公开(公告)号：US09587259B2

公开(公告)日：2017-03-07

申请号：US14467459

申请日：2014-08-25

Applicant: AJINOMOTO CO., INC.

Inventor： Fumi Shozui ,  Yoshinori Tajima

IPC: C12P13/00 ,  C08G73/22 ,  C12N15/70 ,  C12N9/88 ,  C12N9/10

CPC classification number: C12P13/005 ,  C08G73/22 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/70 ,  C12Y203/01118 ,  C12Y401/99

Abstract: The present invention provides a method for conveniently and inexpensively producing an amino-hydroxybenzoic acid-type compound such as 3-amino-4-hydroxybenzoic acid by utilizing Escherichia coli that is a bacterium commonly used for a production process by biosynthesis. Specifically, the present invention provides an Escherichia coli having an ability to produce 3-amino-4-hydroxybenzoic acid, which is modified to reduce an activity of N-hydroxyarylamine O-acetyltransferase (NhoA); a method for producing a 3-amino-4-hydroxybenzoic acid-type compound using such Escherichia coli; and the like.

Abstract translation: 本发明提供了通过利用作为通常用于通过生物合成的生产方法的细菌的大肠杆菌来方便和廉价地制备氨基 - 羟基苯甲酸型化合物如3-氨基-4-羟基苯甲酸的方法。 具体地说，本发明提供具有生产3-氨基-4-羟基苯甲酸的能力的大肠杆菌，其被修饰以降低N-羟基芳基胺O-乙酰基转移酶（NhoA）的活性; 使用这样的大肠杆菌生产3-氨基-4-羟基苯甲酸型化合物的方法; 等等。

公开(公告)号：US08962296B2

公开(公告)日：2015-02-24

申请号：US13832260

申请日：2013-03-15

Applicant: Bridgestone Corporation ,  Ajinomoto Co., Inc.

Inventor： Yasuyuki Hayashi ,  Minako Harada ,  Saaya Takaoka ,  Yasuo Fukushima ,  Keiichi Yokoyama ,  Yosuke Nishio ,  Yoshinori Tajima ,  Yoko Mihara ,  Kunio Nakata

IPC: C12N9/88 ,  C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C12N1/20 ,  C12N1/00 ,  C12P5/02 ,  C07H21/04 ,  C07K1/00 ,  C12P5/00

CPC classification number: C12P5/007 ,  C12N9/88 ,  C12P5/00 ,  C12Y402/03027

Abstract: The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c): (a) a polynucleotide comprising (i) the nucleotide sequence represented by SEQ ID NO:1, or (ii) the nucleotide sequence consisting of the nucleotide residues at positions 133 to 1785 in the nucleotide sequence represented by SEQ ID NO:1; (b) a polynucleotide that comprises a nucleotide sequence having 90% or more identity to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; or (c) a polynucleotide that hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; and the like.

Abstract translation: 本发明提供了用于建立优异的异戊二烯单体生产系统的方法。 具体而言，本发明提供下述（a），（b）或（c）所示的多核苷酸：（a）包含（i）由SEQ ID NO：1表示的核苷酸序列的多核苷酸，或（ii） 由SEQ ID NO：1所示的核苷酸序列的133〜1785位核苷酸残基组成的序列; （b）包含与上述（i）或（ii）的核苷酸序列具有90％以上同一性的核苷酸序列的多核苷酸，并编码具有异戊二烯合酶活性的蛋白质; 或（c）在严格条件下与由上述（i）或（ii）的核苷酸序列互补的核苷酸序列组成的多核苷酸杂交的多核苷酸，并编码具有异戊二烯合酶活性的蛋白质; 等等。

公开(公告)号：US20210071210A1

公开(公告)日：2021-03-11

申请号：US17036054

申请日：2020-09-29

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Mihara ,  Yohei Tatsukami ,  Yoshinori Tajima

IPC: C12P7/42 ,  C12P17/06

Abstract: The present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, productivity of which has been enhanced. Specifically, the present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, by producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein such as the following: (A) a protein having an amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14; (B) a protein having an amino acid sequence containing one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14, and having a linoleate 13-hydratase activity; and (C) a protein having an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13 or 14, and having a linoleate 13-hydratase activity.

公开(公告)号：US20180044703A1

公开(公告)日：2018-02-15

申请号：US15787861

申请日：2017-10-19

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/14 ,  C12P7/50 ,  C12P7/48 ,  C12P7/46 ,  C07K14/265 ,  C07K14/245 ,  C12P13/20 ,  C07K14/195

CPC classification number: C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

公开(公告)号：US20150079644A1

公开(公告)日：2015-03-19

申请号：US14547481

申请日：2014-11-19

Applicant: AJINOMOTO CO., INC.

Inventor： Fumi Shozui ,  Yoshinori Tajima ,  Keiichi Yokoyama

IPC: C12P13/02 ,  C08G73/22

CPC classification number: C12P13/02 ,  C08G73/22 ,  C12N9/104 ,  C12P13/005 ,  Y02P20/52

Abstract: The present invention provides a method for conveniently and efficiently producing a 3-acetylamino-4-hydroxybenzoic acid-type compound that is a stable compound by a process using a microorganism. Specifically the present invention provides a microorganism having an ability to produce 3-amino-4-hydroxybenzoic acid, that is modified so as to increase an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde, wherein the microorganism is modified so as to increase an N-hydroxyarylamine O-acetyltransferase (NhoA) activity, as well as a method for producing the 3-acetylamino-4-hydroxybenzoic acid-type compound using such a microorganism.

Abstract translation: 本发明提供了通过使用微生物的方法方便有效地制备作为稳定化合物的3-乙酰氨基-4-羟基苯甲酸型化合物的方法。 具体地说，本发明提供具有生产3-氨基-4-羟基苯甲酸的能力的微生物，其被修饰以增加从磷酸二羟丙酮和天冬氨酸半醛形成3-氨基-4-羟基苯甲酸的活性，其中微生物 被修饰以增加N-羟基芳基胺O-乙酰基转移酶（NhoA）活性，以及​​使用这种微生物生产3-乙酰氨基-4-羟基苯甲酸型化合物的方法。

公开(公告)号：US20140364579A1

公开(公告)日：2014-12-11

申请号：US14467459

申请日：2014-08-25

Applicant: AJINOMOTO CO., INC.

Inventor： Fumi Shozui ,  Yoshinori Tajima

IPC: C12P13/00 ,  C08G73/22

CPC classification number: C12P13/005 ,  C08G73/22 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/70 ,  C12Y203/01118 ,  C12Y401/99

Abstract: The present invention provides a method for conveniently and inexpensively producing an amino-hydroxybenzoic acid-type compound such as 3-amino-4-hydroxybenzoic acid by utilizing Escherichia coli that is a bacterium commonly used for a production process by biosynthesis. Specifically, the present invention provides an Escherichia coli having an ability to produce 3-amino-4-hydroxybenzoic acid, which is modified to reduce an activity of N-hydroxyarylamine O-acetyltransferase (NhoA); a method for producing a 3-amino-4-hydroxybenzoic acid-type compound using such Escherichia coli; and the like.

Abstract translation: 本发明提供了通过利用作为通常用于通过生物合成的生产方法的细菌的大肠杆菌来方便和廉价地制备氨基 - 羟基苯甲酸型化合物如3-氨基-4-羟基苯甲酸的方法。 具体地说，本发明提供具有生产3-氨基-4-羟基苯甲酸的能力的大肠杆菌，其被修饰以降低N-羟基芳基胺O-乙酰基转移酶（NhoA）的活性; 使用这样的大肠杆菌生产3-氨基-4-羟基苯甲酸型化合物的方法; 等等。

公开(公告)号：US09890373B2

公开(公告)日：2018-02-13

申请号：US15159140

申请日：2016-05-19

Applicant: AJINOMOTO CO., INC.

Inventor： Kunio Nakata ,  Yoshinori Tajima ,  Uno Tagami ,  Takashi Oku ,  Yasuhiro Kashima

IPC: C12N9/88 ,  C08F36/08 ,  C12N15/09 ,  C12P5/02 ,  C08K3/04 ,  C08K3/22 ,  C12P5/00

CPC classification number: C12N9/88 ,  C08F36/08 ,  C08K3/04 ,  C08K3/22 ,  C08K2003/2296 ,  C12N15/09 ,  C12P5/007 ,  C12P5/026 ,  C12Y402/03007 ,  C12Y402/03027 ,  C08L9/00

Abstract: The present application relates to a modified isoprene synthase that has an isoprene synthetic activity and has at least one mutation of an amino acid residue in the amino acid sequence of SEQ ID NO: 4, an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO: 4, or an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO: 4. The modified isoprene synthase is useful for preparing isoprene monomers in improved yields.

公开(公告)号：US09822385B2

公开(公告)日：2017-11-21

申请号：US14723776

申请日：2015-05-28

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

CPC classification number: C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

公开(公告)号：US09447443B2

公开(公告)日：2016-09-20

申请号：US14547481

申请日：2014-11-19

Applicant: AJINOMOTO CO., INC.

Inventor： Fumi Shozui ,  Yoshinori Tajima ,  Keiichi Yokoyama

IPC: C12P13/02 ,  C12P7/22 ,  C12P7/42 ,  C12N9/10 ,  C12P13/00 ,  C08G73/22

CPC classification number: C12P13/02 ,  C08G73/22 ,  C12N9/104 ,  C12P13/005 ,  Y02P20/52

Abstract: The present invention provides a method for conveniently and efficiently producing a 3-acetylamino-4-hydroxybenzoic acid-type compound that is a stable compound by a process using a microorganism. Specifically the present invention provides a microorganism having an ability to produce 3-amino-4-hydroxybenzoic acid, that is modified so as to increase an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde, wherein the microorganism is modified so as to increase an N-hydroxyarylamine O-acetyltransferase (NhoA) activity, as well as a method for producing the 3-acetylamino-4-hydroxybenzoic acid-type compound using such a microorganism.

Abstract translation: 本发明提供了通过使用微生物的方法方便有效地制备作为稳定化合物的3-乙酰氨基-4-羟基苯甲酸型化合物的方法。 具体地说，本发明提供具有生产3-氨基-4-羟基苯甲酸的能力的微生物，其被修饰以增加从磷酸二羟丙酮和天冬氨酸半醛形成3-氨基-4-羟基苯甲酸的活性，其中微生物 被修饰以增加N-羟基芳基胺O-乙酰基转移酶（NhoA）活性，以及​​使用这种微生物生产3-乙酰氨基-4-羟基苯甲酸型化合物的方法。

公开(公告)号：US11499171B2

公开(公告)日：2022-11-15

申请号：US17036054

申请日：2020-09-29

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Mihara ,  Yohei Tatsukami ,  Yoshinori Tajima

IPC: C12P7/42 ,  C12N9/88 ,  C12P17/06

Abstract: The present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, productivity of which has been enhanced. Specifically, the present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, by producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein such as the following: (A) a protein having an amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14; (B) a protein having an amino acid sequence containing one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14, and having a linoleate 13-hydratase activity; and (C) a protein having an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13 or 14, and having a linoleate 13-hydratase activity.