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    MUTATIONS THAT CONFER GENETIC STABILITY TO ADDITIONAL GENES IN INFLUENZA VIRUSES
    1.
    发明申请
    MUTATIONS THAT CONFER GENETIC STABILITY TO ADDITIONAL GENES IN INFLUENZA VIRUSES 审中-公开

    公开(公告)号：US20200237899A1

    公开(公告)日：2020-07-30

    申请号：US16749910

    申请日：2020-01-22

    申请人： Yoshihiro Kawaoka ,  Yuri Furusawa

    发明人： Yoshihiro Kawaoka ,  Yuri Furusawa

    IPC分类号： A61K39/145 ,  C12N7/00 ,  A61P31/16

    摘要： The disclosure provides for an isolated recombinant influenza virus having at least one of: a PB2 viral segment encoding PB2 with residue at position 540 that is not asparagine or a residue at position 712 that is not glutamic acid, a PA viral segment encoding PA with a residue at position 180 that is not glutamine or a residue at position 200 that is not threonine, or a PB1 viral segment encoding PB1 with a residue at position 149 that is not valine, a residue at position 684 that is not glutamic acid or a residue at position 685 that is not aspartic acid, or any combination thereof, and methods of making and using the virus.

    CELL-BASED SYSTEMS FOR PRODUCING INFLUENZA VACCINES
    6.
    发明申请
    CELL-BASED SYSTEMS FOR PRODUCING INFLUENZA VACCINES 审中-公开
    用于生产流感疫苗的基于细胞的系统

    公开(公告)号：US20110150925A1

    公开(公告)日：2011-06-23

    申请号：US12997131

    申请日：2009-06-09

    申请人： Pamuk Bilsel ,  Yoshihiro Kawaoka ,  Gabriele Neumann

    发明人： Pamuk Bilsel ,  Yoshihiro Kawaoka ,  Gabriele Neumann

    IPC分类号： A61K39/145 ,  C12N7/00 ,  C12N5/10 ,  A61P31/16

    CPC分类号： A61K39/145 ,  A61K39/12 ,  C12N7/00 ,  C12N2760/16034 ,  C12N2760/16051 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12Y204/99007

    摘要： The present invention relates to a cell-based method for producing influenza virus vaccines by enriching the population of surface-bound α2,6-sialic acid receptors on a cell surface, such as on a Chinese Hamster Ovary (CHO) cell surface. The host cell therefore presents numerous binding sites to which an influenza virus can bind via its hemagglutinin spike protein and infect the host cell. In contrast to wild-type CHO cells, the surface of the mutated CHO cells of the present invention contains an enriched population of α2,6-sialic acid receptors which makes the inventive CHO cells highly susceptible to viral infection, and therefore safe, effective, and highly efficient cells for rapidly producing influenza vaccines.

    摘要翻译： 本发明涉及通过富集细胞表面如中国仓鼠卵巢（CHO）细胞表面上的表面结合的α2,6-唾液酸受体群体来生产流感病毒疫苗的基于细胞的方法。 因此，宿主细胞呈现许多结合位点，流感病毒可以通过其血凝素尖峰蛋白结合并感染宿主细胞。 与野生型CHO细胞相比，本发明的突变的CHO细胞的表面含有丰富的α2,6-唾液酸受体群体，这使得本发明的CHO细胞对病毒感染高度敏感，因此是安全，有效的， 以及用于快速生产流感疫苗的高效细胞。

    RECOMBINANT INFLUENZA VIRUSES FOR VACCINES AND GENE THERAPY
    7.
    发明申请
    RECOMBINANT INFLUENZA VIRUSES FOR VACCINES AND GENE THERAPY 审中-公开
    用于疫苗和基因治疗的重组流感病毒

    公开(公告)号：US20090246830A1

    公开(公告)日：2009-10-01

    申请号：US12349769

    申请日：2009-01-07

    申请人： Yoshihiro KAWAOKA ,  Gabriele Neumann

    发明人： Yoshihiro KAWAOKA ,  Gabriele Neumann

    IPC分类号： C12P21/02

    CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  A61K48/00 ,  A61K48/0091 ,  A61K2039/525 ,  A61K2039/5258 ,  C07K14/005 ,  C12N15/85 ,  C12N15/86 ,  C12N2760/16121 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16143 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2800/30 ,  C12N2810/6081

    摘要： A method for producing influenza virus particles for preparation of a vaccine. The method comprises growing reassortant influenza viruses to produce virus particles and recovering the produced virus particles. The reassortant influenza viruses are produced in cultured cells in the absence of helper virus by introducing into the cultured cells expression vectors sufficient to produce genomic vRNA or antigenomic vRNA (cRNA) segments in the cells of reassortant influenza viruses. The method produces a nucleoprotein and an RNA dependent RNA polymerase in the cells so that RNP complexes containing the genomic vRNA segments of the reassortant influenza viruses are formed. The method uses expression vectors containing HA, NA, non-HA and non-NA vRNA segments in which the HA and NA vRNA segments are HA and NA vRNA segments of a viral strain different from a strain that contains all of the non-HA and non-NA vRNA segments.

    摘要翻译： 用于制备疫苗的流感病毒颗粒的制备方法。 该方法包括生长重配流感病毒以产生病毒颗粒并回收产生的病毒颗粒。 在不存在辅助病毒的培养细胞中通过将足够产生重组流感病毒细胞中的基因组vRNA或抗原基团vRNA（cRNA）区段的表达载体导入培养细胞中来产生重配流感病毒。 该方法在细胞中产生核蛋白和RNA依赖性RNA聚合酶，从而形成含有重配流感病毒的基因组vRNA片段的RNP复合物。 该方法使用包含HA，NA，非HA和非NAVRNA片段的表达载体，其中HA和NAvRNA片段是不同于包含所有非HA的菌株的病毒株的HA和NA vRNA片段，以及 非NA vRNA片段。