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    Method for Identifying Allergenic Proteins And Peptides
    8.
    发明申请
    Method for Identifying Allergenic Proteins And Peptides 有权
    识别过敏原蛋白和肽的方法

    公开(公告)号：US20100143262A1

    公开(公告)日：2010-06-10

    申请号：US12581108

    申请日：2009-10-16

    申请人： Rudolph Valenta ,  Ric Van Tol ,  Udo Herz ,  Heidrun Hochwallner ,  Margarte Focke-Tejkl ,  Ines Swoboda ,  Ulrike Schulmeister

    发明人： Rudolph Valenta ,  Ric Van Tol ,  Udo Herz ,  Heidrun Hochwallner ,  Margarte Focke-Tejkl ,  Ines Swoboda ,  Ulrike Schulmeister

    IPC分类号： A61K49/00 ,  C40B30/06 ,  C07K7/08 ,  C07K14/00 ,  A61K38/08 ,  A61K38/10 ,  A61K38/16 ,  C07K7/06 ,  G01N33/53 ,  A61P37/06

    CPC分类号： G01N33/6854 ,  G01N2500/00 ,  G01N2800/24

    摘要： A method for identifying allergenic proteins and peptides. More specifically, a method for identifying allergenic milk proteins and/or peptides including the steps of: providing at least one expression library comprising DNA or cDNA derived from the mammary gland tissue of a lactating cow, expressing at least one protein or peptide encoded by said expression library, determining the binding capacity of said at least one protein or peptide to IgE of at least one serum of an individual who is sensitive to cow's milk, contacting the at least one protein or peptide exhibiting an IgE binding capacity as determined in step c) with basophil cells, eosinophil cells or mast cells and identifying the at least one protein or peptide as being allergenic when said basophil cells, eosinophil cells or mast cells release upon contact with at least one protein or peptide of step d) at least one mediator.

    摘要翻译： 识别过敏原蛋白和肽的方法。 更具体地，涉及一种用于鉴定过敏性乳蛋白和/或肽的方法，包括以下步骤：提供至少一种表达文库，其包含源自哺乳动物牛乳腺组织的DNA或cDNA，其表达至少一种由所述 表达文库，测定所述至少一种蛋白质或肽与至少一种对牛奶敏感的个体的血清的IgE的结合能力，使与所述至少一种表现IgE结合能力的蛋白质或肽接触，如步骤c ），嗜碱性粒细胞，嗜酸性粒细胞或肥大细胞，并且当所述嗜碱性粒细胞，嗜酸性粒细胞或肥大细胞与步骤d）的至少一种蛋白质或肽接触时释放时，将至少一种蛋白质或肽鉴定为过敏原。至少一种介体 。