公开(公告)号：US09822385B2

公开(公告)日：2017-11-21

申请号：US14723776

申请日：2015-05-28

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

CPC classification number: C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

公开(公告)号：US20150203881A1

公开(公告)日：2015-07-23

申请号：US14668080

申请日：2015-03-25

Applicant: AJINOMOTO CO., INC.

Inventor： Leonid Romanovich Ptitsyn ,  Irina Borisovna Altman ,  Veronika Aleksandrovna Kotliarova ,  Olga Nikolaevna Mokhova ,  Tatyana Abramovna Yampolskaya ,  Yury Ivanovich Kozlov ,  Masaru Terashita ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/08 ,  C12P13/06

CPC classification number: C12P13/08 ,  C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24 ,  C12Y101/01002

Abstract: A method for producing an L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of a wild-type alcohol dehydrogenase encoded by the adhE gene or a mutant alcohol dehydrogenase which is resistant to aerobic inactivation.

Abstract translation: 例如使用肠杆菌科的细菌来描述L-氨基酸的制造方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸，L-色氨酸或L-谷氨酸 家族，其中细菌已经被修饰以增强由adhE基因编码的野生型醇脱氢酶的活性或对有氧失活有抗性的突变型醇脱氢酶。

公开(公告)号：US20150175954A1

公开(公告)日：2015-06-25

申请号：US14551952

申请日：2014-11-24

Applicant: AJINOMOTO CO., INC.

Inventor： Kazuhiko Kunita ,  Tomoaki Tanaka ,  Yoko Kuwabara ,  Yoshihiro Usuda

IPC: C12N1/12 ,  C12P7/64 ,  C12P1/00

CPC classification number: C12N1/12 ,  A23K10/12 ,  C12N1/005 ,  C12P1/00 ,  C12P7/6409

Abstract: A medium component for more efficiently culturing a microorganism or microalga is provided. A nutrient additive useful in culturing a microorganism or microalga is produced by culturing a microalga in a medium to produce biomass derived from the microalga in the medium, adding an acid to the biomass to hydrolyze the biomass, and preparing the hydrolysate of the biomass as a nutrient additive.

Abstract translation: 提供了用于更有效地培养微生物或微藻的培养基成分。 用于培养微生物或微藻的营养添加剂通过在培养基中培养微藻来生产源自培养基中的微藻的生物质，向生物质中加入酸以水解生物质，并将生物质的水解产物作为 营养添加剂。

公开(公告)号：US20230212503A1

公开(公告)日：2023-07-06

申请号：US18063181

申请日：2022-12-08

Applicant: AJINOMOTO CO., INC.

Inventor： Nobuhisa Nitta ,  Hiroaki Fukada ,  Kazutaka Shimbo ,  Akiko Matsudaira ,  Toshiki Watanabe ,  Yoshihiro Usuda

IPC: C12N1/14

CPC classification number: C12N1/145 ,  C12N2501/734

Abstract: A method for producing a protein is provided. An objective protein is produced by culturing in a culture medium Talaromyces cellulolyticus having an objective protein-producing ability, which has been modified so that the activity of a Pep4 protein is reduced.

公开(公告)号：US10563234B2

公开(公告)日：2020-02-18

申请号：US15361645

申请日：2016-11-28

Applicant: AJINOMOTO CO., INC.

Inventor： Hidetaka Doi ,  Akiko Matsudaira ,  Yoshihiro Usuda

IPC: C12P13/04 ,  C12P13/08

Abstract: A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, wherein the bacterium has been modified so that the activity of aconitase is increased, or the activities of aconitase and acetaldehyde dehydrogenase are increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.

公开(公告)号：US20170073714A1

公开(公告)日：2017-03-16

申请号：US15361645

申请日：2016-11-28

Applicant: AJINOMOTO CO., INC.

Inventor： Hidetaka Doi ,  Akiko Matsudaira ,  Yoshihiro Usuda

IPC: C12P13/08

CPC classification number: C12P13/08 ,  C12N15/00 ,  C12N15/09

Abstract: A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, wherein the bacterium has been modified so that the activity of aconitase is increased, or the activities of aconitase and acetaldehyde dehydrogenase are increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.

Abstract translation: 提供了L-氨基酸的制造方法。 通过培养属于肠杆菌科的细菌并具有L-氨基酸生产能力来生产L-氨基酸，其中细菌已被修饰，使得乌头酸酶的活性增加，或者乌头酸酶和乙醛脱氢酶的活性 在培养基中增加，并从细菌的培养基或细胞中收集L-氨基酸。

公开(公告)号：US20150259717A1

公开(公告)日：2015-09-17

申请号：US14723776

申请日：2015-05-28

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/14

CPC classification number: C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

Abstract translation: 具有羧基的酸性物质是通过在培养基中培养已被改良以增强ybjL基因表达的微生物，并从培养基中收集具有羧基的酸性物质产生的。

公开(公告)号：US20130316401A1

公开(公告)日：2013-11-28

申请号：US13939291

申请日：2013-07-11

Applicant: AJINOMOTO CO., INC.

Inventor： Yosuke Nishio ,  Eiichiro Kimura ,  Yoshihiro Usuda ,  Kazuho Ikeo ,  Yoji Nakamura ,  Takashi Gojobori ,  Yutaka Kawarabayashi ,  Yumi Hino ,  Eiichi Hori ,  Jun Yamazaki

IPC: C12N9/88 ,  C12N9/12 ,  C12N9/06

CPC classification number: C12N9/88 ,  C12N9/00 ,  C12N9/0016 ,  C12N9/1205 ,  C12N15/1034 ,  C12N15/1089

Abstract: A method for improving the thermostability of a protein includes introducing, into the protein, two or more amino acid substitutions selected from the group consisting of: (i) substitution of an arginine residue for a lysine residue, (ii) substitution of a threonine residue for a serine residue, and (iii) substitution of an alanine residue for a serine residue.

Abstract translation: 提高蛋白质热稳定性的方法包括向蛋白质中引入选自以下的两个或多个氨基酸取代：（i）精氨酸残基取代赖氨酸残基，（ii）苏氨酸残基的取代 用于丝氨酸残基，和（iii）用丙氨酸残基取代丝氨酸残基。

公开(公告)号：US20140045228A1

公开(公告)日：2014-02-13

申请号：US14027365

申请日：2013-09-16

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/08

CPC classification number: C12P13/08 ,  C12P13/04 ,  C12P13/14 ,  C12P13/227

Abstract: An L-amino acid is produced by culturing an Enterobacteriaceae which is able to produce an L-amino acid in a medium containing glycerol, especially crude glycerol, as the carbon source to produce and accumulate the L-amino acid in the culture, and collecting the L-amino acid from the culture.

Abstract translation: 通过培养能够在含有甘油，特别是粗甘油的培养基中产生L-氨基酸的肠杆菌科生产L-氨基酸作为碳源，以在培养物中产生和积累L-氨基酸，并收集 来自培养物的L-氨基酸。

公开(公告)号：US20130260425A1

公开(公告)日：2013-10-03

申请号：US13912313

申请日：2013-06-07

Applicant: Ajinomoto Co., Inc.

Inventor： Hidetaka Doi ,  Yasushi Hoshino ,  Yuri Masumitsu ,  Yoshihiro Usuda

IPC: C12P13/08 ,  C12P13/04

CPC classification number: C12P13/08 ,  C07K14/245 ,  C12N1/20 ,  C12N1/38 ,  C12N15/746 ,  C12P13/04

Abstract: A method for producing an L-amino acid includes culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium. A bacterium which has been subjected to a modification including at least one of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof, is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.

Abstract translation: L-氨基酸的制造方法包括在含有选自脂肪酸和醇的碳源的培养基中培养属于肠杆菌科的细菌并具有L-氨基酸生产能力的细菌， 来自培养基的氨基酸。 使用已经进行了包括oxyS基因表达增强，固定化ABC基因表达的增强及其组合中的至少一种的修饰的细菌作为细菌，或者添加减少细菌的细胞内过氧化氢浓度的物质 到中等