公开(公告)号：US10941390B2

公开(公告)日：2021-03-09

申请号：US16897482

申请日：2020-06-10

Applicant: AJINOMOTO CO., INC.

Inventor： Noriko Yokoyama ,  Mayu Nakano ,  Shuhei Hashiro ,  Masako Hiraga ,  Kazutaka Shimbo ,  Ayaka Tokunaga

IPC: C12N9/82 ,  A23L27/60 ,  A23L13/00 ,  A23C13/16 ,  C12P21/00 ,  A21D2/26 ,  A23C9/12 ,  A23L13/40 ,  A23L11/00

Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.

公开(公告)号：US20210024931A1

公开(公告)日：2021-01-28

申请号：US16993692

申请日：2020-08-14

Applicant: AJINOMOTO CO., INC

Inventor： Shuhei Hashiro ,  Hisashi Yasueda ,  Mayu Nakano ,  Teruyuki Niimi ,  Haruka Kawaguchi

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/70 ,  C12N15/77 ,  A01N63/10

Abstract: A method for efficiently inducing RNA silencing in a target organism is provided. RNA silencing is induced in a target organism by allowing the target organism to ingest cells of a microorganism, wherein the microorganism is able to produce RNA that induces RNA silencing after treating the cells with an organic solvent.

公开(公告)号：US20200318093A1

公开(公告)日：2020-10-08

申请号：US16897482

申请日：2020-06-10

Applicant: AJINOMOTO CO., INC.

Inventor： Noriko YOKOYAMA ,  Mayu Nakano ,  Shuhei Hashiro ,  Masako Hiraga ,  Kazutaka Shimbo ,  Ayaka Tokunaga

IPC: C12N9/82 ,  A23C13/16 ,  C12P21/00 ,  A21D2/26 ,  A23C9/12 ,  A23L27/60 ,  A23L13/40 ,  A23L11/00

Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.

公开(公告)号：US10717971B2

公开(公告)日：2020-07-21

申请号：US15252664

申请日：2016-08-31

Applicant: AJINOMOTO CO., INC.

Inventor： Noriko Yokoyama ,  Mayu Nakano ,  Shuhei Hashiro ,  Masako Hiraga ,  Kazutaka Shimbo ,  Ayaka Tokunaga

IPC: C12N9/82 ,  C12N15/52 ,  C12N9/78 ,  A23C13/16 ,  C12P21/00 ,  A21D2/26 ,  A23C9/12 ,  A23L27/60 ,  A23L13/40 ,  A23L11/00

Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.