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公开(公告)号：US4920097A
公开(公告)日：1990-04-24
申请号：US256581
申请日：1988-10-12
CPC分类号： A61K35/15 , Y10S530/829
摘要： Methods of extracting an immunosuppressor from leukocyte dialysates are disclosed, together with methods for using the immunosuppressor and compositions containing the immunosuppressor.
摘要翻译： 公开了从白细胞透析液中提取免疫抑制剂的方法，以及使用免疫抑制剂的方法和含有免疫抑制剂的组合物。
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公开(公告)号：US07432362B2
公开(公告)日：2008-10-07
申请号：US11431030
申请日：2006-05-10
申请人： Sudhir K. Sinha , Dale J. Hedges , Mark A. Batzer
发明人： Sudhir K. Sinha , Dale J. Hedges , Mark A. Batzer
IPC分类号： C07H21/02
CPC分类号： C12Q1/6876 , C12Q1/6879 , C12Q2600/156 , Y10S435/975
摘要： A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the possibility of error to a negligible quantity. The inserted chromosome yields a large fragment when the homologous region is amplified. The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction regents.
摘要翻译： 一种从人类DNA样本中确定性别的方法。 根据片段的大小选择，扩增和评估Alu元件插入的位点。 性别测定使用AluSTXa作为X染色体，AluSTYa用于Y染色体，或同时使用AluSTXa和AluSTYa，以将错误的可能性降低到可忽略的数量。 当扩增同源区时，插入的染色体产生大片段。 男性的区别在于存在两个DNA扩增子，而女性只有一个扩增子。 用于实施该方法的试剂盒包括一对扩增基因座和任选的聚合酶链反应试剂的引物。
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公开(公告)号：US07794983B2
公开(公告)日：2010-09-14
申请号：US11826020
申请日：2007-07-11
申请人： Sudhir K. Sinha , Anthony B. Carter
发明人： Sudhir K. Sinha , Anthony B. Carter
CPC分类号： C12Q1/6858 , C12Q2535/125
摘要： The way to design a “filled” site (which contains an interspersed element) primer set to target a particular locus is to design one of the two primers such that it encompasses that unique information (e.g., interspersed element+flanking genomic sequence+direct repeat). The way to design an “empty” site primer is to design one of the two primers such that the entire direct repeat sequence in addition to flanking genomic sequence is included on both sides. To improve efficiency, the “empty” site primer designed around the direct repeat should not be too long. This primer design of the present invention allows for the ability to test any type of interspersed genetic element containing characteristic direct repeat sequences (direct repeats). This gives the option of many new polymorphic marker sites because Alu elements are not the only interspersed genetic elements having direct repeats flanking their core sequence.
摘要翻译： 设计一个针对特定位点的“填充”位点（其包含散置元素）引物集的方法是设计两个引物之一，使得其包含唯一信息（例如，散在的元件+侧翼基因组序列+直接重复 ）。 设计“空”位点引物的方法是设计两个引物之一，使得除了侧翼基因组序列之外的整个直接重复序列都包含在两侧。 为了提高效率，围绕直接重复设计的“空”站点引导不应该太长。 本发明的该引物设计允许测试含有特征直接重复序列（直接重复）的任何类型的散在遗传元件的能力。 这给出了许多新的多态性标记位点的选择，因为Alu元件不是在核心序列侧面具有直接重复的唯一散布的遗传元件。
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公开(公告)号：US20080206755A1
公开(公告)日：2008-08-28
申请号：US11826020
申请日：2007-07-11
申请人： Sudhir K. Sinha , Anthony B. Carter
发明人： Sudhir K. Sinha , Anthony B. Carter
CPC分类号： C12Q1/6858 , C12Q2535/125
摘要： The way to design a “filled” site (which contains an interspersed element) primer set to target a particular locus is to design one of the two primers such that it encompasses that unique information (e.g., interspersed element+flanking genomic sequence+direct repeat). The way to design an “empty” site primer is to design one of the two primers such that the entire direct repeat sequence in addition to flanking genomic sequence is included on both sides. To improve efficiency, the “empty” site primer designed around the direct repeat should not be too long. This primer design of the present invention allows for the ability to test any type of interspersed genetic element containing characteristic direct repeat sequences (direct repeats). This gives the option of many new polymorphic marker sites because Alu elements are not the only interspersed genetic elements having direct repeats flanking their core sequence.
摘要翻译： 设计一个针对特定位点的“填充”位点（其包含散置元素）引物集的方法是设计两个引物之一，使得其包含唯一信息（例如，散在的元件+侧翼基因组序列+直接重复 ）。 设计“空”位点引物的方法是设计两个引物之一，使得除了侧翼基因组序列之外的整个直接重复序列都包含在两侧。 为了提高效率，围绕直接重复设计的“空”站点引导不应该太长。 本发明的该引物设计允许测试含有特征直接重复序列（直接重复）的任何类型的散在遗传元件的能力。 这给出了许多新的多态性标记位点的选择，因为Alu元件不是在核心序列侧面具有直接重复的唯一散布的遗传元件。
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公开(公告)号：US20100025244A1
公开(公告)日：2010-02-04
申请号：US12458422
申请日：2009-07-10
IPC分类号： G01N33/559 , C12Q1/68
CPC分类号： C07H21/04 , C12Q1/6888
摘要： A family of PCR assays is disclosed for determining, both qualitatively and quantitatively, presence of material from a predetermined species source and for quantifying the amount of such material. The assays are based respectively on SINEs uniquely characteristic of pig species, cow species, chicken species, and ruminant sub-order, and having a high copy number. The assays disclosed permit rapid, inexpensive evaluation of meat samples to facilitate elimination from their diet of pork or beef by persons desiring to avoid such food sources; as well as the assay of cattle feed to determine presence therein of ruminant-source proteins, which are a potential source of bovine spongiform encephalopathy (BSE), commonly referred to as “mad cow disease.” The assays amplify the predetermined unique SINEs and the resulting amplified mixture is then evaluated qualitatively by electrophoresis on gel containing ethidium bromide or quantitatively by SYBR Green-based detection or TaqMan chemistry. The invention also extends to kits, primers, and other products used in connection with the assays. The amplicons are selected to be from about 100 to 170 bp long.
摘要翻译： 公开了一系列PCR测定法，用于从定性和定量上确定来自预定物种来源的材料的存在和用于量化此类材料的量。 测定分别基于猪种，牛种，鸡种和反刍动物次级的独特特征的SINE，并具有高拷贝数。 所公开的测定允许对肉类样品进行快速，便宜的评估，以便于希望避免这种食物来源的人排除猪肉或牛肉的饮食; 以及牛饲料的测定以确定其中的反刍动物源蛋白质，其是牛海绵状脑病（BSE）的潜在来源，通常被称为“疯牛病”。 测定法扩增预定的独特SINE，然后通过在含有溴化乙锭的凝胶上电泳定性地定量得到所得扩增的混合物，或定量地通过SYBR Green检测或TaqMan化学。 本发明还扩展到试剂盒，引物和与测定结合使用的其它产品。 扩增子选择为约100至170bp长。
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公开(公告)号：US07537889B2
公开(公告)日：2009-05-26
申请号：US10673575
申请日：2003-09-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6858 , C12Q2531/113 , C12Q2525/151
摘要： An assay for determining presence of human DNA in a sample in which non-human DNA may also be present and for quantitating such human DNA. One embodiment uses intra-Alu based PCR and another uses inter-Alu based PCR. The assays are performed without unique, expensive equipment. The assays are based on detection of multiple-copy Alu elements recently integrated into the human genome that are largely absent from non-human primates and other mammals.
摘要翻译： 用于确定样品中人类DNA存在的测定法，其中也可以存在非人类DNA，并用于定量这样的人类DNA。 一个实施方案使用基于Alu的PCR，另一个实施方案使用基于Alu的PCR。 无需独特且昂贵的设备进行测定。 该测定基于最近整合到人类基因组中的多拷贝Alu元件的检测，其大部分不存在于非人灵长类动物和其他哺乳动物中。
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公开(公告)号：US07927841B2
公开(公告)日：2011-04-19
申请号：US12458422
申请日：2009-07-10
CPC分类号： C07H21/04 , C12Q1/6888
摘要： A family of PCR assays is disclosed for determining, both qualitatively and quantitatively, presence of material from a predetermined species source and for quantifying the amount of such material. The assays are based respectively on SINEs uniquely characteristic of pig species, cow species, chicken species, and ruminant sub-order, and having a high copy number. The assays disclosed permit rapid, inexpensive evaluation of meat samples to facilitate elimination from their diet of pork or beef by persons desiring to avoid such food sources; as well as the assay of cattle feed to determine presence therein of ruminant-source proteins, which are a potential source of bovine spongiform encephalopathy (BSE), commonly referred to as “mad cow disease.” The assays amplify the predetermined unique SINEs and the resulting amplified mixture is then evaluated qualitatively by electrophoresis on gel containing ethidium bromide or quantitatively by SYBR Green-based detection or TaqMan chemistry. The invention also extends to kits, primers, and other products used in connection with the assays. The amplicons are selected to be from about 100 to 170 bp long.
摘要翻译： 公开了一系列PCR测定法，用于确定来自预定物种来源的材料的定性和定量存在以及用于量化此类材料的量。 测定分别基于猪种，牛种，鸡种和反刍动物次级的独特特征的SINE，并具有高拷贝数。 所公开的测定允许对肉类样品进行快速，便宜的评估，以便于希望避免这种食物来源的人排除猪肉或牛肉的饮食; 以及牛饲料的测定以确定其中的反刍动物源蛋白质，其是牛海绵状脑病（BSE）的潜在来源，通常称为“疯牛病”。该测定扩增了预定的独特SINE和 然后通过在含有溴化乙锭的凝胶上电泳定性地评估所得扩增的混合物，或者通过SYBR Green检测或TaqMan化学定量评价。 本发明还扩展到试剂盒，引物和与测定结合使用的其它产品。 扩增子选择为约100至170bp长。
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公开(公告)号：US07074567B2
公开(公告)日：2006-07-11
申请号：US10673854
申请日：2003-09-30
申请人： Sudhir K. Sinha , Dale J. Hedges , Mark A. Batzer
发明人： Sudhir K. Sinha , Dale J. Hedges , Mark A. Batzer
CPC分类号： C12Q1/6879 , C12Q1/6876 , C12Q2600/156
摘要： A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the possibility of error to a negligible quantity. The inserted chromosome yields a large fragment when the homologous region is amplified. The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction regents.
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公开(公告)号：US07582452B2
公开(公告)日：2009-09-01
申请号：US10736912
申请日：2003-12-17
CPC分类号： C07H21/04 , C12Q1/6888
摘要： A family of PCR assays is disclosed for determining, both qualitatively and quantitatively, presence of material from a predetermined species source and for quantifying the amount of such material. The assays are based respectively on SINEs uniquely characteristic of pig species, cow species, chicken species, and ruminant sub-order, and having a high copy number. The assays disclosed permit rapid, inexpensive evaluation of meat samples to facilitate elimination from their diet of pork or beef by persons desiring to avoid such food sources; as well as the assay of cattle feed to determine presence therein of ruminant-source proteins, which are a potential source of bovine spongiform encephalopathy (BSE), commonly referred to as “mad cow disease.” The assays amplify the predetermined unique SINEs and the resulting amplified mixture is then evaluated qualitatively by electrophoresis on gel containing ethidium bromide or quantitatively by SYBR Green-based detection or TaqMan chemistry. The invention also extends to kits, primers, and other products used in connection with the assays. The amplicons are selected to be from about 100 to 170 bp long.
摘要翻译： 公开了一系列PCR测定法，用于确定来自预定物种来源的材料的定性和定量存在以及用于量化此类材料的量。 测定分别基于猪种，牛种，鸡种和反刍动物次级的独特特征的SINE，并具有高拷贝数。 所公开的测定允许对肉类样品进行快速，便宜的评估，以便于希望避免这种食物来源的人排除猪肉或牛肉的饮食; 以及牛饲料的测定以确定其中的反刍动物源蛋白质，其是牛海绵状脑病（BSE）的潜在来源，通常被称为“疯牛病”。 测定法扩增预定的独特SINE，然后通过在含有溴化乙锭的凝胶上电泳定性地定量得到所得扩增的混合物，或定量地通过SYBR Green检测或TaqMan化学。 本发明还扩展到试剂盒，引物和与测定结合使用的其它产品。 扩增子选择为约100至170bp长。
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10.发明授权Multiplex PCR for simultaneous quantitation of human nuclear, mitochondrial, and male Y-chromosome DNA 有权用于同时定量人类核，线粒体和雄性Y染色体DNA的多重PCR公开(公告)号：US07405044B2
公开(公告)日：2008-07-29
申请号：US11245444
申请日：2005-10-07
CPC分类号： C12Q1/6879 , C12Q1/6851 , C12Q1/6888 , C12Q2600/16 , C12Q2537/143
摘要： A comprehensive set of human specific, target specific, multiplex PCR assays for DNA quantitation is provided. Our duplex qPCR for nDNA/mtDNA had a linear quantitation range of 100 ng to 1 pg, and our triplex qPCR assay for nDNA/mtDNA/male Y DNA had a linear range of 100 ng to 0.1 ng. Human-specificity was demonstrated by the accurate detection of 0.05% and 5% human DNA, respectively, from a complex source of starting templates. Target-specificity was confirmed by the lack of cross-amplification among targets. A high throughput alternative for human gender determination was also developed by multiplexing the male Y primer/probe set with an X chromosome based system. Background cross-amplification with DNA templates derived from fourteen other species was negligible aside from the male Y assay which produced spurious amplifications from other non-human primate templates. Mainstream application of these assays will undoubtedly benefit forensic genomics.
摘要翻译： 提供了一套全面的人类特异性，靶特异性，多重PCR测定用于DNA定量。 我们用于nDNA / mtDNA的双重qPCR线性定量范围为100 ng至1 pg，我们对nDNA / mtDNA /雄性Y DNA的三重qPCR检测线性范围为100 ng至0.1 ng。 通过分别从起始模板的复杂来源准确检测0.05％和5％的人类DNA，证明了人的特异性。 目标特异性由目标缺乏交叉扩增证实。 通过将Y型引物/探针组与基于X染色体的系统进行复用也开发了用于人类性别测定的高通量替代物。 来自十四种其他物种的DNA模板的背景交叉扩增除了从其他非人灵长类动物模板产生杂种扩增的雄性Y测定之外，可忽略不计。 这些测定的主流应用无疑将有益于法医基因组学。
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