公开(公告)号：US20240165260A1

公开(公告)日：2024-05-23

申请号：US18523499

申请日：2023-11-29

Applicant: Ajinomoto Co., Inc.

Inventor： Yutaka MATSUDA ,  Tomohiro FUJII ,  Ryusuke HIRAMA ,  Thomas MARKOTAN ,  Allan PRIOR ,  Christopher WONG ,  Konstantinos PLAKAS ,  Tomohiro WATANABE

IPC: A61K47/68 ,  C07K5/062

CPC classification number: A61K47/6889 ,  A61K47/68031 ,  A61K47/6855 ,  C07K5/06052

Abstract: A conjugate of an antibody and a functional substance or a salt thereof, which comprises a structural unit represented by the following formula (I):






wherein Ig represents an immunoglobulin unit comprising two heavy chains and two light chains, and regioselectively forms an amide bond with a carbonyl group adjacent to Ig via an amino group in side chains of lysine residues in the two heavy chains,
L1 and L2 each represent a divalent group,
R1 represents a monovalent group optionally comprising a hydrophilic group,
X represents a predetermined divalent group,
D represents a functional substance,
RA represents a side chain of a valine residue,
RB represents a side chain of a citrulline residue or an alanine residue,
n is 0 or 1, and
an average ratio r of the amide bonds per two heavy chains is 1.5 to 2.5, and
wherein at least one hydrophilic group is present in the structural unit are excellent in desired properties while controlling a binding ratio between the antibody and the functional substance within a desired range.

公开(公告)号：US20240115719A1

公开(公告)日：2024-04-11

申请号：US18463903

申请日：2023-09-08

Applicant: Ajinomoto Co., Inc.

Inventor： Tomohiro FUJII ,  Kei YAMADA ,  Yutaka MATSUDA ,  Ryusuke HIRAMA ,  Noriko HATADA ,  Naoko ARASHIDA

IPC: A61K47/68 ,  C07K16/28

CPC classification number: A61K47/68031 ,  C07K16/2803

Abstract: Compounds or salts thereof represented by formula (I):






wherein
X indicates a leaving group,
Y indicates an affinity peptide having a binding region in a CH2 domain in an immunoglobulin unit comprising two heavy chains and two light chains,
M indicates a trivalent group linking the carbon atom in C═O adjacent to M and the carbon atom in C═W via a main chain portion consisting of 3 to 5 carbon atoms,
O indicates an oxygen atom,
S indicates a sulfur atom,
W indicates an oxygen atom or a sulfur atom,
N3 indicates an azide group,
La indicates a bond or a divalent group, and
Lb indicates a bond or a divalent group; and antibodies or salts thereof which can be prepared using such a compound or salt thereof, are useful for controlling the bonding ratio of an antibody and a modifying group within a desired range.

公开(公告)号：US20240000964A1

公开(公告)日：2024-01-04

申请号：US18354256

申请日：2023-07-18

Applicant: Ajinomoto Co., Inc.

Inventor： Tomohiro FUJII ,  Kei YAMADA ,  Yutaka MATSUDA ,  Ryusuke HIRAMA ,  Noriko HATADA

IPC: A61K47/68 ,  C07K16/32 ,  C07C247/16 ,  C07D271/113 ,  C07D213/71 ,  C07D225/08 ,  C07D403/12 ,  C07D401/12

CPC classification number: A61K47/6889 ,  C07K16/32 ,  A61K47/6855 ,  C07C247/16 ,  C07D271/113 ,  C07D213/71 ,  C07D225/08 ,  C07D403/12 ,  C07D401/12 ,  C07K2317/51 ,  C07K2317/52

Abstract: A desired antibody or antibody composition may be obtained by modifying a thiol group chemically introduced to an antibody to obtain an antibody composition comprising



(A) an antibody intermediate or a salt thereof having a bioorthogonal functional group which may be protected, which is represented by the following formula (A):






AbS-L-R]n  (A)





wherein
Ab is a certain antibody,
S is a sulfur atom,
L is a divalent group,
R is a bioorthogonal functional group which may be protected, and
n is an integer from 1 to 8, and


(B) a thiol group-introduced antibody or a salt thereof, which is represented by the following formula (B):






AbSH]n  (B)





wherein
Ab and n are the same as formulae (A), and
SH is a thiol group; and the like.

公开(公告)号：US20210009941A1

公开(公告)日：2021-01-14

申请号：US17036476

申请日：2020-09-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ryusuke HIRAMA ,  Riho KODAMA ,  Manabu KITAZAWA ,  Nao SUGIMOTO ,  Hiroki OZAWA

IPC: C12N5/00 ,  C08G63/91 ,  C12N5/074

Abstract: The present invention provides a composition for promoting cell proliferation containing polylysine in which at least one amino group is mono-substituted by dicarboxylic acid.