物质。 细胞块处理器具有可移除地安置细胞块盒的盒式接口，以及定位或定位以便检测和监测收集阱中的液面的传感器。 处理站包括可操作以将流体分配到收集井中的自动化流体输送系统，以及可操作地耦合到流体输送系统的控制器，其中控制器使得流体输送系统选择性地将流体分配到收集井中， 至少部分地基于收集井中监测的液体水平的变化确定穿过过滤器的流速的部分。