公开(公告)号：US20190382773A1

公开(公告)日：2019-12-19

申请号：US15771888

申请日：2016-10-28

申请人： AMYRIS, INC.

发明人： Andrew MAIN ,  Grzegorz WOJCIECHOWSKI ,  Yue YANG ,  Lishan ZHAO

IPC分类号： C12N15/52 ,  C12P5/00

摘要： Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase. Also provided herein are isolated nude is acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US20220002735A1

公开(公告)日：2022-01-06

申请号：US17371678

申请日：2021-07-09

申请人： AMYRIS, INC.

发明人： Andrew MAIN ,  Grzegorz WOJCIECHOWSKI ,  Yue YANG ,  Lishan ZHAO

IPC分类号： C12N15/52 ,  C12P5/00 ,  C12N1/20 ,  C12N1/14 ,  C12N1/16

摘要： Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US20210355458A1

公开(公告)日：2021-11-18

申请号：US17382963

申请日：2021-07-22

申请人： AMYRIS, INC.

发明人： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC分类号： C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

摘要： Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US20190185826A1

公开(公告)日：2019-06-20

申请号：US16323756

申请日：2017-08-11

申请人： AMYRIS, INC.

发明人： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  GARCIA DE GONZALO, Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC分类号： C12N9/10 ,  C12N15/52 ,  C12P19/56 ,  C12P19/18 ,  A23L2/60 ,  A23L27/30

CPC分类号： C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017

摘要： Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.