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12 条记录 (耗时 0.015 秒)

    Cell cultivating flask
    1.
    发明授权
    Cell cultivating flask 有权
    细胞培养瓶

    公开(公告)号：US07078228B2

    公开(公告)日：2006-07-18

    申请号：US10750474

    申请日：2003-12-31

    申请人： William J. Lacey ,  John A. Ryan ,  Laurence M. Vaughan ,  Joseph C. Wall ,  Kathy M. Youngbear

    发明人： William J. Lacey ,  John A. Ryan ,  Laurence M. Vaughan ,  Joseph C. Wall ,  Kathy M. Youngbear

    IPC分类号： C12M1/34

    CPC分类号： C12M23/24 ,  C12M23/08 ,  C12M29/20

    摘要： A stackable flask for the culturing of cells is disclosed. The cell culture chamber is defined by a top plate and a rigid bottom tray of substantially rectangular shape connected by side and end walls, the body of the flask has imparted therein a gas permeable membrane that will allow the free flow of gases between the cell culture chamber and the external environment. The flask body also includes a sealed septum that will allow access to the cell growth chamber by means of a needle or cannula. The size of the flask and location of an optional neck and cap section allows for flask manipulation by standard automated assay equipment, making the flask ideal for high throughput applications.

    摘要翻译： 公开了一种用于培养细胞的可堆叠烧瓶。 细胞培养室由顶板和由侧壁和端壁连接的基本矩形形状的刚性底托盘限定，烧瓶的主体在其中赋予气体渗透膜，其允许气体在细胞培养物之间自由流动 室和外部环境。 烧瓶主体还包括密封的隔膜，其将允许通过针或插管进入细胞生长室。 烧瓶的尺寸和可选的颈部和帽部分的位置允许通过标准自动化测定设备进行烧瓶操作，使烧瓶适合于高通量应用。

    Non-rigid universal coupling for health related equipment
    2.
    发明授权
    Non-rigid universal coupling for health related equipment 失效
    健康相关设备的非刚性通用联轴器

    公开(公告)号：US4484769A

    公开(公告)日：1984-11-27

    申请号：US446662

    申请日：1983-03-09

    申请人： William J. Lacey

    发明人： William J. Lacey

    IPC分类号： F16L37/04 ,  F16L25/00

    CPC分类号： A61M39/12 ,  F16L37/04 ,  Y10S285/921

    摘要： An expendable adaptor for quick coupling flexible hoses to a variety of devices such as oxygen breathing equipment and including an enlarged deformable head portion having at least a pair of concentric sleeves the inner of which is axially recessed so that its forward face engages an end edge of a complementary coupling fitting in sealing relationship while the outer sleeve surrounds the fitting in a secondary sealing as well as anchoring relationship.

    摘要翻译： 一种用于将柔性软管快速连接到诸如氧气呼吸设备的各种装置的消耗式适配器，并且包括具有至少一对同心套筒的扩大的可变形头部部分，其内部轴向凹入，使得其前表面接合 一个互补的联接装置的密封关系，而外套筒在二次密封以及锚固关系中包围配件。

    Multi-well assembly for growing cultures in-vitro
    4.
    发明授权
    Multi-well assembly for growing cultures in-vitro 失效
    用于体外培养培养物的多孔组装

    公开(公告)号：US06943009B2

    公开(公告)日：2005-09-13

    申请号：US10146831

    申请日：2002-05-15

    申请人： William J. Lacey ,  Gregory Mathus ,  David M. Root ,  John A. Ryan

    发明人： William J. Lacey ,  Gregory Mathus ,  David M. Root ,  John A. Ryan

    IPC分类号： B01L3/00 ,  B29C33/00 ,  B29C33/44 ,  B29C33/76 ,  C12M1/32 ,  C12M1/12

    CPC分类号： B01L3/50853 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/0829 ,  B29C33/0033 ,  B29C33/44 ,  B29C33/76 ,  C12M21/06 ,  C12M23/12 ,  C12M23/24

    摘要： A high-throughput cell or tissue culture apparatus, which is configurable to an industry-standard well plate format, is provided. The apparatus comprises a number of vessels, which may be suspended in wells of a plate. Each vessel has at least one sidewall defining a first opening and a second opening, each of predetermined cross-sectional area. The second opening has an inner cross-sectional area greater than either the inner cross-sectional area of the first opening or a cross-sectional area in a horizontal plane between the first and second openings. A relatively large substrate area is provided in each vessel for supporting tissue cultures in a fluid medium.

    摘要翻译： 提供了可配置为工业标准孔板格式的高通量细胞或组织培养装置。 该装置包括许多容器，其可以悬浮在板的孔中。 每个容器具有限定第一开口和第二开口的至少一个侧壁，每个预定横截面积。 第二开口具有大于第一开口的内截面积或第一和第二开口之间的水平平面中的横截面积的内横截面面积。 在每个容器中提供相对大的衬底面积，用于支持流体介质中的组织培养。

    Method of making microplates with UV permeable bottom wells
    5.
    发明授权
    Method of making microplates with UV permeable bottom wells 有权
    用UV透过性底孔制作微孔板的方法

    公开(公告)号：US6103169A

    公开(公告)日：2000-08-15

    申请号：US153134

    申请日：1998-09-15

    申请人： Gregory Mathus ,  Paul M. Szlosek ,  William J. Lacey

    发明人： Gregory Mathus ,  Paul M. Szlosek ,  William J. Lacey

    IPC分类号： G01N1/00 ,  B01L3/00 ,  B29C45/14 ,  B29D99/00 ,  B29K105/32 ,  B29L22/00 ,  B29L31/00 ,  B32B27/30 ,  C08L27/12 ,  C12M1/32 ,  G01N1/10 ,  G01N21/03 ,  G01N21/25 ,  G01N21/33

    CPC分类号： B01L3/5085 ,  B29C45/1418 ,  B29C45/14344 ,  B29C45/14778 ,  G01N21/03 ,  G01N21/25 ,  G01N21/33 ,  B01L2300/0829 ,  B01L2300/12

    摘要： Microplates and methods for manufacturing microplates. The microplate is designed to allow UV radiation to pass through the bottom wells of the microplate so that the microplate can be used for assaying samples by use of UV absorbance. In one embodiment, the microplate comprises at least first and second wells, each well having a UV permeable bottom. In another embodiment, the microplate comprises a frame having an upper portion and a lower portion contiguous with the upper portion and a sheet disposed between the upper portion and the lower portion and defining the bottom of at least one well of the microplate. One embodiment of the method includes steps of inserting a sheet of UV permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, injecting molten plastic material into the mold cavity, and cooling the plastic material to form the microplate with the plastic material forming the sidewalls of each of the first and second wells and the sheet of UV permeable material forming the bottom of each of the first and second wells. Another embodiment of the method includes providing an upper plate defining sidewalls of at least one well, adhering an intermediate layer to the upper plate and adhering a sheet of UV permeable material to the intermediate layer. A further embodiment of the method includes inserting a sheet of material having at least one hole into a mold cavity, injecting a molten plastic material into the mold cavity and cooling the plastic material to form a microplate.

    摘要翻译： 微孔板和微孔板制造方法。 微孔板被设计成允许紫外线辐射通过微板的底部孔，使得微孔板可以用于通过使用UV吸光度来测定样品。 在一个实施方案中，微板包括至少第一和第二孔，每个孔具有可UV透过的底部。 在另一个实施例中，微孔板包括具有与上部邻接的上部和下部的框架，以及设置在上部和下部之间并限定微板的至少一个孔的底部的框架。 该方法的一个实施例包括以下步骤：将一片UV可渗透材料插入到模腔中，该模腔包括成形为形成多个孔的侧壁的部分，将熔融的塑料材料注入模腔中，并冷却塑料材料以形成 微板与塑料材料形成第一和第二阱中的每一个的侧壁以及形成第一和第二孔中每一个的底部的UV透过材料片。 该方法的另一实施例包括提供限定至少一个阱的侧壁的上板，将中间层粘附到上板并将一片UV可渗透材料粘合到中间层。 该方法的另一实施例包括将具有至少一个孔的材料片插入模腔中，将熔融的塑料材料注入模腔并冷却塑料以形成微板。

    Multilayer tissue culture vessel
    6.
    发明授权
    Multilayer tissue culture vessel 有权
    多层组织培养容器

    公开(公告)号：US08778669B2

    公开(公告)日：2014-07-15

    申请号：US12840758

    申请日：2010-07-21

    申请人： William J. Lacey ,  Michael S. Shanler ,  Amy W. Cai

    发明人： William J. Lacey ,  Michael S. Shanler ,  Amy W. Cai

    IPC分类号： C12M1/00 ,  C12M3/00 ,  C12M1/12 ,  C12M1/24

    CPC分类号： C12M25/06 ,  C12M23/04 ,  C12M23/08 ,  C12M23/28 ,  C12M23/34 ,  C12M23/38 ,  C12M23/40 ,  C12M23/44 ,  C12M25/04 ,  C12M25/14

    摘要： The present invention discloses a vessel for culturing cells which includes: a bottom including a base with an upwardly extending wall at least partially bounding the base of the bottom; a top including a base with a downwardly extending wall at least partially bounding the base of the top; a tubular neck with an opening defined therein; and, one or more shelves, wherein, each shelf includes a base with an upwardly extending wall at least partially bounding the base of the shelf. The upwardly extending wall of a first shelf adjoins the downwardly extending wall of the top with the first shelf being located intermediate the bottom and the top. The base of each of the shelves having at least one aperture formed therein. The bottom, the top and the one or more shelves collectively define an enclosed volume for culturing cells. The tubular neck extends from the vessel with the enclosed volume being accessible by the opening in the tubular neck. Advantageously, this vessel provides high volume cell culture in a manner that increases efficiency and reduces the cost of culturing cells.

    摘要翻译： 本发明公开了一种用于培养细胞的容器，其包括：底部，其包括具有至少部分地限定底部的底部的向上延伸壁的基部; 顶部包括具有向下延伸的壁的基部，至少部分地限定顶部的基部; 具有限定在其中的开口的管状颈部; 以及一个或多个搁架，其中每个搁架包括具有向上延伸的壁的基部，至少部分地限定搁架的底部。 第一搁架的向上延伸的壁邻接顶部的向下延伸的壁，第一搁板位于底部和顶部的中间。 每个搁架的底部具有形成在其中的至少一个孔。 底部，顶部和一个或多个搁架共同限定用于培养细胞的封闭体积。 管状颈部从容器延伸，封闭体积可通过管状颈部中的开口接近。 有利地，该容器以提高效率并降低培养细胞成本的方式提供高体积细胞培养。

    Sensor and method for detecting an air borne or exogenously introduced analyte
    8.
    发明授权
    Sensor and method for detecting an air borne or exogenously introduced analyte 有权
    用于检测空气传播或外源引入的分析物的传感器和方法

    公开(公告)号：US06514402B2

    公开(公告)日：2003-02-04

    申请号：US09731423

    申请日：2000-12-06

    申请人： Narayan V. Iyer ,  William J. Lacey ,  David M. Root

    发明人： Narayan V. Iyer ,  William J. Lacey ,  David M. Root

    IPC分类号： G01N2731

    CPC分类号： G01N27/12

    摘要： The present invention includes a method and sensor that is easy to assemble and can operate to effectively detect an air borne or exogenously introduced analyte. In one embodiment, the assembled sensor includes a top cap capable of receiving a first electrolyte and a bottom cap capable of receiving a second electrolyte. The assembled sensor also includes a flexible boot that holds together the top cap, the bottom cap and a membrane. The membrane is located between the first electrolyte and the second electrolyte and enables an electrical device to detect an analyte (e.g., hazardous chemical) which originally entered the sensor through a passage in the top cap and interacted with the membrane.

    摘要翻译： 本发明包括一种方法和传感器，其易于组装并且可以操作以有效地检测空气传播或外源引入的分析物。 在一个实施例中，组装的传感器包括能够接收第一电解质的顶盖和能够接收第二电解质的底盖。 组装的传感器还包括将顶盖，底盖和膜保持在一起的柔性靴。 膜位于第一电解质和第二电解质之间，并且使得电气装置能够检测最初通过顶盖中的通道进入传感器并与膜相互作用的分析物（例如危险化学品）。

    Microplates with UV permeable bottom wells
    9.
    发明授权
    Microplates with UV permeable bottom wells 失效
    具有UV透过底孔的微孔板

    公开(公告)号：US5858309A

    公开(公告)日：1999-01-12

    申请号：US621085

    申请日：1996-03-22

    申请人： Gregory Mathus ,  Paul M. Szlosek ,  William J. Lacey

    发明人： Gregory Mathus ,  Paul M. Szlosek ,  William J. Lacey

    IPC分类号： G01N1/00 ,  B01L3/00 ,  B29C45/14 ,  B29D99/00 ,  B29K105/32 ,  B29L22/00 ,  B29L31/00 ,  B32B27/30 ,  C08L27/12 ,  C12M1/32 ,  G01N1/10 ,  G01N21/03 ,  G01N21/25 ,  G01N21/33

    CPC分类号： B01L3/5085 ,  B29C45/1418 ,  B29C45/14344 ,  B29C45/14778 ,  G01N21/03 ,  G01N21/25 ,  G01N21/33 ,  B01L2300/0829 ,  B01L2300/12

    摘要： Microplates and methods for manufacturing microplates. The microplate is designed to allow UV radiation to pass through the bottom wells of the microplate so that the microplate can be used for assaying samples by use of UV absorbance. In one embodiment, the microplate comprises at least first and second wells, each well having a UV permeable bottom. In another embodiment, the microplate comprises a frame having an upper portion and a lower portion contiguous with the upper portion and a sheet disposed between the upper portion and the lower portion and defining the bottom of at least one well of the microplate. One embodiment of the method includes steps of inserting a sheet of UV permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, injecting molten plastic material into the mold cavity, and cooling the plastic material to form the microplate with the plastic material forming the sidewalls of each of the first and second wells and the sheet of UV permeable material forming the bottom of each of the first and second wells. Another embodiment of the method includes providing an upper plate defining sidewalls of at least one well, adhering an intermediate layer to the upper plate and adhering a sheet of UV permeable material to the intermediate layer. A further embodiment of the method includes inserting a sheet of material having at least one hole into a mold cavity, injecting a molten plastic material into the mold cavity and cooling the plastic material to form a microplate.

    摘要翻译： 微孔板和微孔板制造方法。 微孔板被设计成允许紫外线辐射通过微板的底部孔，使得微孔板可以用于通过使用UV吸光度来测定样品。 在一个实施方案中，微板包括至少第一和第二孔，每个孔具有可UV透过的底部。 在另一个实施例中，微孔板包括具有与上部邻接的上部和下部的框架，以及设置在上部和下部之间并限定微板的至少一个孔的底部的框架。 该方法的一个实施例包括以下步骤：将一片UV可渗透材料插入到模腔中，该模腔包括成形为形成多个孔的侧壁的部分，将熔融的塑料材料注入模腔中，并冷却塑料材料以形成 微板与塑料材料形成第一和第二阱中的每一个的侧壁以及形成第一和第二孔中每一个的底部的UV透过材料片。 该方法的另一实施例包括提供限定至少一个阱的侧壁的上板，将中间层粘附到上板并将一片UV可渗透材料粘合到中间层。 该方法的另一实施例包括将具有至少一个孔的材料片插入模腔中，将熔融的塑料材料注入模腔并冷却塑料以形成微板。

    Tracheostomy mask with tracheostomy tube alignment stabilizer
    10.
    发明授权
    Tracheostomy mask with tracheostomy tube alignment stabilizer 失效
    气管造口术与气管造口管对准稳定器

    公开(公告)号：US5749360A

    公开(公告)日：1998-05-12

    申请号：US794606

    申请日：1997-02-03

    申请人： William J. Lacey ,  John Elkins

    发明人： William J. Lacey ,  John Elkins

    IPC分类号： A61M16/04 ,  A61M16/00 ,  A62B9/06

    CPC分类号： A61M16/047

    摘要： A tracheostomy mask for delivering gaseous therapeutics to a patient fitted with an tracheostomy tube includes a mask body and an alignment stabilizer. The mask body has an opening to receive oxygen and humidification to be delivered to a patient. The alignment stabilizer is supported by the mask body and maintains alignment between the mask body and the tracheostomy tube inserted within the neck of a patient. By maintaining this alignment, the tracheostomy mask with alignment stabilizer insures reliable therapeutic delivery to the patient.

    摘要翻译： 用于将气体治疗剂递送到装配有气管造口管的患者的气管切开面罩包括面罩主体和对准稳定器。 面罩主体具有接收氧气和加湿以将其递送给患者的开口。 对准稳定器由面罩主体支撑并保持面罩主体与插入患者颈部的气管切开管之间的对准。 通过维持这种对准，具有对准稳定剂的气管切开掩模确保了对患者的可靠的治疗递送。