公开(公告)号：US20230204508A1

公开(公告)日：2023-06-29

申请号：US18084997

申请日：2022-12-20

Applicant: ACADEMIA SINICA

Inventor： Pei-Kuen WEI ,  Sheng-Hann WANG ,  Ting-Wei CHANG

IPC: G01N21/552

CPC classification number: G01N21/554 ,  G01N2021/177

Abstract: A detection method and a detection system for detecting objects of interest attached to a surface of a plurality of reporters, wherein the plurality of reporters are flowing in a microfluidic chip and illuminated by a light source. The detection method has following steps: obtaining a plurality of local surface plasmon resonance (LSPR) spectral images of each the plurality of the reporters individually, wherein each of the LSPR spectral images has a brightness of a long wavelength band (BA) and a brightness of a short wavelength band (BB); calculating a spectral image brightness contrast γ for each of the LSPR spectral images, wherein





γ
=



B
A

-

B
B




B
A

+

B
B




;




and, defining a positive threshold for |γ|≥0.1.

公开(公告)号：US20240248033A1

公开(公告)日：2024-07-25

申请号：US18417471

申请日：2024-01-19

Applicant: Academia Sinica

Inventor： Chia-Fu CHOU ,  Pei-Kuen WEI ,  Liang-Kun YU ,  Deng-Kai YANG ,  Jui-Hong WENG ,  Kuang-Li LEE ,  Shu-Cheng LO

IPC: G01N21/552 ,  B82Y15/00 ,  G01N21/19 ,  G01N21/25

CPC classification number: G01N21/554 ,  B82Y15/00 ,  G01N21/19 ,  G01N2021/258 ,  G01N2201/0633

Abstract: A surface plasmon resonance sensor is provided, which comprises: a substrate; an adaptation layer disposed on the substrate and comprising a dielectric material; and a metal layer disposed on the adaptation layer, wherein the metal layer has a grating structure comprising plural metal lines. Furthermore, a surface plasmon resonance sensing instrument comprising the same and a method for detecting an analyte using the same are also provided.

公开(公告)号：US20220011228A1

公开(公告)日：2022-01-13

申请号：US17282163

申请日：2019-10-03

Applicant: ACADEMIA SINICA

Inventor： Pei-Kuen WEI ,  Ming-Yang PAN ,  Kuang-Li LEE

IPC: G01N21/552

Abstract: A method and apparatus for surface plasmon resonance imaging are provided for imaging the surface plasmon resonance signals caused by the interaction of biomolecules. In particular, the method and apparatus can image the slightly spectral change in a surface plasmon resonance mode by comparing the light intensities of two bands in the frequency domain of the surface plasmon.

公开(公告)号：US20210025009A1

公开(公告)日：2021-01-28

申请号：US16940284

申请日：2020-07-27

Applicant: TAIPEI MEDICAL UNIVERSITY ,  Academia Sinica

Inventor： Yueh-Lin WU ,  Hsi-Hsien CHEN ,  Heng LIN ,  Ji-Yen CHENG ,  Pei-Kuen WEI

IPC: C12Q1/6886 ,  C12Q1/686

Abstract: The present invention pertains to detection of a tumor in a urinary organ using microRNA(s). MiR-200c-5p, miR-214-3p, miR-21-5p, miR-21-3p, miR-210-3p and/or miR-200b-3p can be used as biomarkers of tumors in a urinary organ to detect a tumor in or predict a prognosis of a tumor in a urinary organ.

公开(公告)号：US20170299788A1

公开(公告)日：2017-10-19

申请号：US15488050

申请日：2017-04-14

Applicant: ACADEMIA SINICA

Inventor： Pei-Kuen WEI ,  En-Hung LIN ,  Shu-Cheng LO ,  Kuang-Li LEE

IPC: G02B5/18 ,  G01J3/02 ,  G01J3/18

CPC classification number: G02B5/1861 ,  G01J3/0221 ,  G01J3/24 ,  G01J2003/1852 ,  G01J2003/1857 ,  G02B5/1852

Abstract: A curved diffraction grating includes a substrate and a metal layer. The substrate is a two-dimensional curved plate structure and has a first surface, a second surface and a plurality of microstructures. The first surface is disposed opposite to the second surface, and the microstructures are disposed on the second surface. Each of the microstructures is a saw-tooth structure and has a clear blazed angle. The metal layer is disposed on the microstructures and has a plurality of diffraction structures corresponding to the microstructures. A spectrometer containing the curved diffraction grating and a manufacturing method of the curved diffraction grating are also disclosed.

公开(公告)号：US20150080253A1

公开(公告)日：2015-03-19

申请号：US14476191

申请日：2014-09-03

Applicant: Academia Sinica

Inventor： Pei-Kuen WEI ,  Kuang-Li LEE

IPC: G01N21/59 ,  G01N21/21

CPC classification number: G01N21/59 ,  G01N2021/5903

Abstract: The present invention provides a label-free sensing chip for identifying a chemical substance, comprising: (a) a transparent substrate comprising a base and first periodic ridges; and (b) a metal layer covering said transparent substrate, comprising second periodic ridges and third periodic ridges, in which said second periodic ridges has a height equal to or greater than the height of the first periodic ridges, and each ridge of the second periodic ridges fits into the space between each ridge of the first periodic ridges, and said third periodic ridges correspondingly located on said first periodic ridges. The present invention also provides a method for identifying a chemical substance by using the foresaid label-free sensing chip.

Abstract translation: 本发明提供了一种用于识别化学物质的无标签感测芯片，包括：（a）包括基底和第一周期性脊的透明基底; 和（b）覆盖所述透明衬底的金属层，包括第二周期性脊和第三周期性脊，其中所述第二周期性脊具有等于或大于第一周期性脊的高度的高度，以及第二周期性脊的每个脊 脊部装配到第一周期性脊脊的每个脊之间的空间中，并且所述第三周期性脊相应地位于所述第一周期性脊上。 本发明还提供了一种通过使用上述无标记感测芯片来鉴别化学物质的方法。