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公开(公告)号：US07888123B2
公开(公告)日：2011-02-15
申请号：US11413173
申请日：2006-04-28
申请人： Kazuhisa Ono , Tsunehiro Aki , Seiji Kawamoto
发明人： Kazuhisa Ono , Tsunehiro Aki , Seiji Kawamoto
摘要： An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.
摘要翻译： 本发明的一个目的是提供一种能够阐明脂质如PUFA和类胡萝卜素的生物合成机理的Labyrinthuloycycota的转化系统，以及构建高生产系统以及新型功能性脂质分子的设计和开发 机制的控制。 本发明提供了将转基因导入到迷路神经霉素细胞中的方法，其包括将包含转基因和核苷酸序列的重组载体导入到迷路形霉菌属细胞中，该载体与Labyrinthuloycycota的一部分染色体DNA同源且能够同源 与染色体DNA重组，然后在该同源核苷酸序列中诱导同源重组。
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2.发明授权Microorganism having an ability of producing docosahexaenoic acid and use thereof 有权具有生产二十二碳六烯酸的能力的微生物及其用途公开(公告)号：US07259006B2
公开(公告)日：2007-08-21
申请号：US10743832
申请日：2003-12-24
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , C11C1/025 , C11C1/045 , C11C3/123 , C12P7/6472 , C12R1/90
摘要： An object of the present invention is to provide a microorganism which has a high ability of producing docosahexaenoic acid. The present invention provides a Thraustochytrium strain which has an ability of producing docosahexaenoic acid, and use thereof.
摘要翻译： 本发明的目的是提供一种具有高生产二十二碳六烯酸能力的微生物。 本发明提供了具有生产二十二碳六烯酸的能力的破囊壶菌菌株及其应用。
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3.发明授权Method for producing highly unsaturated fatty acids and lipid containing same 有权生产高度不饱和脂肪酸的方法和含有它的脂质公开(公告)号：US06958229B2
公开(公告)日：2005-10-25
申请号：US09389318
申请日：1999-09-03
申请人： Osamu Suzuki , Kazuhisa Ono , Seiko Shigeta , Tsunehiro Aki , Kengo Akimoto
发明人： Osamu Suzuki , Kazuhisa Ono , Seiko Shigeta , Tsunehiro Aki , Kengo Akimoto
CPC分类号： C12P7/6427 , A23K10/16 , A23K20/158 , C12P7/6472
摘要： A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g/L of highly unsaturated fatty acids.
摘要翻译： 一种生产高度不饱和脂肪酸的方法，包括在碳源浓度至少为4％（重量）的培养基中培养属于被孢霉属（Mortierella）的微生物并具有耐碳源性的微生物，并从 培养产品。 将微生物培养约一周，得到至少约7g / L的高度不饱和脂肪酸。
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4.发明授权Microorganism having an ability of producing docosahexaenoic acid and use thereof 有权具有生产二十二碳六烯酸的能力的微生物及其用途公开(公告)号：US07863026B2
公开(公告)日：2011-01-04
申请号：US11774261
申请日：2007-07-06
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , C11C1/025 , C11C1/045 , C11C3/123 , C12P7/6472 , C12R1/90
摘要： An object of the present invention is to provide a microorganism which has a high ability of producing docosahexaenoic acid. The present invention provides a Thraustochytrium strain which has an ability of producing docosahexaenoic acid, and use thereof.
摘要翻译： 本发明的目的是提供一种具有高生产二十二碳六烯酸能力的微生物。 本发明提供了具有生产二十二碳六烯酸的能力的破囊壶菌菌株及其应用。
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公开(公告)号：US07863024B2
公开(公告)日：2011-01-04
申请号：US10512557
申请日：2003-04-25
申请人： Kazuhisa Ono , Tsunehiro Aki , Kenichi Higashiyama
发明人： Kazuhisa Ono , Tsunehiro Aki , Kenichi Higashiyama
CPC分类号： C12P7/6472
摘要： The present invention provides a process for producing a highly unsaturated fatty acid-containing lipid which is less expensive than existing ones, which comprises culturing a microorganism belonging to the genus Mortierella with the use of, as a medium carbon source, a saccharified starch, which is less expensive than glucose, does not contribute to an increase in osmotic pressure of the culture medium, and can be utilized by the Mortierella microorganism, and collecting a highly unsaturated fatty acid-containing lipid from the culture.
摘要翻译： 本发明提供了一种生产高度不饱和脂肪酸的脂质的方法，其比现有的脂肪酸便宜，其包括使用作为中等碳源来培养属于被孢霉属的微生物的糖化淀粉，其中糖化淀粉 比葡萄糖便宜，不会造成培养基的渗透压升高，可被被孢霉属微生物利用，并从培养物中收集高度不饱和脂肪酸的脂质。
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公开(公告)号：US07759097B2
公开(公告)日：2010-07-20
申请号：US11413141
申请日：2006-04-28
申请人： Kazuhisa Ono , Tsunehiro Aki , Seiji Kawamoto
发明人： Kazuhisa Ono , Tsunehiro Aki , Seiji Kawamoto
CPC分类号： C12P7/6463 , C12N15/79 , C12P7/6409
摘要： An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a vector for the transformation of Labyrinthulomycota with a transgene, which comprises at least (1) a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream, and (3) a cloning site for transgene insertion having a promoter sequence located upstream and a terminator sequence located downstream.
摘要翻译： 本发明的一个目的是提供一种能够阐明脂质如PUFA和类胡萝卜素的生物合成机理的Labyrinthuloycycota的转化系统，以及构建高生产系统以及新型功能性脂质分子的设计和开发 机制的控制。 本发明提供了用转基因转化迷路孢霉菌纲的载体，其包含至少（1）与Labyrinthuloycycota的一部分染色体DNA同源且能够与染色体DNA同源重组的核苷酸序列，（2） 具有位于上游的启动子序列和位于下游的终止子序列的选择标记基因，和（3）位于上游的位于上游的启动子序列和位于下游的终止子序列的转基因插入的克隆位点。
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7.发明申请MICROORGANISM HAVING AN ABILITY OF PRODUCING DOCOSAHEXAENOIC ACID AND USE THEREOF 有权具有生产DOCOSAHEXAENOIC酸的能力的微生物及其使用公开(公告)号：US20080009045A1
公开(公告)日：2008-01-10
申请号：US11774261
申请日：2007-07-06
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , C11C1/025 , C11C1/045 , C11C3/123 , C12P7/6472 , C12R1/90
摘要： An object of the present invention is to provide a microorganism which has a high ability of producing docosahexaenoic acid. The present invention provides a Thraustochytrium strain which has an ability of producing docosahexaenoic acid, and use thereof.
摘要翻译： 本发明的目的是提供一种具有高生产二十二碳六烯酸能力的微生物。 本发明提供了具有生产二十二碳六烯酸的能力的破囊壶菌菌株及其应用。
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公开(公告)号：US20060286650A1
公开(公告)日：2006-12-21
申请号：US11413141
申请日：2006-04-28
申请人： Kazuhisa Ono , Tsunehiro Aki , Seiji Kawamoto
发明人： Kazuhisa Ono , Tsunehiro Aki , Seiji Kawamoto
CPC分类号： C12P7/6463 , C12N15/79 , C12P7/6409
摘要： An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a vector for the transformation of Labyrinthulomycota with a transgene, which comprises at least (1) a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream, and (3) a cloning site for transgene insertion having a promoter sequence located upstream and a terminator sequence located downstream.
摘要翻译： 本发明的一个目的是提供一种能够阐明脂质如PUFA和类胡萝卜素的生物合成机理的Labyrinthuloycycota的转化系统，以及构建高生产系统以及新型功能性脂质分子的设计和开发 机制的控制。 本发明提供了用转基因转化迷路孢霉菌纲的载体，其包含至少（1）与Labyrinthuloycycota的一部分染色体DNA同源且能够与染色体DNA同源重组的核苷酸序列，（2） 具有位于上游的启动子序列和位于下游的终止子序列的选择标记基因，和（3）位于上游的位于上游的启动子序列和位于下游的终止子序列的转基因插入的克隆位点。
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公开(公告)号：US20060275904A1
公开(公告)日：2006-12-07
申请号：US11413173
申请日：2006-04-28
申请人： Kazuhisa Ono , Tsunehiro Aki , Seiji Kawamoto
发明人： Kazuhisa Ono , Tsunehiro Aki , Seiji Kawamoto
摘要： An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.
摘要翻译： 本发明的一个目的是提供一种能够阐明脂质如PUFA和类胡萝卜素的生物合成机理的Labyrinthuloycycota的转化系统，以及构建高生产系统以及新型功能性脂质分子的设计和开发 机制的控制。 本发明提供了将转基因导入到迷路神经霉素细胞中的方法，其包括将包含转基因和核苷酸序列的重组载体导入到迷路形霉菌属细胞中，该载体与Labyrinthuloycycota的一部分染色体DNA同源且能够同源 与染色体DNA重组，然后在该同源核苷酸序列中诱导同源重组。
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10.发明申请Method for producing highly unsaturated fatty acids and lipid containing same 审中-公开生产高度不饱和脂肪酸的方法和含有它的脂质公开(公告)号：US20060014268A1
公开(公告)日：2006-01-19
申请号：US11232037
申请日：2005-09-22
申请人： Osamu Suzuki , Kazuhisa Ono , Seiko Shigeta , Tsunehiro Aki , Kengo Akimoto
发明人： Osamu Suzuki , Kazuhisa Ono , Seiko Shigeta , Tsunehiro Aki , Kengo Akimoto
CPC分类号： C12P7/6427 , A23K10/16 , A23K20/158 , C12P7/6472
摘要： A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g/L of highly unsaturated fatty acids.
摘要翻译： 一种生产高度不饱和脂肪酸的方法，包括在碳源浓度至少为4％（重量）的培养基中培养属于被孢霉属（Mortierella）的微生物并具有耐碳源性的微生物，并从 培养产品。 将微生物培养约一周，得到至少约7g / L的高度不饱和脂肪酸。
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