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    Polynucleotide barcoding
    1.
    发明申请
    Polynucleotide barcoding 有权
    多核苷酸条形码

    公开(公告)号：US20060094019A1

    公开(公告)日：2006-05-04

    申请号：US10976546

    申请日：2004-10-28

    申请人： Paul Selvin ,  Matthew Gordon ,  Pui-Yan Kwok ,  Ming Xiao ,  Ting-Fung Chan

    发明人： Paul Selvin ,  Matthew Gordon ,  Pui-Yan Kwok ,  Ming Xiao ,  Ting-Fung Chan

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6858 ,  C12Q1/6827

    摘要： A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.

    摘要翻译： 使用这样一种方法对多核苷酸进行条形码化，由此将分离的单个多核苷酸固定在固相上并拉伸，使用靶特异性杂交探针对靶进行标记，并光学检测每个标记靶上的未扩增探针的单个标记。 确定标签的顺序以形成多核苷酸的条形码表示，其中表示靶标及其相对位置。

    Polynucleotide barcoding
    2.
    发明授权
    Polynucleotide barcoding 有权
    多核苷酸条形码

    公开(公告)号：US07829278B2

    公开(公告)日：2010-11-09

    申请号：US10976546

    申请日：2004-10-28

    申请人： Paul R. Selvin ,  Matthew Gordon ,  Pui-Yan Kwok ,  Ming Xiao ,  Ting-Fung Chan

    发明人： Paul R. Selvin ,  Matthew Gordon ,  Pui-Yan Kwok ,  Ming Xiao ,  Ting-Fung Chan

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6858 ,  C12Q1/6827

    摘要： A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.

    摘要翻译： 使用这样一种方法对多核苷酸进行条形码化，由此将分离的单个多核苷酸固定在固相上并拉伸，使用靶特异性杂交探针对靶进行标记，并光学检测每个标记靶上的未扩增探针的单个标记。 确定标签的顺序以形成多核苷酸的条形码表示，其中表示靶标及其相对位置。

    Whole genome mapping by DNA sequencing with linked-paired-end library
    5.
    发明授权
    Whole genome mapping by DNA sequencing with linked-paired-end library 有权

    公开(公告)号：US09758780B2

    公开(公告)日：2017-09-12

    申请号：US14728223

    申请日：2015-06-02

    申请人： Ming Xiao ,  Justin Sibert

    发明人： Ming Xiao ,  Justin Sibert

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  C07H21/02 ,  G06F19/18

    CPC分类号： C12N15/1068 ,  C12Q1/6806 ,  G06F19/18

    摘要： The present invention relates to innovative means of generating sequence-linked DNA fragments and subsequent uses of such linked DNA fragments for de novo haplotype-resolved whole genome mapping and massively parallel sequencing. In various embodiments described herein, the methods of the invention relate to methods of generating paired-end nucleic acid fragment sharing common linker nucleic acid sequences using a nicking endonuclease, a T7 endonuclease, a restriction enzyme, or a transposase, methods of analyzing the nucleotides sequences from the linked-paired-end sequenced fragments, and methods of de novo whole genome mapping. Thus, the methods of this invention allow establishing sequence contiguity across the whole genome, and achieving high-quality, low-cost de novo assembly of complex genomes.

    Whole Genome Mapping by DNA Sequencing With Linked-Paired-End Library
    6.
    发明申请
    Whole Genome Mapping by DNA Sequencing With Linked-Paired-End Library 有权
    通过DNA测序的全基因组映射与链接配对文库

    公开(公告)号：US20150344873A1

    公开(公告)日：2015-12-03

    申请号：US14728223

    申请日：2015-06-02

    申请人： Ming Xiao ,  Justin Sibert

    发明人： Ming Xiao ,  Justin Sibert

    IPC分类号： C12N15/10 ,  G06F19/18 ,  C12Q1/68

    CPC分类号： C12N15/1068 ,  C12Q1/6806 ,  G06F19/18

    摘要： The present invention relates to innovative means of generating sequence-linked DNA fragments and subsequent uses of such linked DNA fragments for de novo haplotype-resolved whole genome mapping and massively parallel sequencing. In various embodiments described herein, the methods of the invention relate to methods of generating paired-end nucleic acid fragment sharing common linker nucleic acid sequences using a nicking endonuclease, a T7 endonuclease, a restriction enzyme, or a transposase, methods of analyzing the nucleotides sequences from the linked-paired-end sequenced fragments, and methods of de novo whole genome mapping. Thus, the methods of this invention allow establishing sequence contiguity across the whole genome, and achieving high-quality, low-cost de novo assembly of complex genomes.

    摘要翻译： 本发明涉及产生序列连接的DNA片段的创新方法以及随后使用这种连接的DNA片段进行从头单体型解析的全基因组测绘和大规模并行测序。 在本文描述的各种实施方案中，本发明的方法涉及使用切口内切核酸酶，T7内切核酸酶，限制酶或转座酶产生分支共同连接体核酸序列的配对末端核酸片段的方法，分析核苷酸的方法 来自连锁配对末端测序片段的序列，以及从头整体基因组测绘的方法。 因此，本发明的方法允许跨整个基因组建立序列连续性，并实现复杂基因组的高品质，低成本的从头装配。

    Compact spectrofluorometer
    8.
    发明授权
    Compact spectrofluorometer 有权
    紧凑型荧光分光光度计

    公开(公告)号：US06323944B1

    公开(公告)日：2001-11-27

    申请号：US09443392

    申请日：1999-11-19

    申请人： Ming Xiao

    发明人： Ming Xiao

    IPC分类号： G01N2164

    CPC分类号： G01J3/4406 ,  G01J3/02 ,  G01J3/0256 ,  G01J3/26 ,  G01J3/2803 ,  G01J2003/1213 ,  G01N21/645

    摘要： Spectrofluorometer employing a pair of linear variable spectral filters to produce a three dimensional data output is disclosed. A collimated white light source is used that first passes through a first linear variable spectral filter, then through a sample where fluorescence occurs, then the resultant light passes through a second linear variable spectral light filter that is oriented at ninety degrees from the first filter. The light is then detected by a CCD sensor for conversion into data. This arrangement provides a very simple, rugged and compact instrument that can be used almost anywhere, such as at the scene of a contamination accident.

    摘要翻译： 公开了使用一对线性可变光谱滤波器产生三维数据输出的分光荧光计。 使用准直的白光源，其首先通过第一线性可变光谱滤光器，然后通过荧光发生的样品，然后所得光通过从第一滤光器定向成90度的第二线性可变光谱滤光器。 然后由CCD传感器检测光转换成数据。 这种安排提供了一种非常简单，坚固耐用的紧凑型仪器，可以在几乎任何地方使用，例如在污染事故现场。

    POWER SCHOTTKY BARRIER DIODES WITH HIGH BREAKDOWN VOLTAGE AND LOW LEAKAGE CURRENT
    9.
    发明申请
    POWER SCHOTTKY BARRIER DIODES WITH HIGH BREAKDOWN VOLTAGE AND LOW LEAKAGE CURRENT 有权

    公开(公告)号：US20220352390A1

    公开(公告)日：2022-11-03

    申请号：US17244394

    申请日：2021-04-29

    申请人： Yuhao ZHANG ,  Ming Xiao

    发明人： Yuhao ZHANG ,  Ming Xiao

    IPC分类号： H01L29/872 ,  H01L29/20 ,  H01L29/205 ,  H01L29/06 ,  H01L29/417 ,  H01L29/66

    摘要： This disclosure provides a diode including a semiconductor region having at least one two-dimensional carrier channel of a first conductivity type. The diode also includes a plurality of sidewalls exposed in the semiconductor region defining at least one trench extending through the at least one two-dimensional carrier channel and a material of a second conductivity type, the second conductivity type being the other of the n-type and the p-type conductivity, disposed on the plurality of sidewalls and in contact with the at least one two-dimensional carrier channel. The diode also includes an anode material in contact with at least a portion of the semiconductor region and in contact with at least a portion of the material of the second conductivity type, and a cathode material in contact with the at least one two-dimensional carrier channel.

    Mini scented wax heating device
    10.
    发明申请
    Mini scented wax heating device 审中-公开
    迷你香味蜡加热装置

    公开(公告)号：US20060163240A1

    公开(公告)日：2006-07-27

    申请号：US11042727

    申请日：2005-01-24

    申请人： Ming Xiao

    发明人： Ming Xiao

    IPC分类号： F27D11/00

    CPC分类号： A61L9/03

    摘要： A mini scented wax heating device includes a heating cylinder which is in a penetrating cylindrical form and installs a heating device therein. The heating device comprises a heat plate and a ceramic heating piece attached to the heat plate, and the heat plate is latched to a fixed end of the heating cylinder. The invention uses the heat plate to directly heat the bottom of the heating device. Since the heating device is a mini heating device that heats the heat plate directly by the ceramic heating piece, the heat plate can directly heat and vaporize a piece of scented wax. The invention increases the heating area of the scented wax by the heater, shortens the heating time, and evenly heats the scented wax in the heater, and thus can achieve a better vaporization effect. The invention features a simple structure, a low cost, and a powerful function.

    摘要翻译： 迷你香味蜡加热装置包括加热缸，该加热缸呈贯穿圆柱形并在其中安装加热装置。 加热装置包括加热板和附接到加热板的陶瓷加热片，并且加热板被锁定到加热缸的固定端。 本发明使用加热板直接加热加热装置的底部。 由于加热装置是通过陶瓷加热片直接加热加热板的微型加热装置，所以加热板可以直接加热和蒸发一块香味的蜡。 本发明通过加热器增加了香味蜡的加热面积，缩短了加热时间，并且均匀地加热加热器中的香味蜡，从而可以获得更好的蒸发效果。 本发明具有结构简单，成本低，功能强大的特点。