Detection of target nucleic acid using a target hybridization and detection primer
    2.
    发明授权
    Detection of target nucleic acid using a target hybridization and detection primer 有权
    使用靶杂交和检测引物检测靶核酸

    公开(公告)号:US09328377B2

    公开(公告)日:2016-05-03

    申请号:US13508086

    申请日:2009-11-28

    IPC分类号: C12P19/34 C12Q1/68

    摘要: The present invention relates to the detection of a target nucleic acid sequence using a target hybridization and detection primer (THD primer). The present invention allows for both a target amplification and a signal amplification by introducing a label into a primer used in PCR reactions, ensuring a real-time target detection by PCR reaction by no use of complicated oligonucleotides. The present invention could completely be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. This feature makes it possible that the present invention exhibits excellent real-time target detection in multiplex manner.

    摘要翻译: 本发明涉及使用靶杂交和检测引物(THD引物)检测靶核酸序列。 本发明通过在PCR反应中使用的引物中引入标记物来允许目标扩增和信号扩增两者,通过不使用复杂的寡核苷酸来确保PCR反应的实时目标检测。 本发明可以完全免除与常规实时PCR方法相关的麻烦的问题和缺点。 本发明允许仅使用标记的引物成功实现目标检测。 该特征使得本发明可以以多路复用方式显示出优异的实时目标检测。

    DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY PO CLEAVAGE AND HYBRIDIZATION
    4.
    发明申请
    DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY PO CLEAVAGE AND HYBRIDIZATION 有权
    通过PO CLEAVAGE和杂交检测目标核酸序列

    公开(公告)号:US20140073534A1

    公开(公告)日:2014-03-13

    申请号:US14114253

    申请日:2012-05-03

    IPC分类号: C12Q1/68

    摘要: The present invention relates to the detection of a target nucleic acid sequence by a POCH (PO Cleavage and Hybridization) assay on a solid substrate. The present invention detects the target nucleic acid sequence by use of in which the PO (Probing Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved and the cleavage of the PO is detected by hybridization with the CO (Capturing Oligonucleotide). In the present invention, an uncleaved PO is hybridized with the CO immobilized onto the solid substrate. The designs of the PO and the CO are convenient and the optimization of reaction conditions is routinely easy in the present invention. Where the detection of signal on the solid substrate is continuously performed along with repetition of cleavage of the POs in the present invention, the number of the POs cleaved is increased upon the repetition number of the cleavage reaction and the signal is changed in parallel with the number of the POs cleaved. Then, the target nucleic acid sequence can be detected in a real-time manner. In contrast, the change of the signal is not observed in the absence of the target nucleic acid sequence.

    摘要翻译: 本发明涉及通过在固体基质上的POCH(PO切割和杂交)测定法检测靶核酸序列。 本发明通过使用其中与靶核酸序列杂交的PO(探针寡核苷酸)被切割并通过与CO(捕获寡核苷酸)杂交来检测PO的切割来检测靶核酸序列。 在本发明中,未切割的PO与固定在固体基质上的CO杂交。 PO和CO的设计是方便的,并且在本发明中反应条件的优化通常很容易。 在本发明中连续进行PO的切割的情况下连续进行固体基板上的信号检测的情况下,切割反应的次数随着切割反应的重复次数而增加,信号与 PO的数量劈开。 然后,可以以实时的方式检测靶核酸序列。 相比之下,在没有靶核酸序列的情况下,没有观察到信号的变化。

    DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY EXONUCLEOLYTIC ACTIVITY USING SINGLE-LABELED IMMOBILIZED PROBES ON SOLID PHASE
    5.
    发明申请
    DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY EXONUCLEOLYTIC ACTIVITY USING SINGLE-LABELED IMMOBILIZED PROBES ON SOLID PHASE 有权
    通过使用固体相位上的单标记固定化探针检测非离子表面活性剂的目标核酸序列

    公开(公告)号:US20130190208A1

    公开(公告)日:2013-07-25

    申请号:US13824688

    申请日:2010-12-23

    IPC分类号: C12Q1/68

    摘要: The present invention relates to a novel method for detection of target nucleic acid sequences by cyclic exonucleolytic reactions (CER) or exonucleolytic reactions (ER) using single-labeled immobilized probes on a solid phase. The present invention enables to detect target nucleic acid sequences on a solid phase using single-labeled systems. Comparing with multiple-labeled systems such as dual labeling, the present invention using single-labeled probes has excellent advantages in light of convenience and cost effectiveness in probe design and preparation. Furthermore, the measurement of changes of the signal decrease during reactions is responsible for more accurate qualitative and quantitative analysis of target nucleic acid sequences.

    摘要翻译: 本发明涉及通过使用固相上的单标记固定化探针的循环外源核解决反应(CER)或核酸外解反应(ER)检测靶核酸序列的新方法。 本发明能够使用单标记系统检测固相上的靶核酸序列。 与双标记等多标记体系相比,使用单标记探针的本发明在探针设计和制备中的便利性和成本效益方面具有优异的优点。 此外,反应期间信号变化的测量的测量负责更准确地定性和定量分析靶核酸序列。

    Battery management system and driving method thereof
    7.
    发明授权
    Battery management system and driving method thereof 有权
    电池管理系统及其驱动方法

    公开(公告)号:US08111071B2

    公开(公告)日:2012-02-07

    申请号:US11858798

    申请日:2007-09-20

    IPC分类号: G01N27/416 H02J7/00

    摘要: A sensing and control apparatus for a battery management system is provided. The sensing and control apparatus includes: a sensing unit and a main control unit. The sensing unit includes: a cell relay of a plurality of cell relays and a voltage detection unit. The cell relay is configured to be coupled to at least one of a plurality of cells. The voltage detection unit is coupled to a cell relay. The voltage detection unit is configured to: receive a reference voltage when each of the plurality of cell relays is turned off; and generate a second voltage by amplifying by a gain a first voltage that corresponds to the reference voltage. The main control unit is configured to calculate a valid gain corresponding to a ratio of the second voltage to the reference voltage when the temperature of the voltage detection unit is within a threshold temperature range.

    摘要翻译: 提供了一种用于电池管理系统的感测和控制装置。 感测和控制装置包括:感测单元和主控制单元。 感测单元包括:多个单元继电器的单元继电器和电压检测单元。 小区中继被配置为耦合到多个小区中的至少一个小区。 电压检测单元耦合到电池继电器。 电压检测单元被配置为:当多个单电池继电器中的每一个被关闭时,接收参考电压; 并且通过增益放大对应于参考电压的第一电压来产生第二电压。 主控制单元被配置为当电压检测单元的温度在阈值温度范围内时,计算对应于第二电压与参考电压的比率的有效增益。

    Method of transferring and/or receiving data in communication system and apparatus thereof
    8.
    再颁专利
    Method of transferring and/or receiving data in communication system and apparatus thereof 有权
    在通信系统中传送和/或接收数据的方法及其装置

    公开(公告)号:USRE42675E1

    公开(公告)日:2011-09-06

    申请号:US12576005

    申请日:2009-10-08

    IPC分类号: H04B7/216

    摘要: The present invention relates to Walsh Code Allocation (WCA) field in the Packet Data Control Channel. One of the embodiments of the WCA field is use of the WCA field as Last Walsh Code Index (LWCI) field in the message format of the PDCCH. The LWCI field indicates the corresponding last code index or the corresponding number of Walsh codes in a Walsh Code Index (WCI) Table/List/Set. In alternative embodiments, the WCA indicates a general set of Walsh codes or a specific set of Walsh codes in the WCI table. The WCA fields may or may not be used with one of power ranking and sequential decoding, but is preferable used. The WCA field has various advantages including but not limited to efficient use of system resources.

    摘要翻译: 本发明涉及分组数据控制信道中的沃尔什码分配(WCA)字段。 WCA字段的一个实施例是将WCA字段用作PDCCH的消息格式中的最后沃尔什码索引(LWCI)字段。 LWCI字段指示沃尔什代码索引(WCI)表/列表/集合中的相应的最后代码索引或相应的沃尔什码的数量。 在替代实施例中,WCA表示WCI表中的一组沃尔什码或沃尔什码的特定集合。 WCA字段可以使用或不用于功率等级和顺序解码之一,但是优选使用。 WCA领域具有各种优点,包括但不限于有效利用系统资源。

    Method of configuring transmission in mobile communication system
    10.
    发明授权
    Method of configuring transmission in mobile communication system 有权
    在移动通信系统中配置传输的方法

    公开(公告)号:US07712012B2

    公开(公告)日:2010-05-04

    申请号:US11476689

    申请日:2006-06-29

    IPC分类号: H03M13/00 H03M13/03

    摘要: Method for configuring a transmission chain in a 3GPP2 system for supporting a flexible or variable data rate of an information bitstream in a process for mapping an information bitstream of a data rate on a physical layer, including the steps of (1) channel coding the information bitstreams with bit rates different from each other into turbo codes or convolution codes having a value inverse of 1/coding rate, and (2) repeating coded bitstream when the channel coded bitstream is smaller than a desired interleaving size, and puncturing the coded bitstream when the channel coded bitstream is greater than the desired interleaving size, for matching the channel coded bitstream to the interleaving size.

    摘要翻译: 一种用于在3GPP2系统中配置传输链的方法,用于在用于映射物理层上的数据速率的信息比特流的过程中支持信息比特流的灵活或可变数据速率,包括以下步骤:(1)信息编码信息 具有彼此不同的比特率的比特流变成具有1 /编码率的逆值的turbo码或卷积码,以及(2)当信道编码比特流小于期望的交织大小时,重复编码比特流,并且当编码比特流被打破时, 信道编码比特流大于期望的交织大小,用于将信道编码比特流与交织大小相匹配。