公开(公告)号：US20180195103A1

公开(公告)日：2018-07-12

申请号：US15911731

申请日：2018-03-05

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Takayuki ITO ,  Hiroyuki NOZAKI

IPC: C12P21/02 ,  C12R1/19 ,  C12N9/00 ,  C07K5/083 ,  C07K5/093

CPC classification number: C12P21/02 ,  C07K5/0806 ,  C07K5/0819 ,  C12N9/93 ,  C12N15/09 ,  C12R1/19 ,  C12Y603/02002 ,  C12Y603/02003

Abstract: A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDK) is reduced as an expression host, γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.

公开(公告)号：US20160326510A1

公开(公告)日：2016-11-10

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12N9/00 ,  C07K5/02 ,  C12P21/02

CPC classification number: C12N9/93 ,  C07K5/0215 ,  C12P21/02 ,  C12Y603/02002

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

Abstract translation: 提供适用于产生γ-Glu-Val的突变型谷氨酸 - 半胱氨酸连接酶（GSHA），以及使用其制备γ-Glu-Val-Gly的方法。 通过使用与Glu和Val作为原料具有特异性突变的突变体GSHA，通过使用γ-Glu-Val和Gly作为原料进一步制备γ-Glu-Val-Gly来产生γ-Glu-Val。 通过使用与Glu，Val和Gly作为原料具有特异性突变的突变体GSHA来产生γ-Glu-Val-Gly。

公开(公告)号：US20230399673A1

公开(公告)日：2023-12-14

申请号：US18454052

申请日：2023-08-22

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Takayuki ITO ,  Hiroyuki NOZAKI

IPC: C12P21/02 ,  C12N1/20 ,  C07K5/083 ,  C07K5/093 ,  C12N9/00

CPC classification number: C12P21/02 ,  C12N1/205 ,  C07K5/0806 ,  C07K5/0819 ,  C12N9/93 ,  C12N15/09

Abstract: A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDK) is reduced as an expression host, γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.