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公开(公告)号：US20130260425A1
公开(公告)日：2013-10-03
申请号：US13912313
申请日：2013-06-07
Applicant: Ajinomoto Co., Inc.
Inventor： Hidetaka Doi , Yasushi Hoshino , Yuri Masumitsu , Yoshihiro Usuda
CPC classification number: C12P13/08 , C07K14/245 , C12N1/20 , C12N1/38 , C12N15/746 , C12P13/04
Abstract: A method for producing an L-amino acid includes culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium. A bacterium which has been subjected to a modification including at least one of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof, is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.
Abstract translation: L-氨基酸的制造方法包括在含有选自脂肪酸和醇的碳源的培养基中培养属于肠杆菌科的细菌并具有L-氨基酸生产能力的细菌， 来自培养基的氨基酸。 使用已经进行了包括oxyS基因表达增强，固定化ABC基因表达的增强及其组合中的至少一种的修饰的细菌作为细菌，或者添加减少细菌的细胞内过氧化氢浓度的物质 到中等
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公开(公告)号：US08951760B2
公开(公告)日：2015-02-10
申请号：US13912313
申请日：2013-06-07
Applicant: Ajinomoto Co., Inc.
Inventor： Hidetaka Doi , Yasushi Hoshino , Yuri Masumitsu , Yoshihiro Usuda
CPC classification number: C12P13/08 , C07K14/245 , C12N1/20 , C12N1/38 , C12N15/746 , C12P13/04
Abstract: A method for producing an L-amino acid includes culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium. A bacterium which has been subjected to a modification including at least one of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof, is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.
Abstract translation: L-氨基酸的制造方法包括在含有选自脂肪酸和醇的碳源的培养基中培养属于肠杆菌科的细菌并具有L-氨基酸生产能力的细菌， 来自培养基的氨基酸。 使用已经进行了包括oxyS基因表达增强，固定化ABC基因表达的增强及其组合中的至少一种的修饰的细菌作为细菌，或者添加减少细菌的细胞内过氧化氢浓度的物质 到中等
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公开(公告)号：US10563234B2
公开(公告)日：2020-02-18
申请号：US15361645
申请日：2016-11-28
Applicant: AJINOMOTO CO., INC.
Inventor： Hidetaka Doi , Akiko Matsudaira , Yoshihiro Usuda
Abstract: A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, wherein the bacterium has been modified so that the activity of aconitase is increased, or the activities of aconitase and acetaldehyde dehydrogenase are increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.
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公开(公告)号：US20170073714A1
公开(公告)日：2017-03-16
申请号：US15361645
申请日：2016-11-28
Applicant: AJINOMOTO CO., INC.
Inventor： Hidetaka Doi , Akiko Matsudaira , Yoshihiro Usuda
IPC: C12P13/08
Abstract: A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, wherein the bacterium has been modified so that the activity of aconitase is increased, or the activities of aconitase and acetaldehyde dehydrogenase are increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.
Abstract translation: 提供了L-氨基酸的制造方法。 通过培养属于肠杆菌科的细菌并具有L-氨基酸生产能力来生产L-氨基酸，其中细菌已被修饰，使得乌头酸酶的活性增加，或者乌头酸酶和乙醛脱氢酶的活性 在培养基中增加，并从细菌的培养基或细胞中收集L-氨基酸。
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