公开(公告)号：US20240287506A1

公开(公告)日：2024-08-29

申请号：US18572473

申请日：2022-05-31

申请人： WESTLAKE UNIVERSITY

发明人： Zhike LU ,  Lijia MA

IPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  C12Q1/686

CPC分类号： C12N15/1068 ,  C12N9/22 ,  C12N15/1096 ,  C12N15/11 ,  C12N15/86 ,  C12Q1/686 ,  C12N2310/20 ,  C12N2740/15043

摘要： Provided is a library construction method based on long overhang sequence ligation, a library constructed by the library construction method, for example, a CRISPR library of pair-specific multiplexed guide RNA (gRNA) combinations, and a use of the library. The CRISPR library of pair-specific multiplexed gRNA combinations can be used to simultaneously perturb multiple (e.g., 4) pre-designed targets in CRISPR/Cas9 screening. The CRISPR library of pair-specific multiplexed gRNA combinations can be a powerful tool for studying the combinatorial outcomes from coordinated gene behaviors.

公开(公告)号：US20240092474A1

公开(公告)日：2024-03-21

申请号：US18038220

申请日：2020-11-23

申请人： WESTLAKE UNIVERSITY

发明人： Bing LUO ,  Wei LI ,  Weicheng CUI

IPC分类号： B64C3/48

CPC分类号： B64C3/48

摘要： Embodiments of the present disclosure provide a wing element structure for an aircraft, comprising a plurality of wing element units, wherein adjacent wing element units are connected by airfoil control units, hydraulic units and wing element outer edge connecting members, each of the wing element units comprises a plurality of wing element edge members connected to one another and a wing support rod connecting member, and the wing support rod connecting member located in a wing leading edge in the wing element unit is connected to a support rod structure on an aircraft main body. The embodiments of the present disclosure provide a wing element structure having a wide range of morphing airfoil features in terms of chord length and curvature; the embodiments of present disclosure have the capability of morphing airfoil and variable pitch angle in a wide range, and can make adjustment regarding complex flow fields or environments, which greatly improves the movement speed and movement efficiency and can achieve high-mobility actions.

公开(公告)号：US20230287419A1

公开(公告)日：2023-09-14

申请号：US18043874

申请日：2020-09-30

申请人： WESTLAKE UNIVERSITY

发明人： Xing CHANG ,  Jia LI ,  Han QIU

IPC分类号： C12N15/113 ,  C12N15/86 ,  C07K14/47 ,  A61P21/00

CPC分类号： C12N15/113 ,  A61P21/00 ,  C07K14/4708 ,  C12N15/86 ,  C12N2750/14143

摘要： A duchenne muscular dystrophy-related exonic splicing enhancer, sgRNA and gene editing tool can be applied in the preparation of drugs for treating duchenne muscular dystrophy. The gene editing tool designed on the basis of cytosine deaminase AID mutants and Cas9 mutants can perform site-specific modification on a mammalian genome by using an adeno-associated virus (AAV) as a vector. By optimizing an encoding nucleic acid sequence and an element composition structure of the editing tool, site-specific targeted modification of mammalian genetic material DNA can be efficiently achieved; and by performing targeted genetic manipulation on the nucleic acid sequence carrying disease mutations, a pathogenic mutation cannot be retained in a mature protein amino acid sequence or the pathogenic mutation cannot perform its function, so that the purpose of treating various gene mutation type genetic rare diseases is achieved, and the advantages of high efficiency, safety and stability are achieved.

公开(公告)号：US11885947B2

公开(公告)日：2024-01-30

申请号：US17289554

申请日：2019-07-23

申请人： WESTLAKE UNIVERSITY

发明人： Liang Gao ,  Bi-Chang Chen

IPC分类号： G01N21/64 ,  G02B21/00 ,  G02B21/14

CPC分类号： G02B21/0032 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/14

摘要： A lattice light sheet microscope includes a single spatial light modulator (SLM), the optical modulation plane of which conjugates to an image plane of an excitation objective and which is configured to: generate an optical lattice by loading a phase map obtained from a central cross section of a corresponding optical lattice of a desired lattice light sheet, and tile the optical lattice by loading a phase map obtained from an off-center cross section of the corresponding optical lattice. The lattice light sheet microscope also includes a transparent annular diaphragm arranged at a plane conjugating to the entrance pupil of the excitation objective lens. The lattice light sheet microscope further includes a first galvanometer configured to: scan each optical lattice in the extending direction thereof so as to form a tiled lattice light sheet.

公开(公告)号：US20230194843A1

公开(公告)日：2023-06-22

申请号：US17772688

申请日：2020-04-10

申请人： WESTLAKE UNIVERSITY

发明人： Liang GAO

IPC分类号： G02B21/00 ,  G02B21/06 ,  G02B21/36

CPC分类号： G02B21/0032 ,  G02B21/06 ,  G02B21/0036 ,  G02B21/367

摘要： Provided are a tiling light sheet selective plane illumination microscope (TLS-SPIM), a use method thereof and a microscope system. The use method includes loading a corresponding phase map to each group of pupil subsections of a pupil by a spatial light modulator, and performing phase modulation on an excitation beam to create at least two coaxial excitation beam arrays scanning the created at least two coaxial excitation beam arrays to generate discontinuous light sheets accordingly; and tiling at least one of the generated discontinuous light sheets in the propagation direction of the excitation light to obtain tiling light sheets for selective plane illumination of a sample. The method of using the TLS-SPIM, the TLS-SPIM and the system including same enables significant increasing of imaging speed, improvement of resolution, and reduction of source data amount.

公开(公告)号：US20220284989A1

公开(公告)日：2022-09-08

申请号：US17597648

申请日：2020-11-10

申请人： Westlake University

发明人： Tiannan Guo ,  Zhongzhi Luan ,  Ziqing Li ,  Fangfei Zhang ,  Shaoyang Yu ,  Zelin Zang

IPC分类号： G16C20/20 ,  G16C20/70 ,  G16C20/80 ,  G01N27/62

摘要： The present invention relates to the technical field of biomolecular omics mass spectrometry data, in particular to an implementation method of a molecular omics data structure based on data independent acquisition mass spectra. The mass spectrometry data structure is DIAT (Data-Independent Acquisition Tensor) data generated from original mass spectrometry data and has attributes of three dimensions, the first dimension is a cycle index, the second dimension is a fragment ion mass-to-charge ratio, and the third dimension is a precursor ion window index corresponding to a fragment ion. The DIAT data of this solution is high in integrity, convenient to read and high in reading speed, and the size of a DIAT file is only a few tenths of that of an mzXML file. DIA mass spectrometry data can be directly observed through a visualized pooled DIAT file image, and a DIAT can be analyzed by directly using a visual processing algorithm, which avoids the operation of extracting ion chromatographic with a large amount of calculation and can directly establish a computer deep learning model for clinical phenotype classification and prediction according to the file.

公开(公告)号：US20240358895A1

公开(公告)日：2024-10-31

申请号：US18648289

申请日：2024-04-26

申请人： Westlake University

发明人： Chuanqing WANG ,  Jie YANG ,  MOHAMAD SAWAN

IPC分类号： A61L27/36 ,  G06V10/774 ,  G06V20/40

CPC分类号： A61L27/3695 ,  A61L27/3604 ,  A61L27/3675 ,  G06V10/774 ,  G06V20/46 ,  A61L2430/16

摘要： Disclosed are a model training method, a visual perception method, an electronic device and a storage medium. The model training method is applicable to a spiking recurrent model in a retinal prosthesis and includes: determining labels respectively corresponding to ganglion cells based on a preset ganglion cell response dataset; obtaining multiple spike signals as training samples; inputting the spike signals into the spiking recurrent model, obtaining spike responses of the ganglion cells predicted by the spiking recurrent model, and computing a loss value based on the spike responses of the ganglion cells, the labels and a preset Poisson loss function; and updating a weight for each layer in the spiking recurrent model based on the loss value and a preset time backpropagation function recursively until the spiking recurrent model converges, thereby scientifically and quickly training the spiking recurrent model for predicting the responses of retinal ganglion cells.

公开(公告)号：US20240344939A1

公开(公告)日：2024-10-17

申请号：US18294470

申请日：2022-03-09

申请人： WESTLAKE UNIVERSITY

发明人： Liang Gao ,  Ruili Feng ,  Yanlu Chen

IPC分类号： G01N1/28

CPC分类号： G01N1/28

摘要： A degreasing composition for clearing biological tissue, a use in the preparation of a degreasing reagent, a degreasing reagent, a clearing kit comprising the degreasing composition or the degreasing reagent, a biological tissue clearing treatment method using the degreasing reagent, and an imaging method for clearing biological tissue comprising the steps of the biological tissue clearing treatment method. The degreasing composition comprises urea, N-butyldiethanolamine and Triton X-100, wherein the mass ratio of urea, N-butyldiethanolamine and Triton X-100 is (0.6-5):(0.3-3):1. The degreasing composition has a fast degreasing speed, good degreasing capabilities for large-volume biological samples, and may retain the structural features of biological tissues well from the cellular level to the subcellular level.

公开(公告)号：US20240293991A1

公开(公告)日：2024-09-05

申请号：US18284847

申请日：2022-12-22

申请人： WESTLAKE UNIVERSITY

发明人： Hanqing Jiang ,  Zhuang Zhang ,  Luoqian Emu ,  Zhenghao Xu

IPC分类号： B31D5/04 ,  B25J13/02 ,  G06F3/01

CPC分类号： B31D5/04 ,  B25J13/025 ,  G06F3/014 ,  G06F3/016

摘要： Curved origami-based meta-material, manufacturing method of the same, curved origami-based haptic module and a method for producing active mechanical haptics. The curved origami-based metamaterial includes a panel having a single curved crease extending in its longitudinal direction, which divides the panel into two facets at its two opposite sides. The panel is configured to be folded along the single curved crease to form a folding angle between the two facets. The folding angle is configured to be adjustable to achieve a variable stiffness when loaded in a vertical direction of the folded panel, covering positive stiffness to negative stiffness.

公开(公告)号：US20240241449A1

公开(公告)日：2024-07-18

申请号：US18622477

申请日：2024-03-29

申请人： WESTLAKE UNIVERSITY

发明人： Xijun LI ,  Chunyan SONG

IPC分类号： G03F7/00

CPC分类号： G03F7/70291 ,  G03F7/70508 ,  G03F7/70725

摘要： A lithographing apparatus and a lithographing system. The lithographing apparatus comprises: a signal generation module (110) configured to generate one or more mask patterns according to a layout, and to generate a respective mask driving signal according to each of the one or more mask patterns respectively; and a mask driving module (120) communicatively connected with the signal generation module (110) and configured to control a light-transmitting state of each pixel region on a mask (200) according to one or more sets of mask driving signals respectively, to form a respective mask pattern on the mask (200).