公开(公告)号：US09562895B2

公开(公告)日：2017-02-07

申请号：US12689730

申请日：2010-01-19

申请人： Wayne F. Patton

发明人： Wayne F. Patton

IPC分类号： G01N33/543 ,  C12Q1/42 ,  C12Q1/48 ,  G01N33/542 ,  G01N33/573

CPC分类号： G01N33/54313 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/542 ,  G01N33/54306 ,  G01N33/5432 ,  G01N33/5436 ,  G01N33/573 ,  G01N2333/9121 ,  G01N2333/916

摘要： The invention provides assay particles useful, for example, for detecting analytes and binding molecule interactions. One type of assay particle includes a core portion encased by a shell portion, wherein the shell portion comprises an inorganic phosphor that binds selectively to a target molecule. Another type of an assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the coat portion comprises an inorganic phosphor that binds selectively to a target molecule. A further type of assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the coat portion comprises an inorganic phosphor and a target selective binding moiety, and wherein the assay particle is buoyant in aqueous media. An additional type of assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the shell portion comprises an inorganic phosphor and the coat portion comprises a target selective binding moiety, and wherein the assay particle is buoyant in aqueous media. Also provided are kits and related methods.

摘要翻译： 本发明提供了可用于例如用于检测分析物和结合分子相互作用的测定颗粒。 一种测定颗粒包括由壳部分包围的芯部分，其中壳部分包含与靶分子选择性结合的无机磷光体。 另一类型的测定颗粒包括由壳部分包围的芯部分和覆盖壳部分的涂层部分，其中涂层部分包含与靶分子选择性结合的无机磷光体。 另一类型的测定颗粒包括由壳部分包围的芯部分和覆盖壳部分的涂层部分，其中涂层部分包含无机磷光体和靶选择性结合部分，并且其中测定颗粒在水性介质中是浮力的 。 附加类型的测定颗粒包括由壳部分包围的芯部分和覆盖壳部分的涂层部分，其中壳部分包含无机荧光体，并且涂层部分包含靶选择性结合部分，并且其中测定颗粒为 漂浮在含水介质中。 还提供了套件和相关方法。

公开(公告)号：US07816527B2

公开(公告)日：2010-10-19

申请号：US11182122

申请日：2005-07-15

申请人： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

发明人： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

IPC分类号： C07D239/72

CPC分类号： C07D265/22 ,  C07D239/91 ,  Y10T436/143333 ,  Y10T436/200833

摘要： Kits including novel fluorescent derivitization reagents for labeling an aldehyde- and/or ketone-containing target substance in a sample are described. In one embodiment kits are provided the novel fluorescent derivitization reagents of which have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-16 alkyl, C1-6 alkylamino, amino, or halogen; and R21-R24 are independently H, C 1-6 alkyl, C 1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The kits and reagents described are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.

摘要翻译： 描述了包括用于标记样品中含醛和/或酮的目标物质的新型荧光衍生试剂的试剂盒。 在一个实施方案中提供试剂盒，其新型荧光衍生试剂具有下式：其中Q为羰基，硫代羰基或磺酰基，R5为-L-Z; L是亚芳基或C1-6全氟亚烷基; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R11-R14独立地为H，C1-16烷基，C1-6烷基氨基，氨基或卤素; R 21 -R 24独立地为H，C 1-6烷基，C 1-6烷氧基，C 1-6全氟烷基，C 1-6烷基氨基，二（C 2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基， 硝基，苯基，磺基或-LZ。 所述的试剂盒和试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

公开(公告)号：US20090253156A1

公开(公告)日：2009-10-08

申请号：US11744705

申请日：2007-05-04

申请人： Wayne F. Patton ,  Bing Xie

发明人： Wayne F. Patton ,  Bing Xie

IPC分类号： C12Q1/48 ,  C12Q1/37

CPC分类号： G01N33/573 ,  G01N33/6848 ,  G01N2333/912 ,  G01N2333/916

摘要： The inventions relates to methods and kits for capture and/or analysis of kinases and/or phosphatases in one or more samples. In some embodiments, a kinase inhibitor, e.g. staurosporine or its derivative, is used to capture kinases from a sample. In some embodiments, a phosphatase inhibitor, e.g. microcystin or its derivative, is used to capture phosphatases from a sample. Methods for quantitative analysis of captured kinases and/or proteases are also provided. In some embodiments, quantitative analysis is accomplished using mass spectrometry. In addition, the invention provides kits related to same.

摘要翻译： 本发明涉及用于在一个或多个样品中捕获和/或分析激酶和/或磷酸酶的方法和试剂盒。 在一些实施方案中，激酶抑制剂，例如， 星形孢菌素或其衍生物，用于从样品中捕获激酶。 在一些实施方案中，磷酸酶抑制剂，例如， 微囊藻毒素或其衍生物用于从样品中捕获磷酸酶。 还提供了捕获的激酶和/或蛋白酶的定量分析方法。 在一些实施方案中，使用质谱法进行定量分析。 此外，本发明提供了与其相关的试剂盒。

公开(公告)号：US20080261321A1

公开(公告)日：2008-10-23

申请号：US11571687

申请日：2005-07-06

申请人： Wayne F. Patton ,  Alvydas Mikulskis ,  Eva Golenko

发明人： Wayne F. Patton ,  Alvydas Mikulskis ,  Eva Golenko

IPC分类号： G01N33/00 ,  C07K1/00

CPC分类号： C12Q1/6818 ,  C07K1/22 ,  G01N33/6842 ,  Y10T436/163333 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2563/167

摘要： The invention provides methods for detecting and isolating phosphomolecules using phosphoaffinity materials that comprise a hydrated metal oxide. In an embodiment, a method for detecting a phosphomolecule in a sample involves (a) contacting a sample with a phosphoaffinity material comprising a hydrated metal oxide, under conditions wherein a phosphomolecule is capable of binding to the phosphoaffinity material to form a phosphomolecule-phosphoaffinity material complex, and (b) detecting formation of a phosphomolecule-phosphoaffinity material complex, thereby detecting a phosphomolecule in the sample. In another embodiment, a method for isolating a phosphomolecule from a sample involves (a) contacting a sample with a phosphoaffinity material comprising a hydrated metal oxide, under conditions wherein a phosphomolecule is capable of binding to the phosphoaffinity material to form a phosphomolecule-phosphoaffinity material complex, wherein the hydrated metal oxide comprises yttrium, and (b) separating the phosphomolecule-phosphoaffinity material complex from the sample, thereby isolating the phosphomolecule from the sample.

摘要翻译： 本发明提供了使用包含水合金属氧化物的磷酸亲和物质来检测和分离磷酸分子的方法。 在一个实施方案中，用于检测样品中的磷酸分子的方法包括（a）使样品与包含水合金属氧化物的磷脂质材料接触，条件是其中磷分子能够结合磷酸亲和物质以形成磷酸分子 - 磷酸亲和物质 复合物，和（b）检测磷酸分子 - 磷酸亲和性物质复合物的形成，从而检测样品中的磷酸分子。 在另一个实施方案中，用于从样品中分离磷酸分子的方法包括（a）使样品与包含水合金属氧化物的磷酸亲和材料接触，条件是磷酸分子能够与磷酸亲和性材料结合以形成磷酸分子 - 磷酸亲和物质 复合物，其中水合金属氧化物包含钇，和（b）从样品中分离出磷酸分子 - 磷酸亲和性物质络合物，从而从样品中分离出磷酸分子。

公开(公告)号：US20080182284A1

公开(公告)日：2008-07-31

申请号：US12060834

申请日：2008-04-01

申请人： Richard P. Haugland ,  Karen J. Martin ,  Wayne F. Patton

发明人： Richard P. Haugland ,  Karen J. Martin ,  Wayne F. Patton

IPC分类号： C12Q1/42 ,  G01N33/00 ,  C12Q1/00 ,  G01N33/543 ,  C12Q1/28

CPC分类号： G01N33/54306 ,  C07K1/1077 ,  C07K1/13 ,  G01N27/44726 ,  G01N33/581 ,  G01N33/583 ,  G01N33/6839

摘要： The invention describes methods for labeling or detecting of immobilized poly(amino acids), including peptides, polypeptides and proteins, on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes. Such immobilized poly(amino acids) are labeled or detected on blots or on arrays of poly(amino acids), or are attached to immobilized aptamers.

摘要翻译： 本发明描述了使用荧光二吡咯亚甲基二氟化物染料在膜和其它固体支持物上标记或检测固定的聚（氨基酸），包括肽，多肽和蛋白质的方法。 这种固定的聚（氨基酸）在印迹或聚（氨基酸）阵列上被标记或检测，或连接到固定的适体。

公开(公告)号：US07776533B2

公开(公告)日：2010-08-17

申请号：US11552275

申请日：2006-10-24

申请人： Brian Agnew ,  Joseph Beechem ,  Kyle R Gee ,  Richard P Haugland ,  Thomas H Steinberg ,  Wayne F Patton

发明人： Brian Agnew ,  Joseph Beechem ,  Kyle R Gee ,  Richard P Haugland ,  Thomas H Steinberg ,  Wayne F Patton

IPC分类号： G01N33/53

CPC分类号： G01N33/5008 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709 ,  Y10T436/142222 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/201666

摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

公开(公告)号：US07550271B2

公开(公告)日：2009-06-23

申请号：US11742443

申请日：2007-04-30

申请人： Wayne F. Patton ,  Thomas Edwin Miller

发明人： Wayne F. Patton ,  Thomas Edwin Miller

IPC分类号： C12Q1/48

CPC分类号： C12Q1/485 ,  C12Q1/42

摘要： Disclosed are methods and reagents for detecting the presence of and/or activity of one or more phospho-transfer activities such as kinase, phosphatase, nucleotide cyclase, or phosphodiesterase activities.

摘要翻译： 公开了用于检测一种或多种磷酸转移活性如激酶，磷酸酶，核苷酸环化酶或磷酸二酯酶活性的存在和/或活性的方法和试剂。

公开(公告)号：US06329205B1

公开(公告)日：2001-12-11

申请号：US09652905

申请日：2000-08-30

申请人： Zhenjun Diwu ,  Wayne F. Patton

发明人： Zhenjun Diwu ,  Wayne F. Patton

IPC分类号： G01N2164

CPC分类号： G01N33/6839 ,  G01N1/30 ,  G01N2001/302

摘要： The invention relates to the staining of amine-containing polymers, including including peptides, polypeptides, and proteins, in gels and on solid supports, using complexes of europium (3+).

摘要翻译： 本发明涉及使用铕（3+）的复合物在凝胶和固体支持物上染色含胺聚合物，包括肽，多肽和蛋白质。

公开(公告)号：US08945932B2

公开(公告)日：2015-02-03

申请号：US11571687

申请日：2005-07-06

申请人： Wayne F. Patton ,  Alvydas Mikulskis ,  Eva Golenko

发明人： Wayne F. Patton ,  Alvydas Mikulskis ,  Eva Golenko

IPC分类号： G01N33/52 ,  C12Q1/68 ,  C07K1/22 ,  G01N33/68

CPC分类号： C12Q1/6818 ,  C07K1/22 ,  G01N33/6842 ,  Y10T436/163333 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2563/167

摘要： The invention provides methods for detecting and isolating phosphomolecules using phosphoaffinity materials that comprise a hydrated metal oxide. In an embodiment, a method for detecting a phosphomolecule in a sample involves (a) contacting a sample with a phosphoaffinity material comprising a hydrated metal oxide, under conditions wherein a phosphomolecule is capable of binding to the phosphoaffinity material to form a phosphomolecule-phosphoaffinity material complex, and (b) detecting formation of a phosphomolecule-phosphoaffinity material complex, thereby detecting a phosphomolecule in the sample. In another embodiment, a method for isolating a phosphomolecule from a sample involves (a) contacting a sample with a phosphoaffinity material comprising a hydrated metal oxide, under conditions wherein a phosphomolecule is capable of binding to the phosphoaffinity material to form a phosphomolecule-phosphoaffinity material complex, wherein the hydrated metal oxide comprises yttrium, and (b) separating the phosphomolecule-phosphoaffinity material complex from the sample, thereby isolating the phosphomolecule from the sample.

摘要翻译： 本发明提供了使用包含水合金属氧化物的磷酸亲和物质来检测和分离磷酸分子的方法。 在一个实施方案中，用于检测样品中的磷酸分子的方法包括（a）使样品与包含水合金属氧化物的磷脂质材料接触，条件是其中磷分子能够结合磷酸亲和物质以形成磷酸分子 - 磷酸亲和物质 复合物，和（b）检测磷酸分子 - 磷酸亲和性物质复合物的形成，从而检测样品中的磷酸分子。 在另一个实施方案中，从样品中分离磷酸分子的方法包括（a）使样品与包含水合金属氧化物的磷脂质材料接触，条件是其中磷分子能够结合磷酸亲和物质以形成磷酸分子 - 磷酸亲和物质 复合物，其中所述水合金属氧化物包含钇，和（b）从所述样品中分离所述磷酸分子 - 磷酸亲和性材料配合物，从而从所述样品中分离出所述磷酸分子。

公开(公告)号：US20110117659A1

公开(公告)日：2011-05-19

申请号：US12906014

申请日：2010-10-15

申请人： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

发明人： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

IPC分类号： G01N33/68 ,  G01N33/00

CPC分类号： C07D265/22 ,  C07D239/91 ,  Y10T436/143333 ,  Y10T436/200833

摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.

摘要翻译： 描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。 在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R5是-L-Z; L是亚芳基或C1-6全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R11-R14独立地为H，C1-6烷基，C1-6烷氧基，C1-6全氟烷基，C1-6烷基氨基，二（C2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基，苯基， 或磺基; 和R 21 -R 24独立地为H，C 1-6烷基，C 1-6烷氧基，C 1-6全氟烷基，C 1-6烷基氨基，二（C 2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基， 苯基，磺基或-LZ。 描述了用衍生化试剂处理样品的方法。 这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。